-
Patent Agency Ranking
-
公开(公告)号：KR1019970002667B1
公开(公告)日：1997-03-07
申请号：KR1019930021925
申请日：1993-10-21
Applicant: 한국과학기술연구원
Abstract: Disclosed is the mass production process of the peroxidase using anion exchange resin, which is possible to separate and produce the sweet-potato peroxidase in large quantities by culturing a sweet potato culture cell strain. The process comprises the following steps of; A callus is induced by cutting apical meristem from a new shoot of the potato, and then culturing it on a solid medium containing 2,4-dichlorophenoxy acetic acid. A cell strain having a high peroxidase activity and a good cell proliferation in the induced callus is separated to obtain the mass producing cell strain by culturing it again on the same medium. The mass producing cell strain, which can produce the peroxidase, is cultured in a nutrient medium under a suitable suspension condition. An ethylene or abscisic acid are added to a culture fluid on the way of cultivation, wherein improving the peroxidase productivity remarkably.
Abstract translation: 公开了使用阴离子交换树脂的过氧化物酶的大量生产方法，其可以通过培养甘薯培养细胞株分离并产生大量的甘薯过氧化物酶。 该过程包括以下步骤： 通过从马铃薯的新芽切割顶端分生组织诱导愈伤组织，然后在含有2,4-二氯苯氧乙酸的固体培养基上培养。 分离出在诱导愈伤组织中具有高过氧化物酶活性和良好细胞增殖的细胞株，通过在同一培养基上再次培养来获得批量生产细胞株。 可以在合适的悬浮条件下在营养培养基中培养可产生过氧化物酶的大量生产细胞株。 在培养途中向培养液中加入乙烯或脱落酸，其中显着提高过氧化物酶的生产。
-
公开(公告)号：KR100314905B1
公开(公告)日：2002-10-31
申请号：KR1019980060442
申请日：1998-12-29
Applicant: 한국과학기술연구원
IPC: C12N1/00
Abstract: 본 발명은 미생물을 이용하여 리그닌 분해효소의 일종인 락케이즈 (laccase)를 고수율로 생산하는 방법에 관한 것으로, 보다 상세하게는 백색 부후균 (white rot fungi)을 배양할 때 배양액에 에탄올, 메탄올, 또는 이소프로필 알콜 등의 알콜류 중 어느 하나 혹은 하나 이상을 유도물질로 첨가함으로써 락케이즈의 생산을 증대시키는 방법으로서, 본 발명의 방법은 값싸고 인체에 무해한 알콜을 사용하므로 경제적이고 환경보호의 측면에서도 바람직하다.
-
公开(公告)号：KR1020000043999A
公开(公告)日：2000-07-15
申请号：KR1019980060442
申请日：1998-12-29
Applicant: 한국과학기술연구원
IPC: C12N1/00
Abstract: PURPOSE: Mass production method of laccase, which is a kind of ligninase, by fermentation of fungi is provided which uses nontoxic and cheap inducers instead of toxic and expensive aromatic ones. CONSTITUTION: White rot fungi such as Trametes versicolar, Coriolus hirsutus, Phanerochaete flavido-alba, Grifila frondosa, Phlebia radiata, Pycnoporos cinnabarinus and Botrytis cineria are tried to produce laccase. White rot fungus is inoculated in a the medium and pH of the medium is adjusted to 5.5-6.5. Laccases are obtained from the broth after one week cultivation, aerated and agitated properly. Addition of alcohol into the medium increases the production of laccase depending on the volume of alcohol being added. The activity of laccase is increased until the volume of alcohol reaches to 0.5-4.0 volume percent of broth but it is dropped quickly at 5.0 volume percent. Aliphatic alcohols such as methanol, ethanol or isopropylalcohol are used to increase the production of laccase. Addition of 2 % of isopropyl alcohol, methanol and ethanol in the medium show 4 times, 9.5 times and 15.6 times laccase activity respectively.
Abstract translation: 目的：提供通过发酵真菌的木质素酶的大量生产方法，其使用无毒廉价的诱导剂，而不是有毒和昂贵的芳族化合物。 构成：尝试产生漆酶的白腐真菌，例如冬虫夏草，Coriolus hirsutus，Phanerochaete flavido-alba，Grifila frondosa，Phlebia radiata，Pycnoporos cinnabarinus和Botrytis cineria。 将白腐菌接种在培养基中，将培养基的pH调节至5.5-6.5。 经培养一周后，从肉汤获得漆酶，充分充气并充分搅拌。 将醇加入培养基中，根据加入的酒精体积增加漆酶的产量。 漆酶的活性增加，直到醇的体积达到肉汤的0.5-4.0体积百分数，但是其以5.0体积％快速下降。 使用脂肪族醇如甲醇，乙醇或异丙醇来增加漆酶的产生。 在培养基中加入2％异丙醇，甲醇和乙醇分别显示漆酶活性的4倍，9.5倍和15.6倍。
-
公开(公告)号：KR1019950011612A
公开(公告)日：1995-05-15
申请号：KR1019930021925
申请日：1993-10-21
Applicant: 한국과학기술연구원
Abstract: 본 발명은 음이온 교환 수지를 사용하여 분리가능한 신규의 고구마 유래 퍼옥시다제, 상기 퍼옥시다제를 효율적으로 대량 생산할 수 있는 고구마 배양세포주, 상기 배양세포주를 배양하여 퍼옥시다제를 대량 생산하는 방법 및 상기 방법으로 생산된 퍼옥시다제를 배양세포로부터 효과적으로 정제하는 방법에 관한 것이다.
-
-
-
-
Search Results
5
records
(took 0.017 seconds)
