公开(公告)号：KR100385913B1

公开(公告)日：2003-06-02

申请号：KR1020000062095

申请日：2000-10-21

Applicant: 황재관

Inventor： 황재관 ,  최문정 ,  윤정미

IPC: C07H1/08

Abstract: PURPOSE: Provided is a method for manufacturing oligosaccharide from ginseng, in particular, from ginseng dregs generated from the manufacturing ginseng extract, by treating polysaccharide hydrolyase. CONSTITUTION: The manufacturing method comprises the steps of: heat-vaporizing or freeze-drying ginseng dregs to remove remaining moisture or solvent therefrom then followed by pulverizing; adding polysaccharide hydrolyase, such as cellulase, hemiplegia, and pectinase, to the ginseng dregs then hydrolyzing the ginseng dregs at 30-60 deg.C for 0.5-6 hours with stirring; heating the above enzyme treated sample at 100 deg.C for 10-30 minutes to inactivate the enzyme then centrifuging it at 5000-10000xg for 10-30 minutes to obtain oligosaccharide from the supernatant; and filtering the supernatant followed by passing it through ultrafiltrator with ultrafiltration membrane of MW 500-5000 then fractioning it according to molecular weight.

Abstract translation: 目的：提供一种由人参制备寡糖的方法，特别是由制造人参提取物产生的人参渣，通过处理多糖水解酶来制备。 本发明的制造方法包括以下步骤：将人参残渣热蒸发或冷冻干燥以除去残留的水分或溶剂，然后粉碎; 向人参糟中加入纤维素酶，偏瘫，果胶酶等多糖水解酶，然后在30-60℃下水解人参渣0.5-6小时， 将上述酶处理过的样品在100℃加热10-30分钟使酶失活，然后在5000-10000xg下离心10-30分钟，从上清液中获得寡糖; 过滤上清液，然后将其通过具有MW 500-5000的超滤膜的超滤器，然后根据分子量对其进行分级。

公开(公告)号：KR1020020031561A

公开(公告)日：2002-05-02

申请号：KR1020000062095

申请日：2000-10-21

Applicant: 황재관

Inventor： 황재관 ,  최문정 ,  윤정미

IPC: C07H1/08

Abstract: PURPOSE: Provided is a method for manufacturing oligosaccharide from ginseng, in particular, from ginseng dregs generated from the manufacturing ginseng extract, by treating polysaccharide hydrolyase. CONSTITUTION: The manufacturing method comprises the steps of: heat-vaporizing or freeze-drying ginseng dregs to remove remaining moisture or solvent therefrom then followed by pulverizing; adding polysaccharide hydrolyase, such as cellulase, hemiplegia, and pectinase, to the ginseng dregs then hydrolyzing the ginseng dregs at 30-60 deg.C for 0.5-6 hours with stirring; heating the above enzyme treated sample at 100 deg.C for 10-30 minutes to inactivate the enzyme then centrifuging it at 5000-10000xg for 10-30 minutes to obtain oligosaccharide from the supernatant; and filtering the supernatant followed by passing it through ultrafiltrator with ultrafiltration membrane of MW 500-5000 then fractioning it according to molecular weight.

Abstract translation: 目的：提供人参制造寡糖的方法，特别是由制造人参提取物产生的人参渣，通过处理多糖水解酶。 构成：制造方法包括以下步骤：将人参渣进行热蒸发或冷冻干燥以除去剩余的水分或溶剂，然后粉碎; 向人参渣中加入多糖水解酶，如纤维素酶，偏瘫和果胶酶，然后在30-60℃水解人参渣，搅拌0.5-6小时; 将上述酶处理的样品在100℃加热10-30分钟以使酶失活，然后以5000-10000xg离心10-30分钟以从上清液中获得寡糖; 并过滤上清液，然后将其通过超滤器与MW 500-5000的超滤膜，然后根据分子量进行分级。

公开(公告)号：KR1020020031266A

公开(公告)日：2002-05-01

申请号：KR1020000062434

申请日：2000-10-23

Applicant: 황재관

Inventor： 황재관 ,  이선희 ,  윤정미 ,  최문정

IPC: C12P19/26

Abstract: PURPOSE: Provided is a process of simply and economically manufacturing sialyloligosaccharides and peptides from chalaza of hen eggs by hydrolyzing chalaza proteins using proteases and fractionating them by molecular weight using ultrafiltration. CONSTITUTION: The manufacturing process comprises the steps of: washing chalaza with sodium chloride to remove remaining egg white therefrom; adding a protease to the chalaza to hydrolyze insoluble proteins; and fractionating protein hydrolysates using an ultrafiltrator by molecular weight to obtain sialyloligosaccharides and peptides. Wherein proteinases include Alcalase(Novo, Nordisk, Denmark), Neutrase(Novo, Nordisk, Denmark), Protamex(Novo, Nordisk, Denmark), Flavourzyme(Novo, Nordisk, Denmark), Collupulin(Gist-brocades Co., France) and Maxazyme(Gist-brocades Co., France).

Abstract translation: 目的：提供通过使用蛋白酶水解切拉沙蛋白并通过分子量使用超滤将它们分馏，从而简单且经济地制造唾液中的唾液酸寡糖和肽的方法。 规定：制造过程包括以下步骤：用氯化钠洗涤氯化钠以从其中除去剩余的蛋白; 向沙拉沙添加蛋白酶以水解不溶性蛋白质; 并使用超滤器通过分子量分级蛋白质水解产物以获得唾液酸寡糖和肽。 其中蛋白酶包括Alcalase（Novo，Nordisk，Denmark），Neutrase（Novo，Nordisk，Denmark），Protamex（Novo，Nordisk，Denmark），Flavourzyme（Novo，Nordisk，Denmark），Collupulin（Gist-brocades Co.，France） Maxazyme（Gist-brocades Co.，法国）。