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    2.
    发明授权

    公开(公告)号:KR1019920001374B1

    公开(公告)日:1992-02-11

    申请号:KR1019890003676

    申请日:1989-03-23

    Abstract: A cellulase producing clostridium sp. strain is screened by (a) mixing sample, e.g. waste water of cowshed, with cellulose-contg. medium, (b) heating the mixt. at 80 deg.C for 15 mm. and culturing, (c) innoculating the cultured broth in the medium contg. 0.1-0.2 % glucose, cellulose and agar, and (d) culturing it for 7-10 days and selecting the straing having a strong cellulase activity, clostridium sp. SC221 (KCTC 8440) (I). The spore of (I) is obtd. by culturing in the medium contg. 0.5 % cellobilose, 1.0 % glucose, 1.0 % cellulose, 0.5 % peptone, 0.5 % trypticase, mineral etc.. The obtd (I) or spore of (I) is useful for feed additive.

    Abstract translation: 生产梭菌的纤维素酶 通过以下步骤筛选菌株:(a)混合样品,例如 牛舍废水,用纤维素 介质,(b)加热混合物。 在80℃下15mm。 并培养,(c)在培养基中接种培养的肉汤。 0.1-0.2%葡萄糖,纤维素和琼脂,(d)培养7-10天,选择具有强纤维素酶活性的梭菌(Clostridium sp。)。 SC221(KCTC 8440)(I)。 (I)的孢子是有效的。 通过在培养基中培养 0.5%纤维二糖,1.0%葡萄糖,1.0%纤维素,0.5%蛋白胨,0.5%胰蛋白酶,矿物质等。(Ⅰ)或(I)的孢子可用于饲料添加剂。

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