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公开(公告)号：KR100958545B1
公开(公告)日：2010-05-18
申请号：KR1020070104948
申请日：2007-10-18
Applicant: 한국과학기술연구원
IPC: C12Q1/68
Abstract: 본 발명은 다환 방향족 탄화수소류 중의 하나인 벤조케이플루오란텐(benzo[k]fluoranthene)에 대한 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 구체적으로 벤조케이플루오란텐에 의해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 벤조케이플루오란텐에 대한 노출 여부를 확인하는 방법에 관한 것이다. 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 벤조케이플루오란텐의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 벤조케이플루오란텐에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
벤조케이플루오란텐, 다환 방향족 탄화수소, 바이오마커, 마이크로 어레이-
公开(公告)号：KR100915898B1
公开(公告)日：2009-09-07
申请号：KR1020070017534
申请日：2007-02-21
Applicant: 한국과학기술연구원
IPC: C12Q1/68
Abstract: 본 발명은 다환 방향족 탄화수소류 중의 하나인 3-메틸콜란트렌(3-methylcholanthrene)에 대한 노출 여부 진단용 바이오마커 및 이를 이용한 진단 방법에 관한 것으로, 구체적으로 3-메틸콜란트렌에 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 3-메틸콜란트렌에 대한 노출 여부를 진단하는 방법에 관한 것이며, 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 3-메틸콜란트렌의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 3-메틸콜란트렌에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
3-메틸콜란트렌, 다환 방향족 탄화수소, 마이크로어레이 칩-
公开(公告)号：KR1020090039354A
公开(公告)日：2009-04-22
申请号：KR1020070104948
申请日：2007-10-18
Applicant: 한국과학기술연구원
IPC: C12Q1/68
CPC classification number: C12Q1/6883 , C12Q1/6827 , C12Q1/6886 , C40B40/08
Abstract: A biomarker for detecting exposure to benzo[k]fluoranthene is provided to monitor and detect benzo[k]fluoranthene contamination in environmental sample and examine toxicity mechanism caused by benzo[k]fluoranthene. A biomarker for detecting exposure to benzo[k]fluoranthene comprises a gene whose expression is increased or decreased by the exposure to the benzo[k]fluoranthene. A DNA microarray chip is integrated with oligo-nucleotide comprising entire or a part of biomarker gene sequence or its complementary sequence. A method for detecting the exposure to the benzo[k]fluoranthene comprises: a step of collecting cell from a target; a step of RNA isolation from the collected cell as a experimental group and human cell untreated as a control group; a step of synthesizing the RNA of experimental group and control group with cDNA and probing with different fluorescence; a step of hybridizing the fluorescence attached cDNA with DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing expression level of biomarker with the control group and judging exposure to benzo[k]fluoranthene.
Abstract translation: 提供了一种用于检测苯并[k]荧蒽暴露的生物标志物，用于监测和检测环境样品中的苯并[k]荧蒽污染物，并检测苯并[k]荧蒽产生的毒性机制。 用于检测暴露于苯并[k]荧蒽的生物标志物包括通过暴露于苯并[k]荧蒽而表达增加或降低的基因。 将DNA微阵列芯片与包含全部或部分生物标记基因序列或其互补序列的寡核苷酸整合。 检测对苯并[k]荧蒽的暴露的方法包括：从目标物收集细胞的步骤; 作为实验组从收集的细胞中分离RNA的步骤和未处理作为对照组的人细胞; 用cDNA合成实验组和对照组的RNA并用不同荧光探测的步骤; 将荧光连接的cDNA与DNA微阵列芯片杂交的步骤; 分析DNA微阵列芯片的步骤; 比较生物标志物与对照组的表达水平并判断苯并[k]荧蒽的暴露的步骤。
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公开(公告)号：KR1020080077834A
公开(公告)日：2008-08-26
申请号：KR1020070017534
申请日：2007-02-21
Applicant: 한국과학기술연구원
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q2563/107 , C12Q2600/158
Abstract: A biomarker selected through a DNA microarray chip is provided to be usefully used to monitor and determine 3-methylcholanthrene contamination and be used as a tool for identifying a toxicity mechanism induced by the 3-methylcholanthrene. A biomarker for diagnosing exposure to 3-methylcholanthrene includes a gene selected from the group consisting of Genebank NOs. NM_000499, NM_203347, NM_006227, NM_018837, NM_001769, NM_001402, NM_170693, NM_021599, NM_001017973, NM_032324, NM_198194, NM_016368, NM_030569, NM_001748, NM_012467, NM_001917, NM_015920, NM_001009820, NM_014010, NM_152640, NM_001019, NM_002842, NM_001004, NM_006929, NM_012190, NM_032872, NM_000480, NM_000476, NM_018161, NM_021063, NM_001005749, NM_005319, NM_001015053, NM_003528, NM_002778, NM_021058, NM_003524, NM_003519, NM_033445, NM_005572, NM_003520, NM_003527, NM_175055, NM_000596, NM_004864, NM_201525, NM_014624, NM_175744, NM_005620, NM_006018, NM_005310, NM_002926, NM_021913, NM_002194, BC064982, NM_001005339, NM_021077, NM_004881, NM_018494, NM_033285, NM_001197, NM_001540, NM_003820, NM_147780, NM_001003940, NM_000389, NM_016639, NM_000043, NM_002307, NM_021603, NM_004925, NM_000014, NM_022449, NM_005855, NM_006868, NM_032493, NM_007097, NM_005697, NM_004073, NM_182795, NM_005072, NM_144606, NM_014059, NM_002754, NM_003254, NM_001553, NM_003255, NM_013376, NM_000107, NM_004433, NM_021969, NM_023039, NM_080875, NM_002166, NM_001421, NM_153813, U68019, NM_015655, NM_031918, NM_001554, NM_003960, NM_032965, NM_201397, NM_005064, NM_005345, NM_001531, NM_006404, NM_002119, NM_005101, NM_006426, NM_020728, NM_016441, NM_203391, NM_006775, NM_052937, NM_003932, NM_030979, NM_032549, NM_007195, NM_002139, C033021, NM_016093, CR611166, NM_017437, NM_033114, NM_000236, NM_174936, NM_004375, NM_004685, NM_052965, NM_201278, NM_003093, AV757313, NM_014791, BX537987, BC041925, BC032643, NM_016058, NM_181886, NM_016271, AK021676, NM_015352, NM_016304, NM_020995, NM_003017, W04231, NM_006546, NM_212554, BC049823, NM_006937, NM_012331, NM_005875, NM_032906, NM_002847, NM_000947, NM_181716, NM_004681, NM_000982, T07777, NM_016652, NM_000986, NM_139207, AK001406, NM_145649, NM_020236, NM_001017430, NM_031157, NM_018981, NM_018291, NM_018048, NM_012207, NM_000028, NM_014321, NM_015423, NM_001034, AB032990, NM_000791, NM_199040, NM_006886, NM_004457, NM_007295, NM_006111, NM_001443, NM_000016, NM_001809, AL833119, NM_006493, NM_138271, NM_006136, CR615278, NM_024704, NM_003011, NM_203401, CR749233, AF277624, NM_013282, NM_198893, NM_013361, NM_002129, NM_002128, NM_003429, NM_003423, NM_016220, NM_022103, NM_021269, NM_016649, NM_199132, AK098175, NM_198381, NM_138330, NM_030824, NM_003441, NM_001001415, AK128731, NM_203282, CR627133, NM_007139, NM_178558, NM_024498, NM_003430, NM_003410, NM_178549, NM_152601, NM_024629, BC015987, NM_145233, NM_031942, NM_133473, NM_005655, NM_021994, NM_025189, NM_001008390, NM_024087, NM_032828, NM_006980, NM_024561, NM_173531, NM_003864, NM_012345, NM_003079, NM_006193, NM_003599, NM_006311, NM_020675, NM_018492, NM_145697, NM_006101, NM_002358, NM_006716, NM_003318, NM_018131, NM_012177, NM_013277, NM_018136, NM_014750, NM_016343, NM_017489, NM_006461, NM_001790, NM_004064, NM_080668, NM_021211, NM_001274, , NM_001005414, NM_144710, NM_004336, NM_005496, NM_181803, NM_018101, AF147440, NM_005030, NM_022346, NM_019084, NM_005504, NM_004523, NM_004354, NM_001237, NM_032626, NM_021930, NM_018685, NM_016195, NM_001827, NM_138555, NM_031966, NM_005915, NM_014751, NM_003981, NM_001826, NM_198219, NM_014881, NM_001255, NM_016359, AF085846, NM_001656, NM_145307, NM_080651, NM_003714, NM_018098, NM_004586, NM_002317, NM_031296, NM_014736, XM_292197, NM_016513, NM_001331, AB032991, NM_004232, NM_003472, NM_012334, NM_012425, NM_002140, NM_005271, NM_145203, BU679059, NM_006305, NM_020824, NM_012120, NM_012089, NM_021977, NM_015171, NM_016467, AA484677, BC035622, NM_002520, NM_014043, NM_003133, NM_013322, NM_005733, NM_014322, NM_020401, NM_138285, NM_001786, NM_001067, NM_001012271, NM_024854, NM_018204, NM_021999, NM_005400, NM_148957, NM_001006, NM_006437, NM_024055, NM_000582, NM_016951, NM_002737, NM_004836, NM_020686, NM_004134, NM_053039, NM_007195, NM_005256, NM_003368, NM_001018115, AI281523, NM_004075, NM_002389, NM_000584, NM_001801, NM_000715, NM_144503, NM_145697, and NM_001010914.
Abstract translation: 提供通过DNA微阵列芯片选择的生物标志物，以有效地用于监测和测定3-甲基胆蒽污染物，并用作识别3-甲基胆蒽诱导的毒性机制的工具。 用于诊断3-甲基胆蒽暴露的生物标志物包括选自Genebank NOs的基因。 NM_000499，NM_203347，NM_006227，NM_018837，NM_001769，NM_001402，NM_170693，NM_021599，NM_001017973，NM_032324，NM_198194，NM_016368，NM_030569，NM_001748，NM_012467，NM_001917，NM_015920，NM_001009820，NM_014010，NM_152640，NM_001019，NM_002842，NM_001004，NM_006929，NM_012190， NM_032872，NM_000480，NM_000476，NM_018161，NM_021063，NM_001005749，NM_005319，NM_001015053，NM_003528，NM_002778，NM_021058，NM_003524，NM_003519，NM_033445，NM_005572，NM_003520，NM_003527，NM_175055，NM_000596，NM_004864，NM_201525，NM_014624，NM_175744，NM_005620，NM_006018， NM_005310，NM_002926，NM_021913，NM_002194，BC064982，NM_001005339，NM_021077，NM_004881，NM_018494，NM_033285，NM_001197，NM_001540，NM_003820，NM_147780，NM_001003940，NM_000389，NM_016639，NM_000043，NM_002307，NM_021603，NM_004925，NM_000014，NM_022449，NM_005855，NM_006868， NM_032493，NM_007097，NM_005697，NM_004073，NM_182795，NM_005072，NM_144606，NM_014059，NM_002754，NM_003254，NM_001553，NM_003255，NM_013376，NM_000107，NM_0 04433，NM_021969，NM_023039，NM_080875，NM_002166，NM_001421，NM_153813，U68019，NM_015655，NM_031918，NM_001554，NM_003960，NM_032965，NM_201397，NM_005064，NM_005345，NM_001531，NM_006404，NM_002119，NM_005101，NM_006426，NM_020728，NM_016441，NM_203391，NM_006775， NM_052937，NM_003932，NM_030979，NM_003932，NM_030979，NM_003932，NM_030979，NM_032549，NM_007195，NM_002139，CO033021，NM_016093，CR611166，NM_017437，NM_033114，NM_000236，NM_174936，NM_004375，NM_004685，NM_052965，NM_201278，NM_003093，AV757313，NM_014791，BX537987，BC041925，BC032643，NM_016058，NM_181886， NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271，NM_016271， NM_020236，NM_001017430，NM_031157，NM_018981，NM_018291，NM_018048，NM_012207，NM_000028，NM_014321，NM_015423，NM_001034，AB032990，NM_000791，NM_199040，NM_006886，NM_004457，NM_007295，NM_006111 ，NM_001443，NM_000016，NM_001809，AL833119，NM_006493，NM_138271，NM_006136，CR615278，NM_024704，NM_003011，NM_203401，CR749233，AF277624，NM_013282，NM_198893，NM_013361，NM_002129，NM_002128，NM_003429，NM_003423，NM_016220，NM_022103，NM_021269，NM_016649，NM_199132 ，AK098175，NM_198381，NM_138330，NM_030824，NM_003441，NM_001001415，AK128731，NM_203282，CR627133，NM_007139，NM_178558，NM_024498，NM_003430，NM_003410，NM_178549，NM_152601，NM_024629，BC015987，NM_145233，NM_031942，NM_133473，NM_005655，NM_021994，NM_025189，NM_001008390 ，NM_024087，NM_032828，NM_006980，NM_024561，NM_173531，NM_003864，NM_012345，NM_003079，NM_006193，NM_003599，NM_006311，NM_020675，NM_018492，NM_145697，NM_006101，NM_002358，NM_006716，NM_003318，NM_018131，NM_012177，NM_013277，NM_018136，NM_014750，NM_016343，NM_017489 ，NM_006461，NM_001790，NM_004064，NM_080668，NM_021211，NM_001274，NM_001005414，NM_144710，NM_004336，NM_005496，NM_181803，NM_018101，AF147440，NM_005030，NM_022346，NM_019 084，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504，NM_004523，NM_005504， NM_018098，NM_004586，NM_002317，NM_031296，NM_014736，XM_292197，NM_016513，NM_001331，AB032991，NM_004232，NM_003472，NM_012334，NM_012425，NM_002140，NM_005271，NM_145203，BU679059，NM_006305，NM_020824，NM_012120，NM_012089，NM_021977，NM_015171，NM_016467，AA484677， BC035622，NM_002520，NM_014043，NM_003133，NM_013322，NM_005733，NM_014322，NM_020401，NM_138285，NM_001786，NM_001067，NM_001012271，NM_024854，NM_018204，NM_021999，NM_005400，NM_148957，NM_001006，NM_006437，NM_024055，NM_000582，NM_016951，NM_002737，NM_004836，NM_020686， NM_004134，NM_053039，NM_007195，NM_005256，NM_003368，NM_001018115，AI281523，NM_004075，NM_002389，NM_000584，NM_001801，NM_000715，NM_144503，NM_145697和NM_001010914中。
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公开(公告)号：KR100994996B1
公开(公告)日：2010-11-18
申请号：KR1020080076917
申请日：2008-08-06
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6876 , C12Q2600/158
Abstract: 본 발명은 페난트렌(phenanthrene)에 대한 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 구체적으로 다환 방향족 탄화수소류 중의 하나인 페난트렌에 의해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 페난트렌에 대한 노출 여부를 확인하는 방법에 관한 것으로, 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 페난트렌의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 페난트렌에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
페난트렌, 다환 방향족 탄화수소, 바이오마커, 마이크로 어레이-
Patent Agency Ranking
公开(公告)号：KR1020090090083A
公开(公告)日：2009-08-25
申请号：KR1020080015344
申请日：2008-02-20
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6837 , C12N15/1003 , C12Q1/6888 , C12Q2600/158
Abstract: A bio marker for identifying the exposure of naphthanlene is provided to monitor and determine the contamination of naphthalene through DNA microarray chip. A DNA microarray chip for identifying the exposure of naphthalene comprises oligonucleotide containing whole or part of biomarker gene sequence or its complementary molecule. A method for identifying the exposure of naphthalene comprises: a step of isolating RNA of somatic cell of test sample which is experimental group and somatic cell of control group; a step of synthesizing cDNA and probing with each different fluorescence; a step of hybridizing the cDNA and DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing the expression between control group and experimental group.
Abstract translation: 提供了用于鉴定萘醌暴露的生物标记物，用于通过DNA微阵列芯片监测和确定萘的污染。 用于鉴定萘暴露的DNA微阵列芯片包含含有全部或部分生物标记基因序列或其互补分子的寡核苷酸。 用于鉴定萘暴露的方法包括：分离作为实验组和对照组的体细胞的测试样品的体细胞的RNA的步骤; 合成cDNA并探索各种不同荧光的步骤; 将cDNA和DNA微阵列芯片杂交的步骤; 分析DNA微阵列芯片的步骤; 并比较对照组和实验组之间的表达。
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公开(公告)号：KR100985090B1
公开(公告)日：2010-10-04
申请号：KR1020080047946
申请日：2008-05-23
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q2600/142 , C12Q2600/158
Abstract: The present invention relates to a method for confirming the exposure on chrysene using a DNA fragment whose expression is increased or decreased specifically when it is exposed to chrysene. The method of the present invention is effective in determination of risk by chrysene and monitoring the chrysene exposure, so that it can be effectively used as a tool for examining the mechanism of chrysene induced toxicity.
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公开(公告)号：KR100968762B1
公开(公告)日：2010-07-08
申请号：KR1020080015344
申请日：2008-02-20
Applicant: 한국과학기술연구원
Abstract: 본 발명은 나프탈렌 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 구체적으로 다환 방향족 탄화수소류 중의 하나인 나프탈렌(Naphthalene)에 의해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 나프탈렌에 대한 노출 여부를 확인하는 방법에 관한 것이다. 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 나프탈렌의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 나프탈렌에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
나프탈렌(Naphthalene), 다환 방향족 탄화수소, 바이오마커, 마이크로 어레이-
公开(公告)号：KR1020100018237A
公开(公告)日：2010-02-17
申请号：KR1020080076917
申请日：2008-08-06
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6876 , C12Q2600/158 , C40B40/06
Abstract: PURPOSE: A biomarker for confirming phenanthrene exposure is provided to monitor and determine phenanthrene pollution and to find a toxicity mechanism. CONSTITUTION: A biomarker for confirming phenanthrene contains a gene of which an expression is increased or decreased due to exposure to phenanthrene. A DNA microarray chip for confirming phenanthrene exposure contains oligotide having an entire or partial gene sequence or its complementary oligonuecleotide. A method for detecting the biomarker for confirming phenanthrene exposure comprises: a step of isolating RNA from a test person's somatic cells as an experimental group and normal somatic cells as a control group; a step of synthesizing cDNA of the experimental group and control group and labeling with different fluorescences; a step of hybridizing the labeled cDNA on a microarray chip; a step of analyzing DNA microarray chip; and a step of comparing expression level between control group and experimental group.
Abstract translation: 目的：提供一种用于确定菲暴露的生物标志物，用于监测和确定菲污染并找到毒性机制。 构成：用于确认菲的生物标志物含有由于暴露于菲而表达增加或减少的基因。 用于确认菲暴露的DNA微阵列芯片含有具有全部或部分基因序列的寡核苷酸或其互补寡核苷酸。 用于检测用于确认菲暴露的生物标志物的方法包括：将作为实验组和正常体细胞作为对照组的来自测试人体细胞的RNA分离的步骤; 合成实验组和对照组的cDNA并用不同荧光标记的步骤; 将标记的cDNA杂交到微阵列芯片上的步骤; 分析DNA微阵列芯片的步骤; 比较对照组和实验组之间的表达水平比较。
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公开(公告)号：KR1020090121842A
公开(公告)日：2009-11-26
申请号：KR1020080047946
申请日：2008-05-23
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q2600/142 , C12Q2600/158 , C40B40/06
Abstract: PURPOSE: A biomarker for confirming exposure to chrysene is provided to monitor and determine chrysene contamination and study of toxic mechanism by the chrysene. CONSTITUTION: A method for detecting biomarker for confirming exposure of chrysene comprises: a step of isolating RNA from human somatic cell of sample as experimental group and somatic cell of control group; a step of synthesizing cDNA then hybridizing the experimental group and control group with different fluorescences; a step of hybridizing the cDNA on a DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing the expression level of biomarker between control group and experimental group.
Abstract translation: 目的：确定暴露于中国的生物标志物，用于监测和确定中华人民共和国污染物，并研究其毒性机制。 构成：检测生物标志物用于确认暴露的生物标志物的方法包括：将样品的人体细胞的RNA分离为对照组的实验组和体细胞的步骤; 合成cDNA的步骤，然后用实验组和对照组与不同的荧光素杂交; 将cDNA杂交到DNA微阵列芯片上的步骤; 分析DNA微阵列芯片的步骤; 比较对照组和实验组生物标志物的表达水平。
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