公开(公告)号：WO1996041200A1

公开(公告)日：1996-12-19

申请号：PCT/US1996006820

申请日：1996-05-13

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  TAKEDA, Masaaki ,  KATO, Yuko ,  PELLETIER, Brent, Allen

IPC: G01N35/10

CPC classification number: G01N35/1016 ,  G01M3/26 ,  G01N2035/102

Abstract: Embodiments described herein provide a pipetting apparatus with leak detection and a method for detecting a leak. In one embodiment, an automatic pipetting apparatus comprises a nozzle having a lower end portion through which a fluid sample is aspirated and dispensed by changes in air pressure supplied to the nozzle. A piping system operatively connects the nozzle to a pump for supplying the air pressure to the nozzle. A pressure sensor is operatively connected with the piping system for measuring internal pressure in the piping system. Means is operatively connected with the nozzle for three-dimensionally moving the nozzle between an aspirating position of the fluid sample and a dispensing position. Leak detecting means is operatively connected with the pressure sensor for detecting a leak in the piping system based upon a measured result obtained by measuring the internal pressure in the piping system with the pressure sensor while the nozzle, which has aspirated a predetermined volume of the fluid sample, is being moved between the aspirating position and the dispensing position by the moving means.

Abstract translation: 本文所述的实施例提供了具有泄漏检测的移液装置和用于检测泄漏的方法。 在一个实施例中，自动移液装置包括具有下端部分的喷嘴，通过该下端部抽吸流体样品并通过供给喷嘴的空气压力的变化来分配。 管道系统将喷嘴可操作地连接到泵，以将空气压力提供给喷嘴。 压力传感器与管道系统可操作地连接，用于测量管道系统中的内部压力。 装置与喷嘴可操作地连接，用于在流体样品的抽吸位置和分配位置之间三维地移动喷嘴。 泄漏检测装置与压力传感器可操作地连接，用于基于通过用压力传感器测量管道系统中的内部压力而获得的测量结果来检测管道系统中的泄漏，同时吸入预定体积的流体 样品通过移动装置在抽吸位置和分配位置之间移动。

公开(公告)号：EP0608425A1

公开(公告)日：1994-08-03

申请号：EP92921482.3

申请日：1992-10-15

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES

Inventor： KAWANABE, Junichi ,  TAKEDA, Masaaki ,  KATAGI, Hitomi ,  KATO, Yuko ,  BIELARCZYK, Gregory, A. ,  MAGEE, Rosie, L. c/o Ms. Annie Lewis

IPC: G01N35/06 ,  G01N11/08 ,  G01N11/06

CPC classification number: G01N35/109 ,  G01N11/08 ,  G01N33/49 ,  G01N2035/1025

Abstract: Viscosity of red corpuscles is measured while suction of the red corpuscles is performed in a dispensing device of blood specimen. In the process of dispensing red corpuscles as specimen, the control unit (84) draws the piston (76) at first to produce a specified initial suction pressure. The pressure sensor (54) monitors the internal pressure of the air tube (44). The viscosity measuring unit (88) measures the length of time from the application of initial suction pressure to achievement of a predetermined pressure value ß and, referring to the table (88) and on the basis of the measured length of time, find a viscosity of the red corpuscles. The table (88) stores the correlation between a length of time and a viscosity which are proportional to each other.

Abstract translation: 测量红血球的粘度，同时在血液样本的分配装置中进行红血球的抽吸。 在分配红色微粒作为试样的过程中，控制单元（84）首先拉动活塞（76）以产生特定的初始吸入压力。 压力传感器（54）监测空气管（44）的内部压力。 粘度测量单元（88）测量从施加初始吸入压力到达到预定压力值β的时间长度，并且参考表格（88）并且基于测量的时间长度发现粘度 的红色小体。 表格（88）存储彼此成比例的时间长度和粘度之间的相关性。

公开(公告)号：EP0658769B1

公开(公告)日：1999-10-13

申请号：EP93919597.0

申请日：1993-08-31

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES

Inventor： TAKEDA, Masaaki ,  KATO, Yuko ,  KATAGI, Hitomi

IPC: G01N35/10 ,  G01M3/26

CPC classification number: G01M3/26 ,  G01N35/1011 ,  G01N2035/1025

Abstract: A leakage detection method in an automatic pipetting apparatus for objectively judging leakage by measuring an internal pressure of a pump connected to a nozzle tip. At Step 202, distilled water (90) is sucked, and when an internal pressure P1 of a pump after completion of suction is near the atmospheric pressure, leakage is identified. At Step 203, the tip (36) is kept at halt for 30 seconds, for example, without allowing it to rise after the internal pressure P1 of the pump after completion of suction is measured at Step 202. The internal pressure P2 of the pump is again measured by a pressure sensor. When this internal pressure P2 is different from the internal pressure P1 previously measured, leakage is identified. Further, at Step 204, sucked distilled water (90) is discharged into the same test tube (62), and the internal pressure of the pump is monitored by the pressure sensor. If any sharp fluctuation of the internal pressure is found, leakage is judged. When leakage is detected, leakage detection measurement is terminated.

公开(公告)号：EP0830610A1

公开(公告)日：1998-03-25

申请号：EP96915759.0

申请日：1996-05-13

Applicant: Abbott Laboratories

Inventor： TAKEDA, Masaaki ,  KATO, Yuko ,  PELLETIER, Brent, Allen

IPC: G01M3 ,  G01N35

CPC classification number: G01N35/1016 ,  G01M3/26 ,  G01N2035/102

Abstract: Embodiments described herein provide a pipetting apparatus with leak detection and a method for detecting a leak. In one embodiment, an automatic pipetting apparatus comprises a nozzle having a lower end portion through which a fluid sample is aspirated and dispensed by changes in air pressure supplied to the nozzle. A piping system operatively connects the nozzle to a pump for supplying the air pressure to the nozzle. A pressure sensor is operatively connected with the piping system for measuring internal pressure in the piping system. Means is operatively connected with the nozzle for three-dimensionally moving the nozzle between an aspirating position of the fluid sample and a dispensing position. Leak detecting means is operatively connected with the pressure sensor for detecting a leak in the piping system based upon a measured result obtained by measuring the internal pressure in the piping system with the pressure sensor while the nozzle, which has aspirated a predetermined volume of the fluid sample, is being moved between the aspirating position and the dispensing position by the moving means.

Abstract translation: 本文所述的实施例提供了具有泄漏检测的移液装置和用于检测泄漏的方法。 在一个实施例中，自动移液装置包括具有下端部分的喷嘴，流体样品通过该下端部抽吸并通过供给喷嘴的空气压力的变化来分配。 管道系统将喷嘴可操作地连接到泵，以将空气压力提供给喷嘴。 压力传感器与管道系统可操作地连接，用于测量管道系统中的内部压力。 XYZ机器人与喷嘴可操作地连接，用于在流体样品的抽吸位置和分配位置之间三维地移动喷嘴。 泄漏检测器与压力传感器可操作地连接，用于基于通过用压力传感器测量管道系统中的内部压力而获得的测量结果来检测管道系统中的泄漏，同时吸入预定体积的流体 样品由机器人在抽吸位置和分配位置之间移动。

公开(公告)号：EP0658769A1

公开(公告)日：1995-06-21

申请号：EP93919597.0

申请日：1993-08-31

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES

Inventor： TAKEDA, Masaaki ,  KATO, Yuko ,  KATAGI, Hitomi

IPC: G01N35/06 ,  G01M3/26

CPC classification number: G01M3/26 ,  G01N35/1011 ,  G01N2035/1025

Abstract: A leakage detection method in an automatic pipetting apparatus for objectively judging leakage by measuring an internal pressure of a pump connected to a nozzle tip. At Step 202, distilled water (90) is sucked, and when an internal pressure P₁ of a pump after completion of suction is near the atmospheric pressure, leakage is identified. At Step 203, the tip (36) is kept at halt for 30 seconds, for example, without allowing it to rise after the internal pressure P₁ of the pump after completion of suction is measured at Step 202. The internal pressure P₂ of the pump is again measured by a pressure sensor. When this internal pressure P₂ is different from the internal pressure P₁ previously measured, leakage is identified. Further, at Step 204, sucked distilled water (90) is discharged into the same test tube (62), and the internal pressure of the pump is monitored by the pressure sensor. If any sharp fluctuation of the internal pressure is found, leakage is judged. When leakage is detected, leakage detection measurement is terminated.

Abstract translation: 一种自动移液装置中的泄漏检测方法，用于通过测量连接到喷嘴头的泵的内部压力来客观地判断泄漏。 在步骤202中，吸入蒸馏水（90），当抽吸完成后泵的内部压力P 1接近大气压时，识别泄漏。 在步骤203中，在步骤202中测量抽吸完成之后，在泵的内部压力P 1之后，尖端（36）例如保持停止30秒，而不使其升高。泵的内部压力P 2 再次由压力传感器测量。 当该内部压力P 2与先前测量的内部压力P 1不同时，识别泄漏。 而且，在步骤204中，将吸入的蒸馏水（90）排出到同一试管（62）中，利用压力传感器监视泵的内部压力。 如果发现内部压力急剧波动，则判断泄漏。 当检测到泄漏时，泄漏检测测量结束。

公开(公告)号：EP0726466B1

公开(公告)日：2002-04-24

申请号：EP93919596.2

申请日：1993-08-31

Applicant: Aloka Co. Ltd. ,  ABBOTT LABORATORIES

Inventor： TAKEDA, Masaaki ,  KATO, Yuko ,  KATAGI, Hitomi ,  KAWANABE, Junichi

IPC: G01N35/10

CPC classification number: G01N35/1016 ,  G01N35/10 ,  G01N2035/1018 ,  G06F19/00

Abstract: In a pipetting apparatus for pipetting a sample with a nozzle, imperfect nozzle clotting condition can be detected in addition to perfect nozzle clotting condition. ÄStructureÜ The pressure in the air hose 12 connected to the nozzle 10 for aspirating a sample 18 is detected by the pressure sensor 20, and the detected pressure value is transmitted to the clot detecting section 26. In this clot detecting section 26, a plurality of pressure calculation periods are set to calculate the pressure difference in each of the calculation periods. When at least one of the calculated pressure differences exceeds at least one of the predetermined threshold values corresponding to the calculation periods respectively, a clot alarm is generated. Since a plurality of the pressure calculation periods are set, an imperfect clotting condition can be detected in addition to the perfect clotting condition, thus improving pipetting accuracy.

公开(公告)号：EP0608423B1

公开(公告)日：1997-06-18

申请号：EP92921476.5

申请日：1992-10-15

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES

Inventor： KAWANABE, Junichi ,  TAKEDA, Masaaki ,  KATAGI, Hitomi ,  KATO, Yuko ,  BIELARCZYK, Gregory, A. ,  MAGEE, Rosie, L.

IPC: G01N35/10

CPC classification number: G01N35/109 ,  G01N2035/1025 ,  G01N2035/1034

Abstract: Object: to reduce the length of time for suction of red corpuscles as specimens of highly viscous liquid. Constitution: when suction of red corpuscles by the use of a nozzle tip is intended, suction is started with the piston retracted to the fullest extent so as to provide a maximum force of suction and, when pressure in the suction system reaches a fixed value α, the piston is restored to a point exhibiting a target amount of suction so that the target suction amount is finally attained. Suction speed can be expedited thanks to the suction force of the pump used to the largest allowable capability.

公开(公告)号：EP0726466A1

公开(公告)日：1996-08-14

申请号：EP93919596.2

申请日：1993-08-31

Applicant: Aloka Co. Ltd. ,  ABBOTT LABORATORIES

Inventor： TAKEDA, Masaaki ,  KATO, Yuko ,  KATAGI, Hitomi ,  KAWANABE, Junichi

IPC: G01N35/06

CPC classification number: G01N35/1016 ,  G01N35/10 ,  G01N2035/1018 ,  G06F19/00

Abstract: [Object]
In a pipetting apparatus for pipetting a sample with a nozzle, imperfect nozzle clotting condition can be detected in addition to perfect nozzle clotting condition.
[Structure]
The pressure in the air hose 12 connected to the nozzle 10 for aspirating a sample 18 is detected by the pressure sensor 20, and the detected pressure value is transmitted to the clot detecting section 26. In this clot detecting section 26, a plurality of pressure calculation periods are set to calculate the pressure difference in each of the calculation periods. When at least one of the calculated pressure differences exceeds at least one of the predetermined threshold values corresponding to the calculation periods respectively, a clot alarm is generated. Since a plurality of the pressure calculation periods are set, an imperfect clotting condition can be detected in addition to the perfect clotting condition, thus improving pipetting accuracy.

Abstract translation: 在用于用喷嘴移取样品的移液装置中，除了完美的喷嘴凝固条件之外，还可以检测到不完美的喷嘴凝固状况。 Ä结构通过压力传感器20检测连接到喷嘴10的用于抽吸样品18的空气软管12中的压力，并将检测到的压力值传输到凝块检测部分26.在该凝块检测部分26中， 设定压力计算周期，以计算每个计算周期中的压力差。 当计算出的压力差中的至少一个分别超过与计算周期对应的预定阈值中的至少一个时，产生凝块报警。 由于设定了多个压力计算期间，除了完美的凝固条件之外，还可以检测不完全的凝固条件，从而提高移液精度。

公开(公告)号：EP0608425B1

公开(公告)日：1997-06-18

申请号：EP92921482.3

申请日：1992-10-15

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES

Inventor： KAWANABE, Junichi ,  TAKEDA, Masaaki ,  KATAGI, Hitomi ,  KATO, Yuko ,  BIELARCZYK, Gregory, A. ,  MAGEE, Rosie, L. c/o Ms. Annie Lewis

IPC: G01N35/02

CPC classification number: G01N35/109 ,  G01N11/08 ,  G01N33/49 ,  G01N2035/1025

Abstract: Viscosity of red corpuscles is measured while suction of the red corpuscles is performed in a dispensing device of blood specimen. In the process of dispensing red corpuscles as specimen, the control unit (84) draws the piston (76) at first to produce a specified initial suction pressure. The pressure sensor (54) monitors the internal pressure of the air tube (44). The viscosity measuring unit (88) measures the length of time from the application of initial suction pressure to achievement of a predetermined pressure value β and, referring to the table (88) and on the basis of the measured length of time, find a viscosity of the red corpuscles. The table (88) stores the correlation between a length of time and a viscosity which are proportional to each other.

公开(公告)号：EP0608423A1

公开(公告)日：1994-08-03

申请号：EP92921476.5

申请日：1992-10-15

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES

Inventor： KAWANABE, Junichi ,  TAKEDA, Masaaki ,  KATAGI, Hitomi ,  KATO, Yuko ,  BIELARCZYK, Gregory, A. ,  MAGEE, Rosie, L.

IPC: G01N35/06

CPC classification number: G01N35/109 ,  G01N2035/1025 ,  G01N2035/1034

Abstract: Object: to reduce the length of time for suction of red corpuscles as specimens of highly viscous liquid. Constitution: when suction of red corpuscles by the use of a nozzle tip is intended, suction is started with the piston retracted to the fullest extent so as to provide a maximum force of suction and, when pressure in the suction system reaches a fixed value α, the piston is restored to a point exhibiting a target amount of suction so that the target suction amount is finally attained. Suction speed can be expedited thanks to the suction force of the pump used to the largest allowable capability.

Abstract translation: 对象：缩短吸入红色微粒作为高粘度液体样本的时间。 构成：当使用喷嘴头吸入红色微粒时，吸入开始于活塞最大程度地缩回，以提供最大的吸力，并且当吸气系统中的压力达到固定值α时 ，活塞恢复到表现出目标吸入量的点，从而最终达到目标吸入量。 由于所使用的泵的吸力最大，允许吸入速度加快。