中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

20 records (took 0.02 seconds)

    TIGHT CONTROL OF GENE EXPRESSION IN EUCARYOTIC CELLS BY TETRACYCLINE-RESPONSIVE PROMOTERS
    1.
    发明申请
    TIGHT CONTROL OF GENE EXPRESSION IN EUCARYOTIC CELLS BY TETRACYCLINE-RESPONSIVE PROMOTERS 审中-公开
    通过四环素反应促进剂对真核细胞中基因表达的严格控制

    公开(公告)号：WO9429442A3

    公开(公告)日：1995-02-02

    申请号：PCT/US9406734

    申请日：1994-06-14

    Applicant: BASF AG ,  BUJARD HERMANN ,  GOSSEN MANFRED ,  SALFELD JOCHEN G ,  VOSS JEFFREY W

    Inventor： BUJARD HERMANN ,  GOSSEN MANFRED ,  SALFELD JOCHEN G ,  VOSS JEFFREY W

    IPC: A01K67/027 ,  A61K48/00 ,  C07K14/00 ,  C12N5/08 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/31 ,  C12N15/62 ,  C12N15/63 ,  C12N15/85 ,  C12R1/91 ,  C12N1/15 ,  C12N1/19 ,  C12N15/12 ,  C12N15/90

    CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/09 ,  C07K2319/61 ,  C07K2319/71 ,  C07K2319/80 ,  C12N15/62 ,  C12N15/635 ,  C12N15/85 ,  C12N2830/006

    Abstract: Transgenic animals carrying two transgenes, the first coding for a transactivator fusion protein comprising a tet repressor and a polypeptide which directly or indirectly activates in eucaryotic cells, and the second comprising a gene operably linked to a minimal promotor operably linked to at least one tet operator sequence, are disclosed. Isolated DNA molecules (e.g., targeting vectors) for integrating a polynucleotide sequence encoding a transactivator of the invention at a predetermined location within a second target DNA molecule by homologous recombination are also disclosed. Transgenic animals having the DNA molecules of the invention integrated at a predetermined location in a chromosome by homologous recombination are also encompassed by the invention. Methods to regulate the expression of a tet operator linked gene of interest by administering tetracycline or a tetracycline analogue to an animal of the invention are also disclosed. The regulatory system of the invention allows for conditional inactivation or modulation of expression of a gene of interest in a host cell or animal.

    Abstract translation: 携带两个转基因的转基因动物，第一编码包含直接或间接在真核细胞中活化的tet阻遏物和多肽的反式激活因子融合蛋白，第二编码包含与可操作地连接至少一个tet操纵子的最小启动子可操作地连接的基因 序列。 还公开了通过同源重组将编码本发明的反式激活物的多核苷酸序列整合到第二靶DNA分子内的预定位置的分离的DNA分子（例如靶向载体）。 通过同源重组将具有整合在染色体中预定位置的本发明的DNA分子的转基因动物也包括在本发明中。 还公开了通过向本发明的动物施用四环素或四环素类似物来调节所关注的tet操纵子连接基因的表达的方法。 本发明的调节系统允许在宿主细胞或动物中有条件地失活或调控感兴趣的基因的表达。

    TETRACYCLINE-REGULATED TRANSCRIPTIONAL MODULATORS
    4.
    发明专利
    TETRACYCLINE-REGULATED TRANSCRIPTIONAL MODULATORS 未知

    公开(公告)号：CA2193122C

    公开(公告)日：2005-08-23

    申请号：CA2193122

    申请日：1995-06-29

    Applicant: BASF AG ,  KNOLL AG

    Inventor： BUJARD HERMANN ,  GOSSEN MANFRED

    IPC: A01K67/027 ,  A61K48/00 ,  C07K14/00 ,  C07K14/035 ,  C07K14/195 ,  C07K14/245 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N15/09 ,  C12N15/11 ,  C12N15/12 ,  C12N15/62 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85 ,  C12N15/79 ,  A01H5/00 ,  A61K31/70 ,  C12N15/31 ,  C12N15/38

    Abstract: Nucleic acid molecules and proteins useful for regulating the expression of genes in eukaryotic cells and organisms are disclosed. Theinvention provides a transcriptional activator fusion protein which binds ro tet operator sequences and stimuiares transcription of a let operator-linked gene in the presence but not the absence, of tetracycline (o r analogue thereof). The invention further provides transcriptional inhibitor fusion proteins which inhibit transcription of a let operaror-(ink ed gene in a regulated manner. In one embodiment, the inhibitor fusion protein hinds to tet operator sequences in the absence, but not the presence, of tetracycline (or analogue). In another embodiment, the inhibitor fusion protein binds to tet operator sequences in the presence, bu t not the absence, of tetracycline (or analogue). The transcriptional activator and inhibitor fusion proteins of the invention can be used in combination to regulate expression of one or multiple tet operator-linked genes. Novel tet operator-containing transcription units whi ch allow for coordinate or independent tetracycline-regulated expression of two or more genes by the transcriptional modulators of the invention are also disclosed. Kits including the components of the regulatory system are also encompassed by the invention.

    Tetracycline-regulated transcriptional modulators
    5.
    发明专利
    Tetracycline-regulated transcriptional modulators 未知

    公开(公告)号：AU3092395A

    公开(公告)日：1996-01-25

    申请号：AU3092395

    申请日：1995-06-29

    Applicant: BASF AG ,  KNOLL AG

    Inventor： BUJARD HERMANN ,  GOSSEN MANFRED

    IPC: A01K67/027 ,  A61K48/00 ,  C07K14/00 ,  C07K14/035 ,  C07K14/195 ,  C07K14/245 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N15/09 ,  C12N15/11 ,  C12N15/12 ,  C12N15/62 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85 ,  C12N15/00

    Abstract: Nucleic acid molecules and proteins useful for regulating the expression of genes in eukaryotic cells and organisms are disclosed. The invention provides a transcriptional activator fusion protein which binds to tet operator sequences and stimulates transcription of a tet operator-linked gene in the presence, but not the absence, of tetracycline (or analogue thereof). The invention further provides transcriptional inhibitor fusion proteins which inhibit transcription of a tet operator-linked gene in a regulated manner. In one embodiment, the inhibitor fusion protein binds to tet operator sequences in the absence, but not the presence, of tetracycline (or analogue). In another embodiment, the inhibitor fusion protein binds to tet operator sequences in the presence, but not the absence, of tetracycline (or analogue). The transcriptional activator and inhibitor fusion proteins of the invention can be used in combination to regulate expression of one or multiple tet operator-linked genes. Novel tet operator-containing transcription units which allow for coordinate or independent tetracycline-regulated expression of two or more genes by the transcriptional modulators of the invention are also disclosed. Kits including the components of the regulatory system are also encompassed by the invention.

    TETRACYCLINE-REGULATED TRANSCRIPTIONAL MODULATORS
    6.
    发明专利
    TETRACYCLINE-REGULATED TRANSCRIPTIONAL MODULATORS 未知

    公开(公告)号：CA2193122A1

    公开(公告)日：1996-01-18

    申请号：CA2193122

    申请日：1995-06-29

    Applicant: BASF AG ,  KNOLL AG

    Inventor： BUJARD HERMANN ,  GOSSEN MANFRED

    IPC: A01K67/027 ,  A61K48/00 ,  C07K14/00 ,  C07K14/035 ,  C07K14/195 ,  C07K14/245 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N15/09 ,  C12N15/11 ,  C12N15/12 ,  C12N15/62 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85 ,  C12N15/79 ,  A01H5/00 ,  A61K31/70 ,  C12N15/31 ,  C12N15/38

    Abstract: Nucleic acid molecules and proteins useful for regulating the expression of genes in eukaryotic cells and organisms are disclosed. Theinvention provides a transcriptional activator fusion protein which binds to tet operator sequences and stimulates transcription of a tet operator-linked gene in the presence, but not the absence, of tetracycline (or analogue thereof). The invention further provides transcriptional inhibitor fusion proteins which inhibit transcription of a tet operator-linked gene in a regulated manner. In one embodiment, the inhibitor fusion protein binds to tet operator sequences in the absence, but not the presence, of tetracycline (or analogue). In another embodiment, the inhibitor fusion protein binds to tet operator sequences in the presence, but not the absence, of tetracycline (or analogue). The transcriptional activator and inhibitor fusion proteins of the invention can be used in combination to regulate expression of one or multiple tet operator-linked genes. Novel tet operatorcontaining transcription units which allow for coordinate or independent tetracycline-regulated expression of two or more genes by the transcriptional modulators of the invention are also disclosed. Kits including the components of the regulatory system are also encompassed by the invention.

Patent Agency Ranking