公开(公告)号：KR100377946B1

公开(公告)日：2003-03-29

申请号：KR1020000040829

申请日：2000-07-15

Applicant: 한국과학기술원

Inventor： 김학성 ,  윤현철 ,  홍미영

IPC: C12Q1/68

CPC classification number: B82Y15/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/0072 ,  B01J2219/00725 ,  B82Y5/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07K16/00 ,  C40B40/10 ,  C40B60/14

Abstract: The present invention relates to a process for preparing monolayers and microarrays of biomolecules by reacting functionalized dendrimers on a solid surface with biomolecules such as proteins, antigens, antibodies, enzymes, ligands, receptors, and the like. The present invention can be widely applied to the areas including preparation of kits and biosensors for disease diagnosis and compound analyses using the ascribed biomolecules as target substances, and more recently, integrated high-throughput analyzing system such as development of protein chips.

Abstract translation: 本发明涉及通过使固体表面上的官能化树枝状聚合物与生物分子如蛋白质，抗原，抗体，酶，配体，受体等反应来制备生物分子的单层和微阵列的方法。 本发明可以广泛地应用于包括用于疾病诊断和使用归属的生物分子作为目标物质的化合物分析的试剂盒和生物传感器的制备，以及最近诸如蛋白质芯片的开发的集成高通量分析系统。

公开(公告)号：KR1020020007083A

公开(公告)日：2002-01-26

申请号：KR1020000040829

申请日：2000-07-15

Applicant: 한국과학기술원

Inventor： 김학성 ,  윤현철 ,  홍미영

IPC: C12Q1/68

CPC classification number: B82Y15/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/0072 ,  B01J2219/00725 ,  B82Y5/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07K16/00 ,  C40B40/10 ,  C40B60/14

Abstract: PURPOSE: A process for producing monolayer using dendrimer is provided, therewith proteins, antigens, antibodies, enzymes, receptors and ligands are reacted to produce biomolecular monolayer. CONSTITUTION: The process for producing monolayer using dendrimer comprises the steps of: obtaining self-assembled monolayer by binding alkanethiol having amine or succinimide at one of its end, on the surface of metal or glass; and reacting dendrimer containing amine or carboxyl having N-hydroxysuccinimide, in which the alkanethiol is dithiopropionic acid bis-N-hydroxy succinimide ester or cystamine dihydrochloride; and the dendrimer is selected from the group consisting of dendrimers consisting of G1, G2, G3, G4 and G5 having amine, and dendrimers consisting of G1.5, G2.5, G3.5, G4.5 and G5.5, wherein carboxyl group at its end has N-hydroxysuccinimide.

Abstract translation: 目的：提供使用树枝状聚合物制备单层的方法，其中蛋白质，抗原，抗体，酶，受体和配体反应产生生物分子单层。 构成：使用树枝状聚合物制备单层的方法包括以下步骤：通过在其一端，金属或玻璃表面上结合具有胺或琥珀酰亚胺的链烷硫醇来获得自组装单层; 并且使含有N-羟基琥珀酰亚胺的含有胺或羧基的树枝状聚合物反应，其中链烷硫醇是二硫代丙酸双-N-羟基琥珀酰亚胺酯或二胺盐胺; 并且树枝状大分子选自由具有胺的G1，G2，G3，G4和G5组成的树枝状大分子，以及由G1.5，G2.5，G3.5，G4.5和G5.5组成的树枝状大分子，其中 其末端的羧基具有N-羟基琥珀酰亚胺。