公开(公告)号：KR1020010033130A

公开(公告)日：2001-04-25

申请号：KR1020007006503

申请日：1998-12-15

Applicant: 한국과학기술원

Inventor： 강창원 ,  권영수 ,  김영태 ,  쾨스터후베르트 ,  리틀다니엘피 ,  지앙구오빙 ,  러프데이비드엠 ,  캔터챨스 ,  오'도넬매리안제이

IPC: C12Q1/68

CPC classification number: C12Q1/6872 ,  B01J2219/00317 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  C12Q2525/143

Abstract: PURPOSE: A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods generate a set of nested RNA transcripts, which are analyzed mass spectrometry, using the RNA polymerase. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided. CONSTITUTION: A method for determining the sequence of a target nucleic acid molecule, comprising: a) providing a nucleic acid molecule comprising a promoter and target nucleic acid sequence operatively linked to the promoter, wherein the nucleic acid molecule is immobilized on a solid support; b) transcribing the promoter-containing nucleic acid molecule with an RNA polymerase that recognizes the promoter under conditions whereby a nested set of RNA transcripts from the target is produced; and c) determining the molecular weight values of the transcripts by mass spectrometry and thereby determining the nucleic acid sequence of the target molecule.

Abstract translation: 目的：提供使用RNA聚合酶测序核酸的质谱法，包括DNA依赖性和RNA依赖性RNA聚合酶。 该方法产生一组嵌套的RNA转录物，其使用RNA聚合酶进行质谱分析。 还提供了鉴定转录终止子序列或衰减子序列的方法。 构成：确定靶核酸分子序列的方法，包括：a）提供包含可操作地与启动子连接的启动子和靶核酸序列的核酸分子，其中所述核酸分子被固定在固体支持物上; b）使用识别启动子的RNA聚合酶转录含有启动子的核酸分子，由此产生来自目标的嵌套RNA转录物组; 和c）通过质谱确定转录物的分子量值，从而确定靶分子的核酸序列。

公开(公告)号：KR100463459B1

公开(公告)日：2004-12-29

申请号：KR1020007006503

申请日：1998-12-15

Applicant: 한국과학기술원

Inventor： 강창원 ,  권영수 ,  김영태 ,  쾨스터후베르트 ,  리틀다니엘피 ,  지앙구오빙 ,  러프데이비드엠 ,  캔터챨스 ,  오'도넬매리안제이

IPC: C12Q1/68

CPC classification number: C12Q1/6872 ,  B01J2219/00317 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  C12Q2525/143

Abstract: A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.

Abstract translation: 提供了使用RNA聚合酶（包括DNA依赖性和RNA依赖性RNA聚合酶）对核酸进行测序的质谱方法。 该方法使用经修改的Sanger测序策略，其中使用RNA聚合酶来产生通过碱基特异性链终止获得的一组巢式RNA转录物。 这些通过质谱分析。 还提供了鉴定转录终止子序列或衰减子序列的方法。