公开(公告)号：KR101467629B1

公开(公告)日：2014-12-04

申请号：KR1020120051710

申请日：2012-05-15

Applicant: 한남대학교 산학협력단

Inventor： 최성호 ,  박현열 ,  이진아 ,  반병관

IPC: C07K14/435 ,  C12N5/071

Abstract: 본발명은인간세포배양용실크피브로인멤브레인의제조과정및 이제조과정으로제조된 실크피브로인멤브레인과이를포함하는인간세포배양용조성물등에관한것으로, 보다구체적으로는누에고치로부터추출한실크피브로인단백질에가교제를첨가하고방사선조사하여, 실크피브로인멤브레인을제조하는과정에관한것이다. 이방법으로제조된실크피브로인멤브레인은섬유결합이강하여세포배양중에해리될가능성이낮아다양한세포를효율적으로배양할수 있고, 표면에굴곡이있어세포가배양되기에적합하다. 따라서본 발명을이용하여인간세포가배양된실크피브로인멤브레인을제조하여외과환자의피부재생에사용할수 있을것으로기대된다.

公开(公告)号：KR1020130127879A

公开(公告)日：2013-11-25

申请号：KR1020120051710

申请日：2012-05-15

Applicant: 한남대학교 산학협력단

Inventor： 최성호 ,  박현열 ,  이진아 ,  반병관

IPC: C07K14/435 ,  C12N5/071

Abstract: The present invention relates to a process of preparing a silk fibroin membrane for culturing human cells, the silk fibroin membrane prepared by the process, and a composition containing the silk fibroin membrane for culturing human cells and, more particularly, to a process of preparing a silk fibroin membrane by adding a crosslinking agent to silk fibroin proteins extracted from cocoons and irradiating with ultrasonic waves. The silk fibroin membrane prepared by the process has strong fiber bond and low dissociation possibility during cell culture, thereby effectively culturing various cells due to the curve on the surface. Therefore, human cell-cultured silk fibroin membrane is prepared and is used for skin regeneration in a surgical patient.

Abstract translation: 本发明涉及一种制备用于培养人细胞的丝素蛋白膜的方法，通过该方法制备的丝素蛋白膜和含有用于培养人细胞的丝素蛋白膜的组合物，更具体地说，涉及制备 丝朊蛋白膜通过向蚕丝提取的丝素蛋白加入交联剂并用超声波照射。 通过该方法制备的丝素蛋白膜在细胞培养过程中具有较强的纤维结合和较低的解离可能性，从而由于表面上的曲线而有效地培养各种细胞。 因此，人体细胞培养的丝素蛋白膜被制备并用于手术患者的皮肤再生。