公开(公告)号：EP1212623A1

公开(公告)日：2002-06-12

申请号：EP00964939.3

申请日：2000-09-05

Applicant: ABBOTT LABORATORIES

Inventor： LEE, Evelyn, Mok ,  WESTERBERG, David, A. ,  YAO, Haiou, H. ,  ADAMCZYK, Janina ,  CHRISTENSEN, Melissa, A.

IPC: G01N33/72 ,  G01N33/543

CPC classification number: G01N33/723 ,  Y10S435/96 ,  Y10S436/805 ,  Y10S436/824

Abstract: A glycated hemoglobin assay utilizes a simple procedure for the determination of DCCT Standardized %GHb in whole blood samples. First, a lysed whole blood sample is incubated with a solid phase that is coupled with boronic acid or similar boronate compound through covalent linkage chemistries known in the art. Next, a labeled antibody to human hemoglobin is added and the resulting signal is directly proportional to the %GHb in the sample. The advantages of measuring %GHb using a single determination include high precision and, since the assay is easily automatable, high throughput. With automation, this assay can also be consolidated with other testing on one analyzer. The method according to the various embodiments of the invention thus eliminates the need for two measurements: one for GHb and another for total hemoglobin (THb).

公开(公告)号：EP0974060A1

公开(公告)日：2000-01-26

申请号：EP98910389.0

申请日：1998-03-13

Applicant: Abbott Laboratories

Inventor： LEE, Evelyn, Mok ,  WESTERBERG, David, A. ,  YAO, Haiou, H. ,  ADAMCZYK, Janina ,  CHRISTENSEN, Melissa, A.

IPC: G01N33/72 ,  G01N33/543

CPC classification number: G01N33/723 ,  Y10S435/96 ,  Y10S436/805 ,  Y10S436/824

Abstract: A glycated hemoglobin assay utilizes a simple procedure for the determination of DCCT Standardized %GHb in whole blood samples. First, a lysed whole blood sample is incubated with a solid phase that is coupled with boronic acid or similar boronate compound through covalent linkage chemistries known in the art. Next, a labeled antibody to human hemoglobin is added and the resulting signal is directly proportional to the %GHb in the sample. The advantages of measuring %GHb using a single determination include high precision and, since the assay is easily automatable, high throughput. With automation, this assay can also be consolidated with other testing on one analyzer. The method according to the various embodiments of the invention thus eliminates the need for two measurements: one for GHb and another for total hemoglobin (THb).

公开(公告)号：EP0974060B1

公开(公告)日：2003-09-03

申请号：EP98910389.0

申请日：1998-03-13

Applicant: Abbott Laboratories

Inventor： LEE, Evelyn, Mok ,  WESTERBERG, David, A. ,  YAO, Haiou, H. ,  ADAMCZYK, Janina ,  CHRISTENSEN, Melissa, A.

IPC: G01N33/72 ,  G01N33/543

CPC classification number: G01N33/723 ,  Y10S435/96 ,  Y10S436/805 ,  Y10S436/824

Abstract: A glycated hemoglobin assay utilizes a simple procedure for the determination of DCCT Standardized %GHb in whole blood samples. First, a lysed whole blood sample is incubated with a solid phase that is coupled with boronic acid or similar boronate compound through covalent linkage chemistries known in the art. Next, a labeled antibody to human hemoglobin is added and the resulting signal is directly proportional to the %GHb in the sample. The advantages of measuring %GHb using a single determination include high precision and, since the assay is easily automatable, high throughput. With automation, this assay can also be consolidated with other testing on one analyzer. The method according to the various embodiments of the invention thus eliminates the need for two measurements: one for GHb and another for total hemoglobin (THb).