公开(公告)号：KR100813150B1

公开(公告)日：2008-03-17

申请号：KR1020060109550

申请日：2006-11-07

Applicant: 한국생명공학연구원

Inventor： 유장렬 ,  정화지 ,  민성란 ,  정원중 ,  김현태 ,  박주영 ,  허정혜

IPC: A01H1/00 ,  A01H1/06 ,  A01H4/00

CPC classification number: C12N15/8214 ,  C12N9/0004 ,  C12N15/8261 ,  Y02A40/146

Abstract: An MDH plastid transgenic plant is provided to show significantly increased growth rate, area of leaves, diameter of stem and bio-mass compared with a control plant, thereby being usefully used for increasing the photosynthesis amount or biomass of the plant. A transgenic C3 plant is characterized in that an MDH(malate dehydrogenase) gene derived from prokaryote such as Corynebacterium glutamicum, Rhodococcus, Oceanobacillus, Aspergillus, Methanothermus, Chaetomium, Methanopyrus, Bacillus, Methanocaldococcus, Magnaporthe, Phaeosphaeria, Gibberella, Desulfitobacterium, coccidioides, Thermus, Candidatus, Pyrococcus, Solibacter, Aurantimonas, Syntrophus, Enterococcus, Methanosphaera, Anopheles, Entamoeba, Yersinia, Mesorhizobium, Tribolium, Salmonella, Aurantimonas, Amycolatopsis, Escherichia coli, Apis, Burkhoderia, Bordetella, Pseudomonas and Aedes is inserted into plastid genome of the C3 plant. A method for preparing the transgenic plant comprises the steps of: (a) inserting the MDH gene sequence into a transforming vector to prepare an MDH plastid transforming vector; (b) introducing the MDH plastid transforming vector into a C3 plant or a culture cell of the C3 plant to prepare a transgenic body; (c) culturing the transgenic body; and (d) redifferentiating the cultured transgenic body through tissue culturing.

Abstract translation: 提供MDH质体转基因植物以显示与对照植物相比显着增加的生长速率，叶面积，茎直径和生物质量，从而有效地用于增加植物的光合作用量或生物量。 转基因C3植物的特征在于，从原核生物如谷氨酸棒杆菌，红球菌，海洋芽孢杆菌，曲霉，甲烷热原，毛壳菌，甲烷us us，芽孢杆菌，甲烷球菌，马鞭草，Ph ia，赤霉菌，脱硫杆菌，球虫，栖热菌中的MDH（苹果酸脱氢酶） ，Candidatus，Pyrococcus，Solibatus，Aurantimonas，Syntrophus，Enterococcus，Methanosphaera，按蚊，阿米巴，耶尔森氏菌，中生根瘤菌，Tribolium，沙门氏菌，Aurantimonas，Amycolatopsis，大肠杆菌，Apis，Burkhoderia，博德特氏菌，假单胞菌和Aedes被插入质体基因组 C3植物。 制备转基因植物的方法包括以下步骤：（a）将MDH基因序列插入转化载体中以制备MDH质体转化载体; （b）将MDH质体转化载体导入C3植物或C3植物的培养细胞以制备转基因体; （c）培养转基因体; 和（d）通过组织培养重新分化培养的转基因体。