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公开(公告)号：JPS54113800A
公开(公告)日：1979-09-05
申请号：JP2116578
申请日：1978-02-25
Applicant: ALOKA CO LTD
Inventor： NAKAZATO KAZUHISA , TOKUNAGA NOBORU , ARAYAMA HIROSHI
IPC: G21F9/06
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公开(公告)号：JPS6016598A
公开(公告)日：1985-01-28
申请号：JP12228183
申请日：1983-07-07
Applicant: ALOKA CO LTD
Inventor： TOKUNAGA NOBORU , ARAYAMA HIROSHI , AOYANAGI YOSHITO
IPC: C12Q1/06
Abstract: PURPOSE:To detect an amount of bacteria in air in a real time rapidly, by passing air to be tested through an extracting solution for a given time to extract ATP (adenosinemyrinic acid), adding a coloring reagent to it, detecting luminous energy of emission electrically. CONSTITUTION:The extracting solution 100 consisting of tris EDTA buffer solution is sent to the extraction device 10 consisting of the bubbling tube 12 and the collecting well 14, it is made into a boiling state, air in the chamber 102 is sucked by the pump 24, the sucked air is brought into contact with the extracting solution sufficiently, and ATP is extracted from bacteria contained in air. Impurities are removed from the solution having extracted ATP by the filter 32, cooled by the cooler 34, sent to the detection cell 30. A given amount of a coloring reagent (e.g., luciferin, or luciferase) 104 is added to it, emission of an amount of ATP, namely emission proportional to an amount of bacteria is caused, the emission is measured electrically by the photoelectron amplifying tube 40, and the amount of bacteria is outputted as a digital value by the calculator 42 for luminous energy.
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公开(公告)号：JPS5771400A
公开(公告)日：1982-05-04
申请号：JP14635580
申请日：1980-10-21
Applicant: Aloka Co Ltd
Inventor： ARAYAMA HIROSHI , TOKUNAGA NOBORU , TAGUCHI IKUO
Abstract: PURPOSE: To analyze minor constituents in a specimen in a short time with a high accuracy, by adding a luminous reagent capable of emitting light on reaction with a peroxidase to the specimen to emit the light.
CONSTITUTION: A luminous (light emitting) reagent consisting of luminol and hydrogen peroxide is added to a specimen containing a peroxidase to emit light, and the peroxidase is determined by the quantity of the emitted light. A vial 14 containing a sample prepared by adding the luminous (light emitting) reagent to the specimen is accommodated in a dark box 10, and a shutter 18 is closed to open a shutter 26. The light emitted in the vial 14 passes through a transparent plate 20 and enters a photomultiplier tube 24, and the resultant electric signals are counted by an amplifying and counting circuit 28 for a time set by a timer 32 and indicated in a display part 30.
COPYRIGHT: (C)1982,JPO&JapioAbstract translation: 目的：以高精度在短时间内分析标本中的次要成分，通过添加能够与过氧化物酶反应而发光的发光试剂发光。 构成：将含有鲁米诺和过氧化氢的发光（发光）试剂加入含有过氧化物酶的样品发光，过氧化物酶由发射光量决定。 包含通过将发光（发光）试剂添加到样品而制备的样品的小瓶14容纳在暗箱10中，并且快门18关闭以打开快门26.发射在小瓶14中的光穿过透明 板20并进入光电倍增管24，并且由放大和计数电路28对由此产生的电信号进行定时计时并由显示部分30表示的时间进行计数。
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公开(公告)号：JPS5771399A
公开(公告)日：1982-05-04
申请号：JP14635480
申请日：1980-10-21
Applicant: Aloka Co Ltd
Inventor： ARAYAMA HIROSHI , TOKUNAGA NOBORU , TAGUCHI IKUO
Abstract: PURPOSE: To analyze minor constituents in a specimen in a short time with a high accuracy, by measuring the quantity of light emitted by adding a luminous light emitting reagent to hydrogen peroxide generated due to the action of an enzyme on the specimen.
CONSTITUTION: The quantitative analysis is carried out by sensing the light emitted in the presence of luminol and a peroxidase from hydrgen peroxide generated by the action of an oxidase on a specimen, e.g. in the determination of glucose by a glucose oxidase, with a photomultiplier tube 24, etc. A sample prepared by adding the luminol and peroxidase to the specimen containing hydrogen peroxide is injected into a vial 14 and contained in an accommodating part 12 in a dark box 10, and a shutter 18 is closed. A shutter 26 is then opened, and light passes through a transparnt plate 20 and enters the photomultiplier tube 24. The light is then counted as electric signals by an amplifying and counting circuit 28 and then indicated in a display part 30.
COPYRIGHT: (C)1982,JPO&JapioAbstract translation: 目的：为了在短时间内高精度地分析样品中的次要成分，可以通过将由发光试剂产生的过氧化氢所产生的过氧化氢加入发光试剂中而发出的光量。 构成：定量分析是通过检测鲁米诺存在下发出的光和通过氧化酶作用在样品上产生的过氧化氢酶的过氧化物酶进行的。 通过葡萄糖氧化酶，光电倍增管24等测定葡萄糖。通过将含有过氧化氢的标本加入鲁米诺和过氧化物酶制备的样品注入小瓶14中，并将其包含在暗盒中的容纳部分12中 10，并且快门18关闭。 然后打开快门26，并且光通过透明板20并进入光电倍增管24.然后，光被放大和计数电路28计数为电信号，然后在显示部分30中指示。
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公开(公告)号：JPS5616873A
公开(公告)日：1981-02-18
申请号：JP9214079
申请日：1979-07-21
Applicant: ALOKA CO LTD
Inventor： TOKUNAGA NOBORU , KOBAYASHI KASHIWA , MINAGAWA EIICHI
IPC: G01N33/497 , G01N33/50
Abstract: PURPOSE:To make it possible to measure simply and accurately pollution components, specially, such as a radiation exposure dose in expiration, by providing a fixed-quantity expiration leading-in unit, a vessel where pollution components in expiration and reaction liquid react, and so on. CONSTITUTION:The gathering unit for pollution components in expiration is constituted by sample vessel 3 which gathers pollution components in expiration, reaction liquid supply pump 48 which supplies to sample vessel 38 a fixed quantity of reaction liquid which reacts on pollution components to absorb them, expiration leading-in tube 54 which blows expiration into reaction liquid in vessel 38, expiration flow meter 62 which measures the expiration quantity blown into reaction liquid, a control unit which stops blowing expiration into reaction liquid when the expiration quantity reaches a prescribed value, and so on. Then, in case of pollution components in expiration are tritium water, water or mixture liquid of water and ethylene glycol is used as reaction liquid and is reacted in vessel 38, and scintillator liquid 80 is led into vessel 38, and a radiation dose is measured by a scintillation counter and so on.
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Patent Agency Ranking
公开(公告)号：JPS52106782A
公开(公告)日：1977-09-07
申请号：JP1196376
申请日：1976-02-06
Applicant: ALOKA CO LTD
Inventor： KOTANINO AKIRA , TOKUNAGA NOBORU , TAKEDA MASAAKI
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公开(公告)号：JPH06182234A
公开(公告)日：1994-07-05
申请号：JP34270792
申请日：1992-12-22
Applicant: ALOKA CO LTD
Inventor： TOKUNAGA NOBORU , TAKEDA MASAAKI
Abstract: PURPOSE:To provide the disposable tip for dispensing blood into another container with good accuracy in the state of hermetically closing a vacuum blood collecting tube in blood inspection. CONSTITUTION:This disposable tip 16 has >=1 vent paths 18 provided integrally with its side wall. These vent paths 18 have upper vent ports 19a provided at the top end of the disposable tip 16 fitting to a nozzle base and lower vent ports 19b provided in any part from the top end of the disposable tip 16 to a sample and suction port. The outdoor air is infiltrated into the vacuum blood collecting tube 28 through the vent paths 18 until the same pressure as the atm. pressure is attained simultaneously when the disposable tip 16 is inserted into the vacuum blood collecting tube 28 by provision of these vent paths 18. As a result, the sample is dispensed with the good accuracy by using a pump.
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公开(公告)号：JPS6016586A
公开(公告)日：1985-01-28
申请号：JP12228383
申请日：1983-07-07
Applicant: ALOKA CO LTD
Inventor： TOKUNAGA NOBORU , ARAYAMA HIROSHI , AOYANAGI YOSHITO
Abstract: PURPOSE:To measure an amount of bacteria in a short time in a stabilized accuracy, by extracting ATP (adenosinetriphosphoric acid) contained in bacteria, adding a coloring agent to it, detecting luminous energy of emission electrically. CONSTITUTION:The bacteria-containing sample (e.g., filter sample having adsorbed bacteria) 20 is immersed in the extracted solution 102 such as boiling solution of tris (20mM) EDTA (2mM) at 7.75pH in the extraction cell 24. After a given time is passed, an extracted solution having extracted ATP is sent through the filter 40 to the detection cell 38, the coloring reagent (e.g., luciferin, or luciferase) 104 is added separated to it, emission in the detection cell 38 is outputted as a signal proportional to an amount of emission by the detector 48 for luminous energy consisting of a photoelectron amplifying tube, etc. set neighbor on the bottom of the cell 38, and fed to the display 50 for luminous energy and the display 52 for number of bacteria to show given display.
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公开(公告)号：JPS6016585A
公开(公告)日：1985-01-28
申请号：JP12228283
申请日：1983-07-07
Applicant: ALOKA CO LTD
Inventor： TOKUNAGA NOBORU , ARAYAMA HIROSHI , AOYANAGI YOSHITO
Abstract: PURPOSE:A bacteria monitor capable of measuring an amount of bacteria contained in air in high accuracy continuously, wherein a roll filter for bacteria absorption is used, it is exposed to an air path to introduce air in a room for a given time, and it is wound. CONSTITUTION:The roll filter 12 suspended between the reeling roll 14 and the winding roll 16 is set in such a way that it crosses the air path 10 by the switch flange part 10a, and closes it. The flange part 10a is closed to attach the roll filter 12 by pressure and to fix it, the air flow 100 in a room is made to flow for a given time, and bacteria in air are adsorbed and collected by the filter 12. The flange part 10 is opened, the adsorbing part of the filter 12 is sent to the punching part 18, the sample 20 having absorbed bacteria is punched, sent to the bacteria counter 22, to measure an amount of bacteria.
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公开(公告)号：JPS55155234A
公开(公告)日：1980-12-03
申请号：JP6222979
申请日：1979-05-22
Applicant: ALOKA CO LTD
Inventor： ARAYAMA HIROSHI , TOKUNAGA NOBORU , TAGUCHI IKUO
IPC: G01N33/483 , C12M1/34 , G01N21/76
Abstract: PURPOSE:To achieve quick analysis of trace component in the test piece with a better sensitivity by shaking the mixture of the test piece and the reagent which causes luminance when added thereto in the black box and the electric output of the photomultiplier tube is counted. CONSTITUTION:The testpiece and the reagent which causes luminance when added thereto are put in and mixed in the vial 14 outside the black box and the switch is turned on simultaneously with the mixing. With the mixing, the test piece and the reagent starts reaction and a luminance is caused corresponding to the trace component in the test piece. The vial 14 is inserted into the storage 12 and the test piece and the reagent are mixed quickly by shaking with the shaker 42. Then, the first optical shutter 18 is closed while the second shutter is opened. The luminance in the vial 14 enters the light receiving surface 24a of the photoelectric multiplier 24, which provides the electric output proportional to the level of the luminance. The output is counted by the amplification counting circuit 28, where the counting time is controlled as preset by the timer of the time setting circuit 32. The counted value of the circuit 28 is indicated on the display 30 for analysis and indication of trace components.
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