Abstract:
본 발명은 TESK1 억제제 또는 TESK1에 의해 매개되는 코필린 인산화 억제제를 유효성분으로 함유하는 항암제 내성 억제용 조성물에 관한 것으로, 본 발명에 따른 항암제 내성 억제용 조성물은 항암제의 투여에 따라 발생하는 액틴 리모델링을 억제하여, 암세포가 항암제에 대해 저항성을 가지는 것을 억제하여, 항암제 내성 억제에 유용하다.
Abstract:
A method for selecting microorganisms cloned with a target gene by using photochemical reaction is provided to remove undesired microorganism clones using simple photo illumination prior to the desired gene screening, so that the gene cloned with a desired gene fragment or its library is able to be simply screened. The method for selecting microorganisms cloned with a target gene by using photochemical reaction comprises the steps of: preparing a recombinant vector by inserting a target gene or its library and a promoter of the target gene into a recombinant plasmid containing origin, antibiotic makers, restriction enzyme sites and ferrochelatase gene(hemH); transforming a microorganism with the recombinant vector; culturing the transformed microorganism in a medium containing antibiotics with photo illumination; and validating the cloning of microorganism when the transformed microorganism is grown, wherein the transformed microorganism is obtained by inserting the ferrochelatase gene(hemH) into a photosensitive mutant microorganism obtained by deleting the ferrochelatase gene(hemH); the recombinant plasmid is p19hemH; and the microorganism is Escherichia coli.