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公开(公告)号：KR1020210046205A
公开(公告)日：2021-04-28
申请号：KR1020190129684
申请日：2019-10-18
Applicant: 한국과학기술원
Abstract: 본발명은 folE 유전자또는 ribF 유전자가넉아웃(kmock out) 되거나, folE 유전자및 ribF 유전자가넉아웃(kmock out)된리보플라빈생성능이증가된재조합류코노스톡시트륨균주및 상기재조합류코노스톡시트륨균주를이용한리보플라빈의제조방법에관한것이다.
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公开(公告)号：KR1020130123770A
公开(公告)日：2013-11-13
申请号：KR1020120047152
申请日：2012-05-03
Applicant: 한국과학기술원
IPC: G01N33/533 , G01N33/574 , G01N33/68 , G01N33/52
CPC classification number: G01N33/533 , G01N33/52 , G01N33/574 , G01N33/6821 , G01N33/6824
Abstract: The present invention provides a kit for fluoroimmunoassay, comprising a solid support which is conjugated with a target molecule; and a detection reagent containing kringle domain variant with specific binding capacity to the target molecule and a fluorescent substance-conjugated material. Also, the present invention provides a method for diagnosing cancer using the conjugate by fluorescence-linked immunosorbent assay (FLISA). According to the present invention, the method is useful for effectively and cheaply diagnosing diseases and is more simple than an existing method using a kringle domain variant which is a non-antibody protein skeleton effectively binding to the target protein with high solubility and productivity instead of an antibody and the fluorescent substance-conjugated material. [Reference numerals] (AA) Competitive enzymelinked immunosorbent assay;(BB) Competitive fluorescence immunosorbent assay;(CC) Colorimetric reaction;(DD,GG) Being washed out;(EE,HH,MM,PP) Target molecular;(FF,II) Actual specimen;(JJ) Second reaction;(KK) Fluorescence detection;(LL,OO) Recombinant protein;(NN,QQ) First reaction
Abstract translation: 本发明提供了用于荧光免疫测定的试剂盒，其包含与靶分子缀合的固体支持物; 以及含有对靶分子具有特异结合能力的三环结构域变体和荧光物质共轭材料的检测试剂。 另外，本发明提供了一种通过荧光连接免疫吸附测定法（FLISA）使用该结合物诊断癌症的方法。 根据本发明，该方法对于有效和廉价地诊断疾病是有用的，并且比使用作为非抗体蛋白骨架的具有高度溶解性和生产力的非抗体蛋白质骨架有效结合的三环结构域变体的现有方法更为简单，而不是 抗体和荧光物质共轭材料。 （AA）竞争性酶联免疫吸附测定;（BB）竞争荧光免疫吸附测定;（CC）比色反应;（DD，GG）被洗出;（EE，HH，MM，PP）靶分子;（FF， II）实际标本;（JJ）第二反应;（KK）荧光检测;（LL，OO）重组蛋白;（NN，QQ）第一反应
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公开(公告)号：KR102247462B1
公开(公告)日：2021-05-03
申请号：KR1020190129684
申请日：2019-10-18
Applicant: 한국과학기술원
Abstract: 본발명은 folE 유전자또는 ribF 유전자가넉아웃(kmock out) 되거나, folE 유전자및 ribF 유전자가넉아웃(kmock out)된리보플라빈생성능이증가된재조합류코노스톡시트륨균주및 상기재조합류코노스톡시트륨균주를이용한리보플라빈의제조방법에관한것이다.
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公开(公告)号：KR101809135B1
公开(公告)日：2017-12-15
申请号：KR1020120047152
申请日：2012-05-03
Applicant: 한국과학기술원
IPC: G01N33/533 , G01N33/574 , G01N33/68 , G01N33/52
Abstract: 본발명은표적분자가결합되어있는고체지지체; 및상기표적분자에특이적결합능을갖는크링글도메인변이체와형광물질의결합물을함유하는검출시약을포함하는형광면역측정용키트를제공한다. 또한, 상기결합물을이용하여형광면역분석 (FLISA)법으로암을진단하는방법을제공한다. 본발명에따르면, 항체를대신하여용해성및 생산성이높으며표적분자에효과적으로결합하는비항체단백질골격인크링글도메인변이체와형광물질을결합시킨결합물을이용하는바기존방법에비해더욱간단하면서저비용으로질병을효과적으로진단할수 있으므로유용하다.
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