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公开(公告)号：US09719125B2
公开(公告)日：2017-08-01
申请号：US15091024
申请日：2016-04-05
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Manjiri T. Kshirsagar , Kurt J. Halverson , Neil Percy , James E. Aysta
Abstract: The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.
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公开(公告)号：US09382570B2
公开(公告)日：2016-07-05
申请号：US14069416
申请日：2013-11-01
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Raj Rajagopal , Kurt J. Halverson , Manjiri T. Kshirsagar , James E. Aysta
IPC: C12M1/34 , C12M3/00 , C12M1/00 , C12Q1/68 , G01N15/06 , G01N33/00 , G01N33/48 , C12Q1/04 , B01L3/00 , C12M1/24 , C12M1/30 , G01N33/569 , A61B10/00 , A61B10/02
CPC classification number: C12Q1/04 , A61B10/0045 , A61B10/02 , B01L3/5082 , B01L2200/026 , B01L2200/10 , C12M23/08 , C12M23/32 , C12M23/34 , C12M33/02 , G01N33/56916 , G01N2333/245
Abstract: The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods.
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公开(公告)号：US20180051313A1
公开(公告)日：2018-02-22
申请号：US15556664
申请日：2016-03-15
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Raj Rajagopal , Manjiri T. Kshirsagar , Evan D. Brutinel , James E. Aysta
CPC classification number: C12Q1/24 , B01D46/00 , C12M1/12 , C12M1/26 , C12M1/34 , C12Q1/04 , G01N1/10 , G01N1/405 , G01N2001/4088
Abstract: Methods of detecting microorganisms in a fluid sample are provided. The method includes providing a nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, and contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article. The nonwoven article includes a fibrous porous matrix and a concentration agent particles enmeshed in the fibrous porous matrix. The method further includes placing the microorganism strain- or target cellular analyte-bound nonwoven article in contact with at least one detection reagent and detecting the presence of the bound microorganism strain or bound target cellular analyte. Devices and kits containing the devices, for contacting a fluid sample with a nonwoven article, are also provided. The device includes a sample container, a filter holder, a nonwoven article, and an adaptor configured to interface the filter holder with a receptacle.
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公开(公告)号：US10208330B2
公开(公告)日：2019-02-19
申请号：US15633983
申请日：2017-06-27
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Manjiri T. Kshirsagar , Kurt J. Halverson , Neil Percy , James E. Aysta
Abstract: The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.
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