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公开(公告)号：KR1020130123688A
公开(公告)日：2013-11-13
申请号：KR1020120047005
申请日：2012-05-03
Applicant: 한국과학기술원
Abstract: The present invention relates to a method for screening extracellularly secreted transcription factors among homeodomain transcription factors. More particularly, the method is used for selecting transcription factors considering the proper secretion amount according to the use of homeodomain transcription factors by introducing 105 human homeodomain transcription factors to each vector, preparing a vector library, transforming the vectors into 293T cells, analyzing the quantity of proteins secreted to a culture medium and the quantity of proteins remaining in cells, determining extracellularly secreted homeodomain transcription factors, and confirming relative secretion ratio through comparing the quantity of the secreted homeodomain transcription factors with the quantity of a positive control group.
Abstract translation: 本发明涉及在同源结构域转录因子中筛选细胞外分泌的转录因子的方法。 更具体地说，根据同位素域转录因子的使用，考虑到适当的分泌量，通过将105个同源异构域转录因子引入每个载体，制备载体文库，将载体转化成293T细胞，分析数量 分泌到培养基中的蛋白质和细胞中剩余的蛋白质的量，确定细胞外分泌的同位素转录因子，并通过比较分泌的同位素域转录因子的量与阳性对照组的量来确认相对分泌比。
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公开(公告)号：KR101542139B1
公开(公告)日：2015-08-05
申请号：KR1020120047005
申请日：2012-05-03
Applicant: 한국과학기술원
Abstract: 본발명은호메오도메인전사인자들중 세포외로분비가능한전사인자를스크리닝하는방법에관한것으로, 더욱구체적으로는인간호메오도메인전사인자 105개를벡터에각각도입하여벡터라이브러리를제작하였으며, 각각의벡터들을 293T 세포에형질전환한뒤 배양배지로분비된단백질과세포내에남아있는단백질의양을분석하여세포외로분비되는호메오도메인전사인자를결정하였으며, 분비되는호메오도메인전사인자의양을양성대조군과비교하여상대적분비비율을확인하였으므로, 호메오도메인전사인자의용도에따른적정분비량을고려한전사인자의선택에유용하게사용될수 있다.
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公开(公告)号：KR101456998B1
公开(公告)日：2014-11-05
申请号：KR1020120040983
申请日：2012-04-19
Applicant: 한국과학기술원
IPC: C12N15/62 , C12N15/867 , C12N5/10 , C12N5/0735
Abstract: 본 발명은 역분화 줄기세포 유도인자의 세포 내 도입에 호메오 도메인의 단백질 분비 아미노산 서열을 이용하여 고효율의 비유전자 변형에 의한 역분화 줄기세포를 제조하는 방법에 관한 것으로서, 세포 외부로 분비되어 주변 세포 내부로 이동할 수 있는 분비-침투 관련 부위와 역분화 유도인자의 융합단백질을 발현하는 형질전환세포를 제조하여 이를 분화된 대상 체세포와 공동 배양함으로써 상기 형질전환세포로부터 분비되는 역분화 유도인자에 의해 상기 대상 체세포로부터 역분화 줄기세포를 제조하였으므로, 본 발명의 상기 융합단백질을 암호화하는 유전자 컨스트럭트 및 이를 발현하는 형질전환세포는 분화된 체세포로부터 미분화의 역분화 줄기세포를 제조하는데 유용하게 사용될 수 있다.
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公开(公告)号：KR1020130118080A
公开(公告)日：2013-10-29
申请号：KR1020120040983
申请日：2012-04-19
Applicant: 한국과학기술원
IPC: C12N15/62 , C12N15/867 , C12N5/10 , C12N5/0735
Abstract: PURPOSE: A method for preparing induced pluripotent stem cells using intracellular protein transduction is provided to effectively prepare induced pluripotent stem cells from differentiated cells by protein transduction with genetic safety and high efficiency. CONSTITUTION: A gene construct contains a fusion gene containing a gene with a base sequence of sequence number 1 or 2 and a gene encoding dedifferentiation-inducing factors. The gene construct is operatively linked to a recombinant expression vector. A transformant is prepared by transducing the recombinant expression vector into host cells. A method for preparing the induced pluripotent stem cells from differentiated cells comprises the steps of: mixing the transformant or a culture liquid thereof with differentiated cells and co-culturing the mixture; and isolating embryonic stem cell-like colonies derived from the differentiated cells.
Abstract translation: 目的：提供使用细胞内蛋白转导制备诱导性多能干细胞的方法，通过遗传安全性和高效率的蛋白质转导，有效地从分化细胞中制备诱导的多能干细胞。 构成：基因构建体含有包含具有序列号1或2的碱基序列的基因和编码去分化诱导因子的基因的融合基因。 该基因构建体与重组表达载体可操作地连接。 通过将重组表达载体转导到宿主细胞中制备转化体。 从分化细胞制备诱导多能干细胞的方法包括以下步骤：将转化体或其培养液与分化细胞混合并共培养混合物; 并分离衍生自分化细胞的胚胎干细胞样集落。
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