公开(公告)号：WO1993018067A1

公开(公告)日：1993-09-16

申请号：PCT/US1993002011

申请日：1993-03-04

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  KUNDU, Samar ,  LA FLEUR, Marie, Anne

IPC: C07K15/28

CPC classification number: C07K16/18 ,  G01N33/92 ,  G01N2800/044

Abstract: The present invention relates to a method for directly measuring concentrations of cholesterol associated with specific lipoproteins in a plasma sample. The method involves the specific capture of intact lipoprotein particles containing cholesterol from a plasma sample with a specific lipoprotein binding agent. The quantity of the specific lipoprotein cholesterol present in the sample is then measured by detecting the amount of binding-agent-lipoprotein complexes that have formed in the reaction. The cholesterol contained in the binding-agent-lipoprotein complexes can be detected by reacting the complexes with labeled cholesterol specific binding agents and measuring the amount of label bound thereto, or by releasing the cholesterol in the complexes and measuring the amount of cholesterol released. The specific lipoprotein binding agent can be bound to a solid support. The assay method may also incorporate a further step of separating the solid support from the sample before detecting the presence of cholesterol bound to the solid support.

Abstract translation: 本发明涉及直接测定血浆样品中与特定脂蛋白相关的胆固醇浓度的方法。 该方法包括用特异性脂蛋白结合剂特异性捕获含有来自血浆样品的胆固醇的完整脂蛋白颗粒。 然后通过检测在反应中形成的结合剂 - 脂蛋白复合物的量来测量样品中存在的特定脂蛋白胆固醇的量。 包含在结合剂 - 脂蛋白复合物中的胆固醇可以通过使复合物与标记的胆固醇特异性结合剂反应并测量与其结合的标记量或通过释放复合物中的胆固醇并测量释放的胆固醇的量来检测。 特异性脂蛋白结合剂可以结合固体支持物。 测定方法还可以包括在检测结合到固体支持物的胆固醇的存在之前从样品中分离固体支持物的另外的步骤。