公开(公告)号：EP0381173A3

公开(公告)日：1991-01-09

申请号：EP90101859.8

申请日：1990-01-31

Applicant: ABBOTT LABORATORIES

Inventor： Georgevich, Gradimir G.

IPC: C12Q1/00 ,  C12Q1/42 ,  C12Q1/34 ,  C12Q1/28 ,  C12Q1/44 ,  G01N33/558

CPC classification number: G01N33/558

Abstract: An improved chromatographic device and method for quantitating the activity of an enzyme utilizes a bibulous test strip as the stationary phase, an aqueous solution as the mobile phase, and a chromophore as the indicator. The enzyme activity is quantitated by transforming a substantially depletable amount of a chromogenic substrate from soluble chromogen to insoluble chromophore as the mobile phase advances, whereupon the insoluble chromophore becomes immobilized on the test strip to produce a column of color. The length and/or the intensity of the column of color are related to the activity of the enzyme. The present invention is useful for the direct determination of enzyme-analytes and for the indirect determination of analytes which can be coupled to an enzyme.

公开(公告)号：EP0381173B1

公开(公告)日：1993-11-18

申请号：EP90101859.8

申请日：1990-01-31

Applicant: ABBOTT LABORATORIES

Inventor： Georgevich, Gradimir G.

IPC: C12Q1/00 ,  C12Q1/42 ,  C12Q1/34 ,  C12Q1/28 ,  C12Q1/44 ,  G01N33/558

CPC classification number: G01N33/558

公开(公告)号：EP0392377A2

公开(公告)日：1990-10-17

申请号：EP90106605.0

申请日：1990-04-06

Applicant: ABBOTT LABORATORIES

Inventor： Aunet, Diane L. ,  Elmore, Kristin D. ,  Georgevich, Gradimir G. ,  Oosta, Gary M. ,  Siegel, Neal A. ,  Jeng, Tzyy-Wen ,  Pry, Terry A. ,  Silverman, Cathy R.

IPC: G01N33/49 ,  B01D39/00

CPC classification number: B01L3/508 ,  B01D39/00 ,  B01D39/163 ,  B01D39/1661 ,  B01D39/18 ,  B01D39/201 ,  B01D39/2034 ,  B01D39/2075 ,  B01L2400/0406 ,  G01N33/491 ,  G01N33/526 ,  G01N33/5302

Abstract: A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum or plasma from the matrix.
According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix.
Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and eguivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis.
A further aspect of the present invention provides a porous reagent delivery system comprising a matrix of sintered porous material containing at least one reagent in a freeze-dried state, and method for making same. The system allows the delivery of a unitized form of a freeze-dried reagent which is recoverable upon contact with a liquid.

Abstract translation: 一种用于从全血中允许血浆或血清的分离装置和方法。 该装置包括用来将至少一个红血细胞凝集剂已经施加亲水性烧结的多孔材料的基质中。 。根据使用该装置的第一方法中，全血样品被施加到基质的第一端和进来与存在于基质中的凝集剂接触样品内的红血细胞。 血细胞凝集，并在基体的间隙被截留，同时基本上无血的细胞的血清或血浆累积装置的出口附近。 的滤波装置在与基质的功能芯吸从基质中的血清或血浆液体接收关系。 在本发明的备选方面。据，滤波器装置与矩阵函数的出口保留任何血细胞穿过基体作为过滤手段毛细作用从基质中的血浆或血清液体接收关系。 附加凝集剂可以掺入内的过滤器装置以促进血细胞穿过基体的保持。 本发明的另一个方面涉及一种用于将血清或血浆样品的测定中，利用烧结的多孔材料，颜色的矩阵：如烧结玻璃，烧结钢，烧结陶瓷，烧结塑料，和它们的eguivalents。 该矩阵是通过可再现的流体吸收能力正比于所述基质，血浆或血清成分的最小的反应性，以及亲水性内表面这使得矩阵收集和保留样本以进行分析的一个预定体积的固定尺寸为特征。 本发明的进一步的方面提供了一种多孔试剂递送系统包括包含在冷冻干燥状态的至少一种试剂用于制造相同的方法烧结的多孔材料，和的矩阵。 该系统允许一个冷冻干燥的试剂的所有其是在与液体接触的可采单元化形式的递送。

公开(公告)号：EP0392377B1

公开(公告)日：1995-02-15

申请号：EP90106605.0

申请日：1990-04-06

Applicant: ABBOTT LABORATORIES

Inventor： Aunet, Diane L. ,  Elmore, Kristin D. ,  Georgevich, Gradimir G. ,  Oosta, Gary M. ,  Siegel, Neal A. ,  Jeng, Tzyy-Wen ,  Pry, Terry A. ,  Silverman, Cathy R.

IPC: G01N33/49 ,  B01D39/00

CPC classification number: B01L3/508 ,  B01D39/00 ,  B01D39/163 ,  B01D39/1661 ,  B01D39/18 ,  B01D39/201 ,  B01D39/2034 ,  B01D39/2075 ,  B01L2400/0406 ,  G01N33/491 ,  G01N33/526 ,  G01N33/5302

公开(公告)号：EP0392377A3

公开(公告)日：1990-11-22

申请号：EP90106605.0

申请日：1990-04-06

Applicant: ABBOTT LABORATORIES

Inventor： Aunet, Diane L. ,  Elmore, Kristin D. ,  Georgevich, Gradimir G. ,  Oosta, Gary M. ,  Siegel, Neal A. ,  Jeng, Tzyy-Wen ,  Pry, Terry A. ,  Silverman, Cathy R.

IPC: G01N33/49 ,  B01D39/00

CPC classification number: B01L3/508 ,  B01D39/00 ,  B01D39/163 ,  B01D39/1661 ,  B01D39/18 ,  B01D39/201 ,  B01D39/2034 ,  B01D39/2075 ,  B01L2400/0406 ,  G01N33/491 ,  G01N33/526 ,  G01N33/5302

Abstract: A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum or plasma from the matrix. According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix. Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and eguivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis. A further aspect of the present invention provides a porous reagent delivery system comprising a matrix of sintered porous material containing at least one reagent in a freeze-dried state, and method for making same. The system allows the delivery of a unitized form of a freeze-dried reagent which is recoverable upon contact with a liquid.

公开(公告)号：EP0381173A2

公开(公告)日：1990-08-08

申请号：EP90101859.8

申请日：1990-01-31

Applicant: ABBOTT LABORATORIES

Inventor： Georgevich, Gradimir G.

IPC: C12Q1/00 ,  C12Q1/42 ,  C12Q1/34 ,  C12Q1/28 ,  C12Q1/44 ,  G01N33/558

CPC classification number: G01N33/558

Abstract: An improved chromatographic device and method for quantitating the activity of an enzyme utilizes a bibulous test strip as the stationary phase, an aqueous solution as the mobile phase, and a chromophore as the indicator. The enzyme activity is quantitated by transforming a substantially depletable amount of a chromogenic substrate from soluble chromogen to insoluble chromophore as the mobile phase advances, whereupon the insoluble chromophore becomes immobilized on the test strip to produce a column of color. The length and/or the intensity of the column of color are related to the activity of the enzyme. The present invention is useful for the direct determination of enzyme-analytes and for the indirect determination of analytes which can be coupled to an enzyme.

Abstract translation: 用于定量酶的活性的改进的色谱装置和方法利用吸水试纸作为固定相，作为流动相的水溶液和发色团作为指示剂。 通过随着流动相的进行将基本上可消耗量的显色底物从可溶性色原转化为不溶性发色团来定量酶活性，随后将不溶性发色团固定在测试条上以产生色谱柱。 色谱柱的长度和/或强度与酶的活性有关。 本发明可用于直接测定酶分析物和间接测定可与酶偶联的分析物。