公开(公告)号：WO1996040992A2

公开(公告)日：1996-12-19

申请号：PCT/US1996008070

申请日：1996-05-30

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  CARRINO, John, J. ,  BRAINARD, Thomas, D.

IPC: C12Q01/68

CPC classification number: C12Q1/686 ,  C12Q1/6848 ,  C12Q2537/107 ,  C12Q2525/186

Abstract: A method for reducing background caused by target-independent generation of amplification products, typically the products of a ligase chain reaction or a polymerase chain reaction, involves chemically "masking" or blocking the amplification probes or primers so that they cannot be extended or ligated until the occurence of a triggering event which can be delayed until the amplification reaction is begun. The probe masks take the form of complementary blocking oligonucleotides that are incapable of serving as template themselves and inhibit random tailing of the probe/primers. The blocking oligo masks are denatured from the probes during amplification and preferably are effectively eliminated from competing for probes in the amplification reaction

Abstract translation: 通过连接酶链反应或聚合酶链反应的产物，减少由靶标无关生成产生的背景引起的背景的方法涉及化学“掩蔽”或阻断扩增探针或引物，使得它们不能被延伸或连接直到 可以延迟直到扩增反应开始的触发事件的发生。 探针掩模采用不能用作模板本身并抑制探针/引物的随机拖尾的互补阻断寡核苷酸的形式。 在扩增过程中阻断寡核苷酸被探针变性，优选在扩增反应中与探针竞争中有效地消除

公开(公告)号：EP0832280A2

公开(公告)日：1998-04-01

申请号：EP96916811.0

申请日：1996-05-30

Applicant: Abbott Laboratories

Inventor： CARRINO, John, J. ,  BRAINARD, Thomas, D.

IPC: C12N15 ,  C12Q1

CPC classification number: C12Q1/686 ,  C12Q1/6848 ,  C12Q2537/107 ,  C12Q2525/186

Abstract: A method for reducing background caused by target-independent generation of amplification products, typically the products of a ligase chain reaction or a polymerase chain reaction, involves chemically 'masking' or blocking the amplification probes or primers so that they cannot be extended or ligated until the occurence of a triggering event which can be delayed until the amplification reaction is begun. The probe masks take the form of complementary blocking oligonucleotides that are incapable of serving as template themselves and inhibit random tailing of the probe/primers. The blocking oligo masks are denatured from the probes during amplification and preferably are effectively eliminated from competing for probes in the amplification reaction