公开(公告)号：EP4158063A1

公开(公告)日：2023-04-05

申请号：EP21812518.5

申请日：2021-05-28

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： LINDSAY, Stuart

IPC: C12Q1/6869 ,  C12Q1/6874 ,  G01N27/327 ,  G01N33/487

公开(公告)号：EP4143339A1

公开(公告)日：2023-03-08

申请号：EP21796212.5

申请日：2021-04-30

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： LINDSAY, Stuart

IPC: C12Q1/6869 ,  G01N27/00 ,  C07H21/04

公开(公告)号：WO2020257654A1

公开(公告)日：2020-12-24

申请号：PCT/US2020/038740

申请日：2020-06-19

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： LINDSAY, Stuart ,  ZHANG, Bintian ,  DENG, Hanqing

IPC: C07H21/04 ,  C12Q1/68 ,  C12N9/12 ,  C12Q1/6869 ,  G01N33/487

Abstract: Disclosed are devices, systems and methods for direct measurement of polymerase activity. In one example, a device includes at least a first electrode and a second electrode, the first and second electrode being separated by a gap; and a polymerase with two attachment sites, one for attaching to the first electrode and a second for attaching to the second electrode, wherein the two attachment sites are separated by a distance of at least about 1 nm and the distance does not significantly change with conformational changes of the polymerase.

公开(公告)号：EP4103940A1

公开(公告)日：2022-12-21

申请号：EP21754507.8

申请日：2021-02-11

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： LINDSAY, Stuart ,  SADAR, Joshua ,  QING, Quan

IPC: G01N27/30 ,  C12Q1/00 ,  G01N33/00

公开(公告)号：EP3986908A1

公开(公告)日：2022-04-27

申请号：EP20827063.7

申请日：2020-06-19

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： LINDSAY, Stuart ,  ZHANG, Bintian ,  DENG, Hanqing

IPC: C07H21/04 ,  C12Q1/68 ,  C12N9/12 ,  C12Q1/6869 ,  G01N33/487

公开(公告)号：WO2021211950A1

公开(公告)日：2021-10-21

申请号：PCT/US2021/027650

申请日：2021-04-16

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： LINDSAY, Stuart ,  ANDERSON, Karen ,  KNAPPENBERGER, Mark ,  AFSARI MAMAGHANI, Sepideh

IPC: C12Q1/6825 ,  C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides devices, systems, and methods related to single molecule detection. In particular, the present disclosure provides devices and methods for sequence-specific detection of a nucleic acid target using current fluctuations as a readout for protein binding to the nucleic acid target. As described herein, certain aspects of the bioelectronic devices and method can be used to detect and identify any nucleic acid target for the purpose of diagnosis and/or treatment.

公开(公告)号：WO2016077263A1

公开(公告)日：2016-05-19

申请号：PCT/US2015/059812

申请日：2015-11-10

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： TAKULAPALLI, Bharath ,  LINDSAY, Stuart

IPC: C12N15/09 ,  C12M1/00 ,  G01N21/00 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: The present invention provides a system and method of sequencing complex molecules including DNA, RNA, proteins, and glycans. The method includes the steps of modifying a field effect nanopore transistor device with chemical recognition molecules, translocating the complex molecule into the field effect nanopore transistor device, applying bias potential to the silicon gate of the field effect nanopore transistor device, and measuring the resulting change in drain current across the source drain contacts.

Abstract translation: 本发明提供了对包括DNA，RNA，蛋白质和聚糖在内的复合分子进行测序的系统和方法。 该方法包括以下步骤：用化学识别分子修饰场效应纳米孔晶体管器件，将复合分子转移到场效应纳米孔晶体管器件中，向场效应纳米孔晶体管器件的硅栅极施加偏置电位，并测量所得到的变化 在源极漏极接触中的漏极电流。

公开(公告)号：WO2022260963A1

公开(公告)日：2022-12-15

申请号：PCT/US2022/032211

申请日：2022-06-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： LINDSAY, Stuart ,  RYAN, Eathen ,  ZHANG, Bintian ,  WANG, Xu

IPC: C12Q1/00

Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure relates to methods for sequencing a polynucleotide using a bioelectronic device that includes protein assemblies used as coupling molecules in bioelectronic circuits. The present disclosure also provides multimeric protein assemblies with various combinations of live and dead subunits arranged to maximize conduction.

公开(公告)号：WO2022133113A1

公开(公告)日：2022-06-23

申请号：PCT/US2021/063851

申请日：2021-12-16

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： LINDSAY, Stuart ,  RYAN, Eathen

IPC: G01N27/00

Abstract: The present disclosure provides devices, systems, and methods related to protein bioelectronics. In particular, the present disclosure provides devices, systems, and methods for forming electrical contacts to a protein with high yield, which facilitates the manufacture of analytical devices to detect and measure the electrical characteristics corresponding to protein function.

公开(公告)号：WO2021222791A1

公开(公告)日：2021-11-04

申请号：PCT/US2021/030239

申请日：2021-04-30

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： LINDSAY, Stuart

IPC: C12Q1/6869 ,  G01N27/00 ,  C07H21/04 ,  G01N27/04 ,  G01N33/4833

Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure relates to methods for sequencing a polynucleotide using a bioelectronic device that obtains a bioelectronic signature (e.g., current amplitude levels) of polymerase activity based on current fluctuations as complementary nucleotidepolyphosphate monomers (e.g., having distinct charges) are incorporated into the template polynucleotide.