公开(公告)号：WO1984003152A1

公开(公告)日：1984-08-16

申请号：PCT/US1984000151

申请日：1984-02-03

Applicant: AMGEN

Inventor： AMGEN ,  EGRIE, Joan

IPC: G01N33/54

CPC classification number: C07K16/22 ,  G01N33/746 ,  Y10S435/803 ,  Y10S435/948 ,  Y10S436/817 ,  Y10S436/824 ,  Y10S530/808 ,  Y10S530/809

Abstract: A new mouse-mouse hybridoma tumor cell line A.T.C.C. No HB8209. A monoclonal antibody produced by said cell line is specifically immunologically reactive with erythropoietin and with a polypeptide whose amino acid sequence is substantially duplicative of a sequence extant in erythropoietin. Disclosed also are procedures for isolation of erythropoietin by affinity purification and for quantitative detection of erythropoietin in fluid samples.