公开(公告)号：US10047334B2

公开(公告)日：2018-08-14

申请号：US15064918

申请日：2016-03-09

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Sailaja Chandrapati ,  Heather M. Webb ,  Jeffrey C. Pederson ,  Jeffrey D. Smith ,  RuthAnn R. Duda ,  Brian J. Engel

IPC: B01L3/00 ,  C12M3/00 ,  C12M1/12 ,  C12Q1/22 ,  G01N21/64 ,  C12Q1/04 ,  C12M1/00

CPC classification number: C12M37/06 ,  C12M23/32 ,  C12M25/06 ,  C12Q1/04 ,  C12Q1/22 ,  G01N21/6486

Abstract: A biological sterilization indicator (BI). The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a substrate positioned in the housing between the first chamber and the second chamber. The substrate can be positioned in fluid communication with the first chamber and the second chamber, and the substrate can be further positioned such that the substrate is not in direct contact with the source of biological activity.

公开(公告)号：US20160186120A1

公开(公告)日：2016-06-30

申请号：US15064918

申请日：2016-03-09

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Sailaja Chandrapati ,  Heather M. Webb ,  Jeffrey C. Pederson ,  Jeffrey D. Smith ,  RuthAnn R. Duda ,  Brian J. Engel

IPC: C12M1/12 ,  C12M1/00

CPC classification number: C12M37/06 ,  C12M23/32 ,  C12M25/06 ,  C12Q1/04 ,  C12Q1/22 ,  G01N21/6486

Abstract: A biological sterilization indicator (BI). The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a substrate positioned in the housing between the first chamber and the second chamber. The substrate can be positioned in fluid communication with the first chamber and the second chamber, and the substrate can be further positioned such that the substrate is not in direct contact with the source of biological activity.

公开(公告)号：US09279141B2

公开(公告)日：2016-03-08

申请号：US14453797

申请日：2014-08-07

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Sailaja Chandrapati ,  Heather M. Webb ,  Jeffrey C. Pederson ,  Kurt J. Halverson ,  Brian J. Engel

IPC: C12Q1/22 ,  C12Q1/04 ,  G01N21/64

CPC classification number: C12M37/06 ,  C12M23/32 ,  C12M25/06 ,  C12Q1/04 ,  C12Q1/22 ,  G01N21/6486

Abstract: The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.

Abstract translation: 本发明提供了检测预定生物活性的方法。 该方法包括使用包含具有第一吸收光谱的第一指示剂和第二指示剂试剂的含水混合物。 第二指示剂通过预定的生物活性转化为具有第二发射光谱的第二生物衍生物。 第一吸收光谱包括在第二发射光谱中存在的波长的至少一部分中的可检测吸光度。 第一指示剂被含水液体接收并通过底物浓缩，便于检测第二生物衍生物。