公开(公告)号：WO1995025282A1

公开(公告)日：1995-09-21

申请号：PCT/US1995003168

申请日：1995-03-14

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HILTIBRAN, Robert, G. ,  JOU, Yi-Her ,  STROUPE, Stephen, D. ,  KLINE, Steven, J. ,  SCHULTZ, Steven, G.

IPC: G01N33/538

CPC classification number: G01N33/538

Abstract: This invention presents novel reagents, separation techniques and assay procedures which allow both the indicator and the capture reagents to be in solution to avoid problems of slowed reaction kinetics. The separation procedure involves a soluble capture reagent, comprising a specific binding member attached to a charged substance, and a insoluble solid phase that is oppositely charged with respect to the charged substance included in the capture reagent. A test sample suspected of containing the analyte of interest is mixed with the capture reagent to form a charged capture reagent/analyte complex. The reaction mixture is contacted to the oppositely charged solid phase to attract, attach, and separate the capture reagent/analyte complexes and any unreacted capture reagent from the reaction mixture. With an appropriate indicator reagent, i.e., a second specific binding member which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed.

Abstract translation: 本发明提出新型试剂，分离技术和测定方法，其允许指示剂和捕获试剂在溶液中以避免反应动力学减慢的问题。 分离方法包括可溶性捕获试剂，其包含与带电物质连接的特异性结合成员，以及相对于包含在捕获试剂中的带电物质相反带电的不溶性固相。 将怀疑含有感兴趣分析物的测试样品与捕获试剂混合以形成带电的捕获试剂/分析物复合物。 将反应混合物与相反电荷的固相接触以吸引，附着并将捕获试剂/分析物复合物和任何未反应的捕获试剂从反应混合物分离。 使用合适的指示剂，即与能够产生可检测信号的标签缀合的第二特异性结合成员，可以进行夹心和竞争性测定。