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公开(公告)号：KR100963315B1
公开(公告)日：2010-06-14
申请号：KR1020080012356
申请日：2008-02-11
Applicant: 한국과학기술연구원
CPC classification number: C12N9/001 , C07K2299/00 , C12Y103/01009 , G01N2333/90206 , G01N2500/20
Abstract: 본 발명은
Thermotoga
maritima 균주로부터 유래하는 FabK (enoyl-acyl carrier protein reductase) 단백질에 관한 것으로, 상기 단백질의 활성 부위와 3차원 결정 구조를 규명하여, 이를 이용하여 신규한 FabK 단백질 저해물질을 스크리닝하여, 기존의 항생제에 내성을 보이는 균주에 대해서 우수한 항균 활성을 보이는 신규 활성 조절 물질을 개발하는 기술에 관한 것이다.-
公开(公告)号：KR1020090072904A
公开(公告)日：2009-07-02
申请号：KR1020080012356
申请日：2008-02-11
Applicant: 한국과학기술연구원
CPC classification number: C12N9/001 , C07K2299/00 , C12Y103/01009 , G01N2333/90206 , G01N2500/20
Abstract: A method for developing a FabK inhibitor is provided to screen a FabK inhibiting agent using active fragment of FabK and three dimensional structure. A method for crystallizing a FabK protein comprises a step of containing FabK protein solution with container solution comprising buffer solution, metal salt and precipitator. The concentration of FabK protein solution is 3-30mg/ml. The pH concentration and concentration is 4-9.5 and 0.005-1.5M. The metal salt is alkaline metal salt and alkaline metal salt. A precipitator is selected from alcohol of C1-4, and the concentration in the container solution is 1-40%(v/v). FabK-FMN(falvin mononucleotide) complex crystalline has three dimensional structure. A method for developing an inhibitor of FabK inhibitor is to use the three dimensional crystalline structure information.
Abstract translation: 提供用于开发FabK抑制剂的方法，以使用FabK的活性片段和三维结构筛选FabK抑制剂。 用于结晶FabK蛋白的方法包括含有包含缓冲溶液，金属盐和除尘器的容器溶液的FabK蛋白溶液的步骤。 FabK蛋白溶液的浓度为3-30mg / ml。 pH浓度为4-9.5和0.005-1.5M。 金属盐是碱金属盐和碱金属盐。 沉淀器选自C1-4的醇，容器溶液中的浓度为1-40％（v / v）。 FabK-FMN（falvin单核苷酸）复合结晶具有三维结构。 开发FabK抑制剂抑制剂的方法是使用三维晶体结构信息。
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