公开(公告)号：WO1993010454A1

公开(公告)日：1993-05-27

申请号：PCT/US1992009362

申请日：1992-10-29

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  MERKH, Carl, W. ,  DEFREESE, James, D. ,  DURLEY, Bentley, A., III

IPC: G01N33/543

CPC classification number: B01L3/545 ,  G01N33/54366 ,  G01N33/6854 ,  G01N35/00029 ,  G01N2021/478 ,  G01N2035/00099 ,  G01N2035/00752 ,  G01N2035/1025 ,  G01N2201/0415 ,  G01N2201/0621 ,  G01N2201/1211

Abstract: A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immunoassays form human IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carousel is provided to position and hold a plurality of reaction cartridges (80). Each reaction cartridge (80) includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer contained within a reaction well (86) which is adapted to contain a biological sample to be assayed. The carousel and cartridges (80) contain structures which cooperate to precisely position the cartridges (80) in each of three separate dimensions so that each cartridge (80) is positioned uniformly. An optical reader operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge (80).

Abstract translation: 提供半自动生物样品分析仪和子系统以同时执行多种酶免疫测定，其形成对多个生物样品中的每一个中预先选择的过敏原组特异性的人IgE类抗体。 提供转盘以定位和保持多个反应盒（80）。 每个反应筒（80）包括在包含在反应井（86）内的固相结合层中的二维阵列中形成的多个分离的测试位点，其适于容纳要测定的生物样品。 圆盘传送带和墨盒（80）包含配合以将墨盒（80）精确地定位在三个分开的尺寸中的每一个中的结构，使得每个墨盒（80）均匀地定位。 提供以漫反射原理工作的光学阅读器，以从每个盒（80）的每个测试位点读取测定结果。