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    IMAGING PLATFORM FOR NANOPARTICLE DETECTION APPLIED TO SPR BIOMOLECULAR INTERACTION ANALYSIS
    1.
    发明申请
    IMAGING PLATFORM FOR NANOPARTICLE DETECTION APPLIED TO SPR BIOMOLECULAR INTERACTION ANALYSIS 审中-公开
    用于SPR生物分子相互作用分析的纳米尺度检测的成像平台

    公开(公告)号：WO2004102160A3

    公开(公告)日：2005-11-10

    申请号：PCT/US2004006006

    申请日：2004-02-27

    Applicant: AMNIS CORP ,  JORGENSON RALPH ,  BASIJI DAVID ,  ORTYN WILLIAM

    Inventor： JORGENSON RALPH ,  BASIJI DAVID ,  ORTYN WILLIAM

    IPC: G01N21/25 ,  G01N21/65 ,  G01N21/75 ,  G01N33/543 ,  G01N21/55

    CPC classification number: G01N33/54346 ,  G01N21/25 ,  G01N21/554 ,  G01N21/648 ,  G01N21/658 ,  G01N21/75 ,  G01N33/54373

    Abstract: A flow imaging system is used to implement surface plasmon resonance (SPR) detection to study bio-molecular interactions. The flow imaging system is used to capture SPR absorption spectra of large numbers of objects, where each object includes both a metal film capable of exhibiting SPR, and detecting molecules. Analyte molecules are added to a solution of such objects, and the result is introduced into the flow imaging system which collects full SPR spectral data from individual objects. The objects can be nanoparticles or larger particles that support metal island films. The SPR spectral data can be used to determine specificity, kinetics, affinity, and concentration with respect to the interactions between the detecting molecules and the analyte molecules.

    Abstract translation: 流动成像系统用于实现表面等离子体共振（SPR）检测，以研究生物分子相互作用。 流动成像系统用于捕获大量物体的SPR吸收光谱，其中每个物体都包含能够显示SPR的金属膜和检测分子。 将分析物分子加入到这些物体的溶液中，并将其结果引入到从各个物体收集完整的SPR光谱数据的流动成像系统中。 物体可以是支撑金属岛膜的纳米颗粒或更大的颗粒。 SPR光谱数据可用于确定相对于检测分子和分析物分子之间的相互作用的特异性，动力学，亲和力和浓度。

    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS
    3.
    发明申请
    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS 审中-公开
    用于高速物体分析的现场成像的扩展深度

    公开(公告)号：WO2007067999A3

    公开(公告)日：2007-11-29

    申请号：PCT/US2006061871

    申请日：2006-12-11

    Applicant: AMNIS CORP ,  ORTYN WILLIAM ,  BASIJI DAVID ,  FROST KEITH ,  LIANG LUCHUAN ,  BAUER RICHARD ,  HALL BRIAN ,  PERRY DAVID

    Inventor： ORTYN WILLIAM ,  BASIJI DAVID ,  FROST KEITH ,  LIANG LUCHUAN ,  BAUER RICHARD ,  HALL BRIAN ,  PERRY DAVID

    IPC: G02F1/01

    CPC classification number: G02B27/0075 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1452 ,  G02B21/365 ,  G02B27/0012

    Abstract: A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.

    Abstract translation: 改进了高速，高分辨率流动成像系统，以实现扩展的景深成像。 光学失真元件被引入到流动成像系统中。 来自诸如单元的物体的光被失真元件扭曲，使得成像系统的点扩散函数（PSF）在扩展的景深范围内是不变的。 失真光被光谱分散，并且分散的光被用于同时产生多个图像。 检测图像，并且使用图像处理来通过补偿失真来增强检测到的图像，以实现对象的扩展景深图像。 后图像处理优选地涉及去卷积，并且需要由光学失真元件修改的成像系统的PSF的知识。

    Tdi imaging system for kinetic studies
    5.
    发明专利
    Tdi imaging system for kinetic studies 未知

    公开(公告)号：AU8838501A

    公开(公告)日：2002-03-04

    申请号：AU8838501

    申请日：2001-08-24

    Applicant: AMNIS CORP

    Inventor： BASIJI DAVID ,  ORTYN WILLIAM

    IPC: G01J3/28 ,  G01N15/14 ,  G01N21/47 ,  G01J3/14 ,  G01J3/443 ,  G01N21/64

    Abstract: Light from an object such as a cell moving through an imaging system is collected, and imaged onto a time delay integration (TDI) detector, producing a pixelated output signal in response to the image of the object. The light can be emitted from a luminous object, from a source and scattered by the object, or can be a fluorescent emission by one or more object probes. Light absorbed or reflected by the object can also produce images for determining specific characteristics of the object. In one set of embodiments, the movement of the object is synchronized with that of the pixelated output signal, which is controlled by the readout rate of the TDI detector. Alternatively, the readout rate of the pixelated output signal is not synchronized with the movement of the object, thereby permitting multiple signals to be produced for each of a plurality of objects over time.

    METHODS FOR ANALYZING INTER-CELLULAR PHENOMENA
    6.
    发明申请
    METHODS FOR ANALYZING INTER-CELLULAR PHENOMENA 审中-公开
    方法分析细胞内细胞

    公开(公告)号：WO2006118857A3

    公开(公告)日：2007-02-22

    申请号：PCT/US2006015491

    申请日：2006-04-20

    Applicant: AMNIS CORP ,  ORTYN WILLIAM ,  BASIJI DAVID ,  LYNCH DAVID

    Inventor： ORTYN WILLIAM ,  BASIJI DAVID ,  LYNCH DAVID

    IPC: G01N33/53

    CPC classification number: G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

    Abstract: Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least one characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological cells. In a particularly preferred, but not limiting implementation, the plurality of images for each individual object are collected simultaneously. In an empirical study, the characteristic being measured involves the synapse between conjugated cells. The conjugated cells may represent a subpopulation of the overall population of objects that were imaged. In a particularly preferred, yet not limiting embodiment, the present invention enables the quantization of the redistribution of cellular molecules due to the conjugation of different biological cells. Significantly, such quantization is not feasible with standard microscopy and flow cytometry.

    Abstract translation: 本发明的方面包括从对象群体收集多光谱图像，以及使用在图像集合中可识别的光度和/或形态测量特征来分析收集的图像以测量群体的至少一个特征。 在示例性应用中，对象是生物细胞。 在特别优选但不是限制的实现中，用于每个单独对象的多个图像被同时收集。 在实证研究中，所测量的特征涉及共轭细胞之间的突触。 共轭细胞可以代表成像对象的总体群体的亚群。 在特别优选的但不是限制性的实施方案中，本发明使得由于不同生物细胞的共轭而能够量化细胞分子的再分布。 值得注意的是，这种量化在标准显微镜和流式细胞术中是不可行的。

    METHOD AND APPARATUS FOR LABELING AND ANALYZING CELLULAR COMPONENTS
    7.
    发明申请
    METHOD AND APPARATUS FOR LABELING AND ANALYZING CELLULAR COMPONENTS 审中-公开
    用于标记和分析细胞成分的方法和设备

    公开(公告)号：WO02100157A3

    公开(公告)日：2003-04-10

    申请号：PCT/US0205557

    申请日：2002-02-21

    Applicant: AMNIS CORP

    Inventor： ORTYN WILLIAM ,  BASIJI DAVID

    IPC: C12Q1/68 ,  G01N27/447 ,  C07H21/04

    CPC classification number: G01N27/44726 ,  C12Q1/6841 ,  G01J3/2803 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6456 ,  G01N2015/1472 ,  G01N2021/6421 ,  G01N2021/6441 ,  C12Q2565/102 ,  C12Q2537/143

    Abstract: A labeling method that labels an object or specific features of an object with labeled probes that provide a multiplexed signal that can be analyzed by spectral decomposition. This binary and higher encoding scheme can be employed to label components of biological cells. In each encoding scheme, labeled probes that selectively bind to a specific feature are required. The labeled probes include a binding element that binds to the feature, and at least one signaling component that generates a detectable signal, preferably a spectral signature. In one embodiment, adding multiple fluorescent dye molecules to each binding element provides the multiplexed signal. In another embodiment, adding only one signal compound to each binding element provides the multiplexed signal, such that some of the binding elements have a different signal compound added. The different signal compounds provide the multiplexed signal.

    Abstract translation: 一种标记方法，用标记探针标记对象或对象的特定特征，提供可通过频谱分解进行分析的多路复用信号。 这种二进制和更高的编码方案可以用来标记生物细胞的组分。 在每种编码方案中，需要选择性结合特定特征的标记探针。 标记的探针包括与特征结合的结合元件和至少一个产生可检测信号的信号组分，优选为光谱标记。 在一个实施方案中，向每个结合元件添加多个荧光染料分子提供了多路复用信号。 在另一个实施方案中，仅向每个结合元件添加一种信号化合物提供了多路复用信号，使得一些结合元件具有添加的不同信号化合物。 不同的信号复合提供复用信号。

    TDI IMAGING SYSTEM FOR KINETIC STUDIES
    8.
    发明申请
    TDI IMAGING SYSTEM FOR KINETIC STUDIES 审中-公开
    TDI成像系统的动力学研究

    公开(公告)号：WO0216894A8

    公开(公告)日：2002-07-04

    申请号：PCT/US0126486

    申请日：2001-08-24

    Applicant: AMNIS CORP

    Inventor： BASIJI DAVID ,  ORTYN WILLIAM

    IPC: G01J3/28 ,  G01N15/14 ,  G01N21/47 ,  G01J3/14 ,  G01J3/443 ,  G01N21/64

    CPC classification number: G01N15/14 ,  G01J3/2803 ,  G01J3/2823 ,  G01N21/4795

    Abstract: Light from an object (24) such as a cell moving through an imaging system is collected, and imaged onto a time delay integration (TDI) detector (44), producing a pixeled output signal in response to the image of the object (24). The light can be emitted from a luminous object, from a source and scattered by the object, or can be a fluorescent emission by one or more object probes. Light absorbed or reflacted by the object can also produce images for determining specific characteristics of the objects. In one set of embodiments, the movement of the object is synchronized with that of the pixelated output signal, which is controlled by the readout rate of the TDI detector (44). Alternatively, the readout rate of the pixelated output signal is not synchronized with the movement of the object, thereby permitting multiple signals to be produced for each of a plurality of objects over time.

    Abstract translation: 收集来自诸如移动通过成像系统的单元的物体（24）的光，并将其成像到时间延迟积分（TDI）检测器（44）上，响应于物体（24）的图像产生像素输出信号， 。 光可以从发光物体发射，从源发射并被物体散射，或者可以是由一个或多个物体探针的荧光发射。 被物体吸收或反射的光也可以产生用于确定物体的特定特征的图像。 在一组实施例中，对象的移动与由像素化输出信号的移动同步，其由TDI检测器（44）的读出速率来控制。 或者，像素化输出信号的读出速率与对象的移动不同步，从而允许针对多个对象中的每一个随时间生成多个信号。

    CELL SUSPENSION ROTATING FLUIDIC PUMP
    9.
    发明申请
    CELL SUSPENSION ROTATING FLUIDIC PUMP 审中-公开
    细胞悬浮液旋转液体泵

    公开(公告)号：WO2004065544A3

    公开(公告)日：2005-05-12

    申请号：PCT/US2004001058

    申请日：2004-01-15

    Applicant: AMNIS CORP ,  HARUI NORIO ,  CRAWFORD MICHAEL ,  ESPOSITO RICHARD ,  ORTYN WILLIAM

    Inventor： HARUI NORIO ,  CRAWFORD MICHAEL ,  ESPOSITO RICHARD ,  ORTYN WILLIAM

    IPC: A61M5/00 ,  B65B1/04 ,  C12N20060101 ,  F04B9/02 ,  F04B13/00 ,  G01N15/14 ,  G01N35/10

    CPC classification number: G01N35/1095 ,  F04B9/02 ,  F04B13/00 ,  G01N15/1404 ,  G01N2015/1413 ,  Y10T436/11

    Abstract: A low pulsatility syringe pump including a duplex bearing set (218) rotatingly supporting a lead screw (216), and a transmission having a first drive train configured to increase a number of motor rotations required for a single rotation of the lead screw (216), and a second drive train configured to reduce the number of motor rotations required for a single rotation of the lead screw (216) as compared to the first drive train. Another embodiment also includes a motor (316) configured to rotate the syringe about its own axis, independent of the motion of the lead screw (216). In this other embodiment, the fluid in the syringe barrel includes objects (such as cells, latex beads, etc.) entrained in the fluid. The rate of rotation (e.g., about three revolutions per second) is chosen such that each object traces a substantially circular pathway in the syringe barrel and remains in suspension.

    Abstract translation: 一种低脉动注射器泵，包括旋转地支撑导螺杆（216）的双轴承套（218）和具有第一传动系的变速器，所述第一传动系被配置为增加所述丝杠（216）的单次旋转所需的马达转数， 以及配置成与第一传动系相比减少导螺杆（216）的单次旋转所需的马达转数的第二传动系。 另一个实施例还包括电动机（316），其构造成使注射器围绕其自身的轴线旋转，而与导螺杆（216）的运动无关。 在该另一个实施例中，注射器筒中的流体包括夹带在流体中的物体（例如细胞，乳胶珠等）。 选择旋转速率（例如，每秒约三转），使得每个物体在注射器筒中追踪基本上圆形的路径并保持悬浮状态。

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