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公开(公告)号：JP2011082181A
公开(公告)日：2011-04-21
申请号：JP2010255220
申请日：2010-11-15
Inventor： FISCHER STEVEN M , GOURLEY DARRELL L , BERTCH JAMES L
CPC classification number: H01J49/107 , H01J49/162 , H01J49/165 , H01J49/168
Abstract: PROBLEM TO BE SOLVED: To provide a multimode ionization source that can provide the advantages of combination of a plurality of ion sources, is not subjected to restrictions of individual ion sources, and does not require switching nor manual operation. SOLUTION: The multimode ionization source is provided with one or more atmospheric pressure ionization sources 3, 4. These ionization sources can perform electrospray ionization, atmospheric pressure chemical ionization and/or atmospheric pressure photoionization sources, and are used for ionizing molecules from a sample 21. A method of generating ions using the multimode ionization source is also disclosed. The apparatus and method provide the advantages of the combined ion sources, without the inherent disadvantages of the individual sources. COPYRIGHT: (C)2011,JPO&INPIT
Abstract translation: 要解决的问题：为了提供可以提供多个离子源的组合的优点的多模式电离源，不受单个离子源的限制，并且不需要开关或手动操作。 解决方案：多模电离源设置有一个或多个大气压离子源3,4，这些电离源可以执行电喷雾离子化，大气压化学电离和/或大气压光电离源，并用于从 样品21.还公开了使用多模式电离源产生离子的方法。 该装置和方法提供了组合离子源的优点，而没有各个源的固有缺点。 版权所有（C）2011，JPO＆INPIT
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公开(公告)号：JP2006120642A
公开(公告)日：2006-05-11
申请号：JP2005306589
申请日：2005-10-21
Inventor： FISCHER STEVEN M , GOURLEY DARRELL L , CORMIA PATRICIA H
CPC classification number: H01J49/107 , H01J49/06 , H01J49/165
Abstract: PROBLEM TO BE SOLVED: To provide an ion source capable of ensuring quality of a detected ESI signal by protecting ESI ions from APCI and APPI processes.
SOLUTION: A multimode ion source includes electrospray ion sources 8, 9 and 11 for providing a charged aerosol, atmospheric pressure ion sources 14 and 24 located in the downstream from the electrospray ion sources for further ionizing the charged aerosol, and a duct 20 adjacent to the atmospheric pressure ion sources, having an orifice for receiving ions from the charged aerosol and including a center shaft. The multimode ion source is provided with a mask 40 situated so as to separate a portion of the charged aerosol and prevent the portion from being exposed to the atmospheric pressure ionization source in the downstream.
COPYRIGHT: (C)2006,JPO&NCIPIAbstract translation: 要解决的问题：提供能够通过保护ESI离子从APCI和APPI过程来确保检测到的ESI信号的质量的离子源。 解决方案：多模式离子源包括电喷雾离子源8,9和11，用于提供位于电喷雾离子源下游的带电荷的气溶胶，大气压力离子源14和24，用于进一步电离带电气溶胶，以及管道 20，邻近大气压离子源，具有用于接收来自带电气溶胶的离子并包括中心轴的孔口。 多模式离子源设置有掩模40，掩模40被设置成分离带电气溶胶的一部分并防止该部分暴露于下游的大气压电离源。 版权所有（C）2006，JPO＆NCIPI
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公开(公告)号：JP2007010667A
公开(公告)日：2007-01-18
申请号：JP2006179042
申请日：2006-06-29
Inventor： PERKINS PATRICK D , FISCHER STEVEN M
CPC classification number: H01J49/165 , H01J49/107 , H01J49/145 , H01J49/162 , H01J49/168
Abstract: PROBLEM TO BE SOLVED: To provide a detection method for a complex specimen using a multimode ionization source capable of generating both an ESI ion and an APCI ion efficiently and effectively, using a single multimode ionization chamber and a single nebulizer.
SOLUTION: This detection method of the present invention includes (a) a step for introducing the complex specimen into an electrospray ionization source to generate charged aerosol, (b) a step for drying the charged aerosol by an infrared emitter adjacent to the electrospray ionization source, (c) a step for ionizing the dried charged aerosol, using an atmospheric pressure ionization source in a downstream of the electrospray ionization source, and (d) a step for detecting an ion from the complex specimen.
COPYRIGHT: (C)2007,JPO&INPITAbstract translation: 要解决的问题：使用单模多电离室和单一雾化器，能够有效且有效地使用能够同时产生ESI离子和APCI离子的多模电离源的复合样品的检测方法。 解决方案：本发明的检测方法包括（a）将复合试样引入电喷雾离子源产生带电气溶胶的步骤，（b）通过邻近 电喷雾离子源，（c）使用电喷射离子源的下游的大气压离子源离子化干燥的带电荷的气溶胶的步骤，以及（d）用于检测来自复合物的离子的步骤。 版权所有（C）2007，JPO＆INPIT
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4.发明专利
公开(公告)号：JP2004279424A
公开(公告)日：2004-10-07
申请号：JP2004069966
申请日：2004-03-12
Inventor： THOMPSON DEAN R , FISCHER STEVEN M
CPC classification number: G01N33/6848 , G01N30/72 , H01J49/0045 , Y10T436/143333 , Y10T436/24
Abstract: PROBLEM TO BE SOLVED: To provide a new method of identifying biopolymers and a program product used for the same. SOLUTION: The method of identifying a specific biopolymer in a sample comprises a step of selecting a mass from a first data set including measured masses of biopolymers and a step of matching masses in a second data set with the selected mass, wherein the second data set includes masses of a collection of fragments of the biopolymers, and wherein the matched masses represent fragments of the biopolymers with the selected mass, and comparing the matched masses to determine a monomer sequence for the biopolymer with the selected mass. COPYRIGHT: (C)2005,JPO&NCIPI
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5.发明专利Method of selecting n-terminal peptide and c-terminal peptide in proteomics 审中-公开选择N末端肽和C-末端肽在保守性中的方法
公开(公告)号：JP2004219418A
公开(公告)日：2004-08-05
申请号：JP2004004352
申请日：2004-01-09
Inventor： FISCHER STEVEN M
CPC classification number: G01N33/6803 , C07K1/36 , C12Q1/37 , G01N33/6848 , Y10S530/815 , Y10S530/816
Abstract: PROBLEM TO BE SOLVED: To provide an improved method capable of reducing the complexity of a sample, and capable of efficiently and highly reliably immobilizing and determining quantitatively a protein in the proteome sample. SOLUTION: The present invention provides the very efficient method for identifying the protein in the proteome sample by characterizing one N-terminal (or C-terminal) peptide per one protein, in one embodiment of the invention. The present invention provides the quantitative determination method related to a difference in an expression of the protein and/or modification thereof, as to the various kinds of samples, in another embodiment. The present invention provides a kit useful for executing conveniently the methods of the present invention, as an additional embodiment. COPYRIGHT: (C)2004,JPO&NCIPI
Abstract translation: 要解决的问题：提供一种能够降低样品复杂性并能够有效且高度可靠地固定和定量地确定蛋白质组学样品中的蛋白质的方法。 解决方案：在本发明的一个实施方案中，本发明提供了通过在每个蛋白质中表征一个N-末端（或C-末端）肽来鉴定蛋白质组样品中的蛋白质的非常有效的方法。 在另一个实施方案中，本发明提供了与各种样品相关的蛋白质和/或其修饰的表达差异的定量测定方法。 本发明提供了一种用于方便地执行本发明的方法的试剂盒，作为另外的实施方案。 版权所有（C）2004，JPO＆NCIPI
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Patent Agency Ranking
公开(公告)号：DE60320207T2
公开(公告)日：2009-05-14
申请号：DE60320207
申请日：2003-12-04
Applicant: AGILENT TECHNOLOGIES INC
Inventor： FISCHER STEVEN M
Abstract: Reducing (M1) the complexity of a proteomic sample, and preferably identifying proteins in the sample, comprises protecting the protein N- or C-termini with a suitable protecting agent, cleaving the protected proteins, separating the protected peptides from the peptide mixture, to reduce the sample complexity to one terminal peptide per sample protein, and, optionally, detecting the terminally protected peptides. Reducing (M1) the complexity of a proteomic sample, and preferably identifying proteins in the sample, comprises: (a) providing one or more proteins; (b) protecting the protein N- or C-termini with a suitable protecting agent; (c) cleaving the terminally protected proteins with a suitable cleaving agent to produce a mixture of terminally protected peptides and non-terminally protected peptides comprising free amino and carboxyl groups corresponding to the cleavage sites; (d) separating the terminally protected peptides from the peptide mixture, to reduce the sample complexity to one terminal peptide per sample protein; and, optionally (e) detecting the terminally protected peptides. Independent claims are also included for: (1) quantitatively comparing (M2) protein levels in two or more proteomic samples, comprising: (a) providing two or more samples each containing one or more proteins; (b) carrying out steps (b)-(d) of (M1), for each sample, thus reducing the sample complexity for each of the two or more protein samples to one terminal peptide per sample protein; (c) differentially labeling the terminally protected peptides of each sample with a suitable reagent comprising a detectable label, to generate two or more sets of differentially labeled terminal peptides; (d) measuring the relative levels of the differentially labeled terminal peptides, having optionally first combined the sets of labeled peptides; (2) a kit (II) for identifying proteins in a proteomic sample comprising one or more protecting agents for protecting the N- or C-termini, one or more cleaving agents, and means for separating the N- or C-terminally protected peptides from the mixture; and (3) a proteomic sample of reduced complexity obtainable by (M1).
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公开(公告)号：GB2441601A
公开(公告)日：2008-03-12
申请号：GB0706811
申请日：2007-04-05
Applicant: AGILENT TECHNOLOGIES INC
Inventor： FISCHER STEVEN M , CRAWFORD ROBERT K , FRAZER WILLIAM D
Abstract: The present Invention provides an ionization apparatus comprising an electrospray assembly 120 in an ionization chamber 110. Within the ionization chamber there are two electrodes 130, 135, each providing an associated electric field region. The first electrode 130 produces an electric field region which attracts the charged droplets from the electrospray tip 123 and the second electrode 135 produces an electric field region which repels the charged droplets. Furthermore there is a gas flow apparatus 124 provided which provides the charged droplets with sufficient velocity to overcome the repulsive forces of the second electric field region. This enables the charged droplets to pass through an interface 153 into a vacuum chamber 190 to be analyzed by apparatus such as a mass spectrometer 220.
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公开(公告)号：GB2443219B
公开(公告)日：2011-11-02
申请号：GB0702851
申请日：2007-02-14
Applicant: AGILENT TECHNOLOGIES INC
Inventor： RUSS CHARLES W , FISCHER STEVEN M , BERTSCH JAMES L
IPC: H01J49/04
Abstract: A method and apparatus for calibrating a mass spectrometry system in which lock mass ions are introduced into the transport region of a mass spectrometer intermittently in a pulsed manner and analyte ions and/or lock mass ions are then detected at the mass analyzer. In one embodiment, analyte ions are also introduced into the transport region of the mass spectrometer from the analyte ion source intermittently in a pulsed manner.
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公开(公告)号：DE602004012637T2
公开(公告)日：2009-04-16
申请号：DE602004012637
申请日：2004-02-12
Applicant: AGILENT TECHNOLOGIES INC
Inventor： THOMSON DEAN R , FISCHER STEVEN M
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公开(公告)号：DE60230761D1
公开(公告)日：2009-02-26
申请号：DE60230761
申请日：2002-05-02
Applicant: AGILENT TECHNOLOGIES INC
Inventor： FISCHER STEVEN M
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