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公开(公告)号:WO2015009074A2
公开(公告)日:2015-01-22
申请号:PCT/KR2014/006490
申请日:2014-07-17
Applicant: 씨제이제일제당 (주) , 한국과학기술원
CPC classification number: C12P13/001 , C12N9/88 , C12Y401/01017
Abstract: 본 발명은 향상된 퓨트레신 생산능을 갖는 신규한 변이 오르니틴 디카복실레이즈 단백질 및 이의 용도에 관한 것이다.
Abstract translation: 本发明涉及具有改进的腐胺生产力的新型突变鸟氨酸脱羧酶蛋白及其用途。
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2.
公开(公告)号:KR101835164B1
公开(公告)日:2018-03-06
申请号:KR1020130084409
申请日:2013-07-17
Applicant: 씨제이제일제당 (주) , 한국과학기술원
CPC classification number: C12P13/001 , C12N9/88 , C12Y401/01017
Abstract: 본발명은신규한향상된퓨트레신생산능을갖는변이된오르니틴디카복실레이즈단백질및 이의용도에관한것이다. 본발명에따른변이된오르니틴디카복실라제단백질은천연형에비하여퓨트레신전환활성이 21배까지증가될뿐만아니라, 기존퓨트레신생산균주에도입시에퓨트레신생산활성을확연히증대시키는바, 기존의화학합성경로의대안으로보다효율적인퓨트레신의대량생산에널리활용될수 있을것이다.
Abstract translation: 本发明涉及一种具有新增强的增肌能力的新型鸟氨酸脱羧酶蛋白及其用途。 根据本发明不仅比野生型增加至21倍的腐胺转化活性的鸟氨酸脱羧酶的二蛋白突变,在传统的腐胺生产菌株栏显著增加入口epyu托盘新的生产活动 作为现有化学合成途径的替代品,它可以广泛用于大量生产更有效的腐胺。
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公开(公告)号:KR1020080063936A
公开(公告)日:2008-07-08
申请号:KR1020070000554
申请日:2007-01-03
Applicant: 한국과학기술원
Abstract: A portable translator is provided to offer the most necessary function to users wanting translation by enable the user to carry and hold the portable device easily with a slim size, and offering real-time translation, recording, and repeated listening functions. A microphone(110) receives language to be translated in a voice signal. An ADC(Analog/Digital Converter)(114) converts the received voice signal into a digital voice signal. A language converting/controlling unit converts the digital voice signal into the translated language having the same meaning as the inputted language and performs control needed for operating a portable translator. A translated language storage unit stores the translated language. An earphone and a speaker(132,134) output the translated language stored in the translation language storage unit to the outside in voice. A player repeatedly plays the stored translated language to the earphone/speaker depending on user selection. A switch(140) discriminates a translation/storage process, a playback process, and a stop process. An operation display unit(116) displays an operation process discriminated by the switch.
Abstract translation: 提供便携式翻译器,通过使用户能够轻松地携带和保持便携式设备,并提供实时的翻译,记录和重复聆听功能,为希望翻译的用户提供最必要的功能。 麦克风(110)在语音信号中接收要翻译的语言。 ADC(模拟/数字转换器)(114)将所接收的语音信号转换为数字语音信号。 语言转换/控制单元将数字语音信号转换为具有与输入语言相同含义的翻译语言,并执行操作便携式翻译器所需的控制。 翻译的语言存储单元存储翻译的语言。 耳机和扬声器(132,134)将存储在翻译语言存储单元中的翻译语言以语音输出到外部。 播放器根据用户选择重复播放存储的翻译语言给耳机/扬声器。 开关(140)识别转换/存储处理,重放处理和停止处理。 操作显示单元(116)显示由开关识别的操作处理。
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4.
公开(公告)号:KR1020160003615A
公开(公告)日:2016-01-11
申请号:KR1020150185185
申请日:2015-12-23
Applicant: 씨제이제일제당 (주) , 한국과학기술원
Abstract: 본발명은신규한향상된퓨트레신생산능을갖는변이된오르니틴디카복실레이즈단백질및 이의용도에관한것이다. 본발명에따른변이된오르니틴디카복실라제단백질은천연형에비하여퓨트레신전환활성이 21배까지증가될뿐만아니라, 기존퓨트레신생산균주에도입시에퓨트레신생산활성을확연히증대시키는바, 기존의화학합성경로의대안으로보다효율적인퓨트레신의대량생산에널리활용될수 있을것이다.
Abstract translation: 本发明涉及一种具有改进的腐胺生产力的新型突变鸟氨酸脱羧酶蛋白及其应用。 根据本发明,与天然型相比,通过将腐胺转化活性提高21倍,突变鸟氨酸脱羧酶蛋白可广泛用于高效批量生产腐胺,作为现有化学合成途径的替代物,并显着增加 腐胺生产活动被引入现有的腐胺生产菌株时。
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5.
公开(公告)号:KR1020150009890A
公开(公告)日:2015-01-27
申请号:KR1020130084409
申请日:2013-07-17
Applicant: 씨제이제일제당 (주) , 한국과학기술원
CPC classification number: C12P13/001 , C12N9/88 , C12Y401/01017
Abstract: The present invention relates to novel mutated ornithine decarboxylase protein having enhanced capability of producing purtrescine, and to use thereof. According to the present invention, the mutated ornithine decarboxylase protein has putrescine conversion activity 21 times greater than that of natural type protein. When introduced into a prior putrescine-producing strain, the mutated ornithine decarboxylase protein significantly increases putrescine producing activity of the strain. Therefore, the mutated ornithine decarboxylase protein is an alternative for a protein used in the prior chemical synthesis pathway, and is expected to be used for more efficient mass-production of putrescine. In the mutated ornithine decarboxylase protein, a 163rd isoleucine amino acid residue and a 165th glutamic acid from an N-terminal of ornithine decarboxylase (ODC) having an amino acid sequence represented by SEQ ID: 1 are mutated.
Abstract translation: 本发明涉及具有增强的产生purtresine的能力的新的突变鸟氨酸脱羧酶蛋白及其应用。 根据本发明,突变的鸟氨酸脱羧酶蛋白质具有比天然型蛋白质高21倍的腐胺转化活性。 当引入现有的腐胺生产菌株时,突变的鸟氨酸脱羧酶蛋白显着增加菌株的腐胺产生活性。 因此,突变的鸟氨酸脱羧酶蛋白是用于现有化学合成途径中的蛋白质的替代物,并且预期用于更有效地批量生产腐胺。 在突变的鸟氨酸脱羧酶蛋白中,具有SEQ ID:1所示氨基酸序列的鸟氨酸脱羧酶(ODC)N末端的第163位异亮氨酸氨基酸残基和第165位谷氨酸被突变。
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