Abstract:
PROBLEM TO BE SOLVED: To provide a method, apparatus, and system for separating blood and other types of cellular components.SOLUTION: One of the exemplary methods includes: providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps 200 may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.
Abstract:
PROBLEM TO BE SOLVED: To provide a method, apparatus and system for separating blood and other types of cellular components, and which can be combined with holographic optical trapping manipulation or other forms of optical tweezing. SOLUTION: One of the exemplary methods includes: providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. COPYRIGHT: (C)2011,JPO&INPIT
Abstract:
Un aparato de clasificación de partículas, que comprende: una celda de flujo que presenta un canal de entrada de fluido (300) para introducir una muestra de partículas en unflujo laminar; dos canales de salida (302, 303) dispuestos a ambos lados de dicho canal de entrada de fluido (301), siendoadecuados cada uno de dichos dos canales de salida (302, 303) para hacer fluir a través de los mismos unadisolución amortiguadora y siendo adecuados cada uno de dichos dos canales de salida (302, 303) para introducirdicha disolución amortiguadora en dichos dos canales de salida (302, 303) a caudales que mantienen un flujolaminar con dicho flujo laminar en dicho canal de entrada de fluido (301); una región de clasificación que no tiene ninguna separación mecánica entre dicho canal de entrada de fluido (301)y dichos dos canales de salida (302, 303) y que tiene dichos flujos laminares en la misma; un sistema de captura óptica configurado para producir una formación en embudo de trampas ópticas (305) endicho canal de entrada de fluido cerca de dicha región de clasificación, estando configuradas dichas trampasópticas (305) para atrapar dichas partículas en dicho canal de entrada de fluido (301) y para separar dichaspartículas en la región de clasificación; en el que dicho sistema de captura óptica incluye una lente de objetivo de alta apertura numérica (304) configuradapara implementar dicha formación en embudo de trampas ópticas (305); y un sistema de detección configurado para detectar dichas partículas atrapadas para determinar las partículas quevan a separarse en dicha región de clasificación.
Abstract:
A method and apparatus for manipulating particles (micro, nano, and pico) having one or more characteristics with an optical trap formed by modulating a laser beam with a Diffractive Optical Element (DOE). At least one characteristic of the material is selected; and a laser beam having a selected wavelength corresponding to the at least one selected characteristic of the material is generated. Values of the DOE are calculated corresponding to the least one selected characteristic of the material. The beam and the DOE are modulated to produce a holographic optical trap having properties corresponding to the at least one selected characteristic; the trap is focused to a beam focus or selected spot size; and the beam focus is located near a particle location for trapping the particle therein.
Abstract:
Micrometer and nanometer-sized tools (69), referred to as MOTS an d NOTS, respectively, are manipulated in the illumination (71) of an optical trap (70) and are able to alter the physical, chemical or electronic structure or orientation of a workpiece (68).
Abstract:
The present invention employs a beam steering apparatus to isolate valuable cells from others cells, tissues, and contaminants. In one embodiment, the system balances optical trapping agains flow to parallelize cell sorting und er the flexible control of computer program-directed traps which differentially manipulate cells based on their composition or labels to direct separation.
Abstract:
A method for trapping particles where a characteristic of a particle is selected, a laser is used to generate a holographic optical trap, and the beams are modulated and focused as desired.