동결보존 이종 피부의 제조 방법 및 그로부터 제조된 동결보존 이종 피부
    2.
    发明公开
    동결보존 이종 피부의 제조 방법 및 그로부터 제조된 동결보존 이종 피부 有权
    用于制备保存的XENOGRAFT皮肤和CRYO保存的XENOGRAFT皮肤的方法

    公开(公告)号:KR1020130087773A

    公开(公告)日:2013-08-07

    申请号:KR1020120008899

    申请日:2012-01-30

    CPC classification number: A61L27/60 A61L27/362 A61L2430/34 C12N5/06

    Abstract: PURPOSE: A method for preparing a cryo-preserved xenograft skin and the cryo-preserved xenograft skin prepared thereby are provided to improve the survival rate of cells and to reduce manufacturing costs. CONSTITUTION: A method for preparing a cryo-preserved xenograft skin comprises the following steps of: extracting a hetero-skin; sterilizing the extracted hetero-skin with an acetyl hydroperoxide solution and an ethylene diamine tetraacetic acid; preparing a basic solution by mixing dimethyl sulfoxide, animal cell culture medium, and fetal bovine serum; preparing a cryoprotective agent by dissolving a sucrose in the basic solution; permeating the cryoprotective agent into the hetero-skin; and freezing the hetero-skin. [Reference numerals] (AA) Frozen preserved skin x 400; (BB) Glycerol-based preserved skin x 400

    Abstract translation: 目的:提供一种制备低温保存的异种移植皮肤的方法和由此制备的冷冻保存的异种移植物皮肤,以提高细胞的存活率并降低制造成本。 构成:制备低温保存的异种移植皮肤的方法包括以下步骤:提取异质皮肤; 用乙酰氢过氧化物溶液和乙二胺四乙酸对提取的异质皮肤进行灭菌; 通过混合二甲基亚砜,动物细胞培养基和胎牛血清制备碱性溶液; 通过将蔗糖溶解在碱性溶液中制备冷冻保护剂; 将冷冻保护剂渗透到异质皮肤中; 并冻结异体皮肤。 (附图标记)(AA)冷冻保存皮肤×400; (BB)基于甘油的保存皮肤×400

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