公开(公告)号：WO1994016329A1

公开(公告)日：1994-07-21

申请号：PCT/US1994000429

申请日：1994-01-12

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  SWANSON, Jhan, C. ,  SONSALLA, Joy, C. ,  TZYY-WEN, Jeng

IPC: G01N33/53

CPC classification number: G01N33/54366 ,  G01N33/538 ,  G01N33/54313

Abstract: A method for the determination of the presence or amount of an analyte by monitoring a detectable label immobilized within an asymmetric membrane. A test mixture, comprising a test sample, an indicator reagent attached to a first specific binding pair member, and a capture reagent attached to a second specific binding pair member immobilized on a solid phase, is formed which is then captured within the asymmetric membrane thereby enabling analyte concentration to be determined.

Abstract translation: 通过监测固定在不对称膜内的可检测标记来确定分析物的存在或量的方法。 形成包含测试样品，连接到第一特异性结合对构件的指示剂和固定在固相上的第二特异性结合对构件附着的捕获试剂的测试混合物，然后将其捕获在非对称膜内 使分析物浓度得以确定。