公开(公告)号：WO1996009532A1

公开(公告)日：1996-03-28

申请号：PCT/US1995011922

申请日：1995-09-20

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  STIMPSON, Donald, I. ,  GORDON, Julian ,  HOIJER, Joanell, V.

IPC: G01N21/77

CPC classification number: G01N21/47 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/552 ,  G01N21/7703 ,  G01N33/54373 ,  C12Q2565/619 ,  C12Q2565/501 ,  C12Q2565/607

Abstract: A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

Abstract translation: 波导结合测定方法包括检测引导到波导中的光的散射，散射是在ev逝波的穿透深度内特异地结合波导的散射标记的结果。 波导可以是透明塑料或玻璃，并且通常通过寡核苷酸杂交或免疫捕获来进行结合。 光散射标签包括胶体金属或非金属，包括金，硒和胶乳。 也可以使用由染料或浓缩颗粒组成的光吸收构件来增强信号。 可以通过视觉或视频成像来监视实时的绑定和解离，例如使用CCD相机和帧抓取软件。 通过实时熔解曲线可以区分少量一个碱基的杂交错配。

公开(公告)号：WO1995014533A1

公开(公告)日：1995-06-01

申请号：PCT/US1994013539

申请日：1994-11-22

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  GORDON, Julian ,  STIMPSON, Donald, I. ,  HSIEH, Wang-Ting

IPC: B01L03/00

CPC classification number: G01N1/4077 ,  B01J3/006 ,  B01L3/502 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5635 ,  G01N33/53 ,  G01N2001/007 ,  G01N2001/4088 ,  Y10T436/25375 ,  Y10T436/255

Abstract: A method and apparatus for collecting a cell sample from a liquid specimen utilizes a collection receptacle (10) in which the specimen is deposited, with a filter media (20) being placed in communication with a discharge port (14) in the collection receptacle (10) and a transfer device (40) for drawing specimen from the collection receptacle (10) and through the filter (20) for capturing the cellular component of the specimen on the filter media (20) for defining a cell sample for analysis. The collection receptacle (10), filter media (20) and carrier therefor (18) and the transfer device (40) may be supplied in kit form for use in a clinical environment. A hypobaric vessel may be used and the transfer device (40) and this vessel (40) may also serve as a disposal receptacle for the liquid specimen passed through the filter media (20).

Abstract translation: 用于从液体样本收集细胞样品的方法和装置利用其中沉积样本的收集容器（10），过滤介质（20）被放置成与收集容器中的排出口（14）连通 10）和用于从收集容器（10）抽取样品并通过过滤器（20）的传送装置（40），用于将样品的细胞组分捕获在过滤介质（20）上，用于限定用于分析的细胞样品。 收集容器（10），过滤介质（20）及其载体（18）和转移装置（40）可以以套件形式供应，以用于临床环境。 可以使用低压容器，并且转移装置（40）和该容器（40）也可以用作通过过滤介质（20）的液体试样的处理容器。

公开(公告)号：WO1995011454A1

公开(公告)日：1995-04-27

申请号：PCT/US1994011015

申请日：1994-09-28

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  GORDON, Julian ,  KOTLARIK, John, J. ,  STIMPSON, Donald, I. ,  HOIJER, Joanell, V.

IPC: G01N33/50

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/50825 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0406 ,  B01L2400/0442 ,  C12Q1/68 ,  C12Q1/6837 ,  C12Q1/686 ,  G01N33/543 ,  G01N2001/282 ,  G01N2035/00752

Abstract: Methods, devices, apparatus and kits for amplifying and detecting ligands such as nucleic acids are provided. The apparatus may be a two-tier thermal cycling device that can operate in conjunction with a sealed reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction/detection unit. The reaction mixture may be transferred to the detection chamber by a heating element and expansion of a fluid. The target ligand becomes immobilized in a predetermined array on a support in the detection chamber. A video imaging system associated with the apparatus detects and analyzes the immobilized ligand. Images are captured, digitized and subjected to statistical analysis to improve the accuracy of the imaging method.

Abstract translation: 提供了用于扩增和检测配体如核酸的方法，装置，装置和试剂盒。 该装置可以是可与密封的反应/检测单元结合操作的双层热循环装置。 将样品装载到装置的反应室中，然后将其与检测室配合以形成反应/检测单元。 反应混合物可以通过加热元件转移到检测室并膨胀流体。 靶配体以预定的阵列固定在检测室中的载体上。 与该装置相关联的视频成像系统检测并分析固定的配体。 图像被捕获，数字化并进行统计分析以提高成像方法的准确性。

公开(公告)号：EP0724725B1

公开(公告)日：2003-04-02

申请号：EP94930509.8

申请日：1994-09-28

Applicant: Abbott Laboratories

Inventor： GORDON, Julian ,  KOTLARIK, John, J. ,  STIMPSON, Donald, I. ,  HOIJER, Joanell, V.

IPC: G01N33/50 ,  C12Q1/68

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/50825 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0406 ,  B01L2400/0442 ,  C12Q1/68 ,  C12Q1/6837 ,  C12Q1/686 ,  G01N33/543 ,  G01N2001/282 ,  G01N2035/00752

Abstract: Methods, devices, apparatus and kits for amplifying and detecting ligands such as nucleic acids are provided. The apparatus may be a two-tier thermal cycling device that can operate in conjunction with a sealed reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction/detection unit. The reaction mixture may be transferred to the detection chamber by a heating element and expansion of a fluid. The target ligand becomes immobilized in a predetermined array on a support in the detection chamber. A video imaging system associated with the apparatus detects and analyzes the immobilized ligand. Images are captured, digitized and subjected to statistical analysis to improve the accuracy of the imaging method.

公开(公告)号：EP0783683B1

公开(公告)日：2004-04-07

申请号：EP95933864.1

申请日：1995-09-20

Applicant: Abbott Laboratories

Inventor： STIMPSON, Donald, I. ,  GORDON, Julian ,  HOIJER, Joanell, V.

IPC: G01N21/77 ,  G01N33/543

CPC classification number: G01N21/47 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/552 ,  G01N21/7703 ,  G01N33/54373 ,  C12Q2565/619 ,  C12Q2565/501 ,  C12Q2565/607

公开(公告)号：EP0730495A1

公开(公告)日：1996-09-11

申请号：EP95902682.0

申请日：1994-11-22

Applicant: Abbott Laboratories

Inventor： GORDON, Julian ,  STIMPSON, Donald, I. ,  HSIEH, Wang-Ting

IPC: G01N33 ,  B01D29 ,  B01D35 ,  B01J3 ,  B01L3 ,  C12N15 ,  C12Q1 ,  G01N1

CPC classification number: G01N1/4077 ,  B01J3/006 ,  B01L3/502 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5635 ,  G01N33/53 ,  G01N2001/007 ,  G01N2001/4088 ,  Y10T436/25375 ,  Y10T436/255

Abstract: A method and apparatus for collecting a cell sample from a liquid specimen utilizes a collection receptacle (10) in which the specimen is deposited, with a filter media (20) being placed in communication with a discharge port (14) in the collection receptacle (10) and a transfer device (40) for drawing specimen from the collection receptacle (10) and through the filter (20) for capturing the cellular component of the specimen on the filter media (20) for defining a cell sample for analysis. The collection receptacle (10), filter media (20) and carrier therefor (18) and the transfer device (40) may be supplied in kit form for use in a clinical environment. A hypobaric vessel may be used and the transfer device (40) and this vessel (40) may also serve as a disposal receptacle for the liquid specimen passed through the filter media (20).

公开(公告)号：EP0783683A1

公开(公告)日：1997-07-16

申请号：EP95933864.0

申请日：1995-09-20

Applicant: Abbott Laboratories

Inventor： STIMPSON, Donald, I. ,  GORDON, Julian ,  HOIJER, Joanell, V.

IPC: C12Q1 ,  G01N21 ,  G01N33

CPC classification number: G01N21/47 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/552 ,  G01N21/7703 ,  G01N33/54373 ,  C12Q2565/619 ,  C12Q2565/501 ,  C12Q2565/607

Abstract: A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

公开(公告)号：EP0724725A1

公开(公告)日：1996-08-07

申请号：EP94930509.0

申请日：1994-09-28

Applicant: Abbott Laboratories

Inventor： GORDON, Julian ,  KOTLARIK, John, J. ,  STIMPSON, Donald, I. ,  HOIJER, Joanell, V.

IPC: B01L3 ,  B01L7 ,  C12Q1 ,  G01N21 ,  G01N33 ,  G06T1 ,  G01N1 ,  G01N35 ,  G01N37

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/50825 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0406 ,  B01L2400/0442 ,  C12Q1/68 ,  C12Q1/6837 ,  C12Q1/686 ,  G01N33/543 ,  G01N2001/282 ,  G01N2035/00752

Abstract: Methods, devices, apparatus and kits for amplifying and detecting ligands such as nucleic acids are provided. The apparatus may be a two-tier thermal cycling device that can operate in conjunction with a sealed reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction/detection unit. The reaction mixture may be transferred to the detection chamber by a heating element and expansion of a fluid. The target ligand becomes immobilized in a predetermined array on a support in the detection chamber. A video imaging system associated with the apparatus detects and analyzes the immobilized ligand. Images are captured, digitized and subjected to statistical analysis to improve the accuracy of the imaging method.