公开(公告)号：JP2001078765A

公开(公告)日：2001-03-27

申请号：JP24326499

申请日：1999-08-30

Applicant: ACADEMIA SINICA

Inventor： FUWAN JAURAN ,  SHAN HYUEI-FAN ,  CHEN TSUON-YUE

IPC: C12N15/09 ,  A61K39/104 ,  A61P31/04 ,  C07K14/21 ,  C12N1/21 ,  C12P21/02 ,  C12R1/38

Abstract: PROBLEM TO BE SOLVED: To obtain a fused polypeptide capable of inducing defensive immunity, and useful for vaccine preparation or the like by constituting the polypeptide from a receptor-bound domain or the like of an exotoxin A component of Pseudomanas. SOLUTION: This fused polypeptide comprises divisions represented by (i) a receptor-bound domain of an exotoxin A component of Pseudomonas, (ii) a membrane-metastasizing domain of the exotoxin A component of the Pseudomonas, (iii) a lipoprotein I of the Pseudomonas, or an antigenic fragment comprising at least 64 amino acid residues thereof, and (iv) an antigenic C- terminal fragment comprising at least 161 amino acid residues of the porin protein F of the Pseudomonas, and obtained by arranging the divisions from the N-terminus to the C-terminus in the order shown before. Preferably, the divisions (i) to (iv) are derived from Pseudomonas aeruginosa, and the fused polypeptide is produced by culturing a host cell transformed by a vector including a nucleic acid encoding the fused polypeptide. The vaccine preparation is preferably prepared by using the polypeptide.