公开(公告)号：KR1020100067298A

公开(公告)日：2010-06-21

申请号：KR1020080125787

申请日：2008-12-11

Applicant: 한국과학기술원

Inventor： 신현정 ,  윤재영 ,  김순희

IPC: C12M1/26 ,  C12M1/32 ,  C12M1/00

CPC classification number: C12M35/04

Abstract: PURPOSE: A cell stimulator and culturing apparatus are provided to obtain enough cells which are need for cell analysis by stimulating cell directly and mechanically. CONSTITUTION: A cell stimulator comprises a PDMS(polymethylsiloxane film(104)), and plural microneedle(101) which is integrally formed with the PDMS(polydimethylsiloxane) film and has predetermined interval in a vertical direction. The microneedle is formed of PDMS, silicon, or PMMA. The cell stimulator further comprises a microneedle support(102) and a spacer(103) formed between the microneedle support and PDMS film. An apparatus for culturing and cell stimulation comprises: the cell stimulator; a reaction chamber which is divided into a upper portion and lower portion based on the PDMS film; a pneumatic application unit which applies air pressure to the upper area of the reaction chamber to expand the PDMS film; a cell culture device which is separately placed from the microneedle in a predetermined interval; and a circulation unit for cell culture medium.

Abstract translation: 目的：提供细胞刺激器和培养装置，以通过直接和机械刺激细胞获得足够的细胞，这些细胞需要细胞分析。 构成：细胞刺激器包括PDMS（聚甲基硅氧烷膜（104））和与PDMS（聚二甲基硅氧烷）膜一体形成并且在垂直方向上具有预定间隔的多个微针（101）。 微针由PDMS，硅或PMMA形成。 细胞刺激器还包括形成在微针支架和PDMS膜之间的微针支撑（102）和间隔物（103）。 培养和细胞刺激的装置包括：细胞刺激剂; 反应室，其基于PDMS膜分为上部和下部; 将空气压力施加到反应室的上部区域以膨胀PDMS膜的气动施加单元; 细胞培养装置，其以预定间隔从所述微针分离放置; 和细胞培养基的循环装置。