公开(公告)号：KR101407234B1

公开(公告)日：2014-06-13

申请号：KR1020130151721

申请日：2013-12-06

Applicant: 한국기초과학지원연구원

Inventor： 정현석 ,  송치홍 ,  현재경 ,  전상미 ,  유동주 ,  박종필

IPC: H01J37/20 ,  H01J37/26

Abstract: The present invention relates to a liquid specimen holder of an electron microscope and an electron microscope using the same. According to the present invention, to solve problems of an existing electron microscope, for examples, the inconvenience of solidification and crystallization of a corresponding sample in a vacuum to monitor the liquid sample, the difficulty of monitoring the real image of the liquid sample in a liquid state due to the deformation of the sample, and the preparation of each sample according to a new condition whenever an experimental condition is changed, provided are the liquid sample holder of the electron microscope which protects a sample from vacuum and monitors the sample in a liquid state in real time and replaces a different experiment solution and injects an experiment solution such as culture fluid when the sample is mounted, thereby continuously monitoring the sample, and the electron microscope using the same.

Abstract translation: 本发明涉及一种电子显微镜的液体样本保持器和使用其的电子显微镜。 根据本发明，为了解决现有的电子显微镜的问题，例如，在真空中凝固和结晶相关样品以监测液体样品的不便之处，难以监测液体样品的真实图像 液体状态由于样品的变形，每当实验条件改变时，根据新的条件制备每个样品，提供了保护样品免受真空的电子显微镜的液体样品架，并在 液体状态，替代不同的实验溶液，并在样品安装时注入培养液等实验溶液，从而连续监测样品，使用电子显微镜。

公开(公告)号：KR101396420B1

公开(公告)日：2014-05-20

申请号：KR1020130151742

申请日：2013-12-06

Applicant: 한국기초과학지원연구원

Inventor： 정현석 ,  전상미 ,  현재경 ,  유동주 ,  송치홍 ,  박종필

IPC: G01N1/42 ,  G01N23/225

Abstract: The present invention relates to an apparatus for preparing a specimen containing a cryo stage for installing a grid cooled by liquefied nitrogen on a fluorescence microscope. The present invention provides an apparatus for preparing a correlative cryogenic specimen of an electron microscope of a novel structure which is formed in order to consecutively perform a pre-process of cooling the grid by inserting the grid into liquefied nitrogen and a series of processes of installing the grid on the fluorescence microscope by transferring the pre-processed grid and observing the grid within a single apparatus of preparing a specimen by solving the problems of an existing electron microscope in which the damage or breakage of the grid in a pre-processing of cooling the grid and a process of transferring the pre-processed grid and observing the grid is frequently generated, and a transporting operation of the grid is difficult and inconvenient since a temperature is constantly maintained during transferring the pre-processed grid.

Abstract translation: 本发明涉及一种用于制备含有用于在荧光显微镜上安装由液氮冷却的格栅的低温台的试样的装置。 本发明提供了一种新型结构的电子显微镜的相关低温试样的制备装置，其特征在于，为了通过将格栅插入液氮中连续地进行冷却网格的预处理，并且一系列安装过程 在荧光显微镜上通过转移预处理网格并在单个制备样品的装置内观察网格，通过解决现有电子显微镜的问题，其中在冷却的预处理中电网的损坏或破裂 频繁地产生电网和传送预处理电网并观察电网的过程，并且由于在传送预处理电网期间恒定地维持温度，电网的传输操作困难和不方便。

公开(公告)号：KR101388509B1

公开(公告)日：2014-04-28

申请号：KR1020130151602

申请日：2013-12-06

Applicant: 한국기초과학지원연구원

Inventor： 정현석 ,  현재경 ,  최의준 ,  전상미 ,  유동주 ,  송치홍

IPC: G01N1/42 ,  G01N33/48

Abstract: The present invention relates to a cryo plunger for cooling the sample grid to the temperature of liquid nitrogen which is to observe a biological sample using a transmission electron microscope (TEC) by a cryo analysis method, and according to the present invention, the present invention solves problems of conventional cryo sample plunger devices which are formed by a hydraulic device, the configuration is complicated, and manufacturing costs are high, and a spring type of a cryo sample plunger device which uses a spring structure thereby simplifying the configuration and correspondingly reducing manufacturing costs is provided.

Abstract translation: 本发明涉及一种用于将样品网格冷却至液氮温度的冷冻柱塞，该冷却柱塞使用透射电子显微镜（TEC）通过低温分析方法观察生物样品，根据本发明，本发明 解决了由液压装置形成的常规低温样品柱塞装置的问题，构造复杂，制造成本高，并且使用弹簧结构的冷冻样品柱塞装置的弹簧式，从而简化了构造并相应地减少制造 提供费用。