公开(公告)号：KR1020130124742A

公开(公告)日：2013-11-15

申请号：KR1020120048102

申请日：2012-05-07

Applicant: 한국산업기술대학교산학협력단

Inventor： 진송완 ,  이승열

IPC: G01N21/76 ,  G01N33/483

Abstract: The present invention relates to a method and an apparatus for measuring oxygen concentration, more particularly, to a method and an apparatus for measuring oxygen concentration which can measure the internal oxygen concentration of a microchamber without an oxygen sensor The present invention comprises: a step for irradiating light to a cell culture medium containing a first luminescent dye with the sensitivity to oxygen and a second luminescent dye with the insensitivity to the oxygen (a); a step for obtaining images on the light emitted from the first and second luminescent dyes depending on the irradiated light (b); and a step for measuring the oxygen concentration of the cell culture medium using information contained in the images on the brightness of the light emitted from the first and second luminescent dyes(c). According to the present invention, the oxygen concentration of the cell culture medium is measured without the oxygen sensor by using both the luminescent dye with the sensitivity to the oxygen and the luminescent dye with the insensitivity of the oxygen, thereby increasing the accuracy on the measurement of the internal oxygen concentration of an artificial scaffold. [Reference numerals] (AA) Start;(BB) End;(S10) Irradiating light to a cell culture medium containing a first luminescent dye and a second luminescent dye;(S20) Obtaining images on the light emitted from the first and second luminescent dyes;(S30) Measuring the oxygen concentration of the cell culture medium using obtained images

Abstract translation: 本发明涉及一种用于测量氧浓度的方法和装置，更具体地说，涉及一种测量氧气浓度的方法和装置，其可以测量没有氧传感器的微室的内部氧浓度本发明包括： 将光照射到对氧敏感的含有第一发光染料的细胞培养基和对氧不敏感的第二发光染料（a）; 根据照射的光（b）从第一和第二发光染料发出的光上获得图像的步骤; 以及使用包含在图像中的信息来测量从第一和第二发光染料（c）发射的光的亮度来测量细胞培养基的氧浓度的步骤。 根据本发明，通过使用具有氧敏感性的发光染料和氧不敏感的发光染料，通过使用氧传感器来测量细胞培养基的氧浓度，从而提高测量精度 的人造脚手架的内部氧浓度。 （AA）开始;（BB）结束;（S10）将光照射到含有第一发光染料和第二发光染料的细胞培养基中;（S20）从第一发光和第二发光染料发射的光上获得图像 染料;（S30）使用获得的图像测量细胞培养基的氧浓度