公开(公告)号：EP2678421A4

公开(公告)日：2015-08-19

申请号：EP12749577

申请日：2012-02-20

Applicant: BASF SE

Inventor： WITTMANN CHRISTOPH ,  KIND STEFANIE

IPC: C12N9/88 ,  C12P13/00 ,  C12R1/15

CPC classification number: C12P13/02 ,  C07K14/34 ,  C12N9/88 ,  C12N15/77 ,  C12P13/001

Abstract: Recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine or N-acetylcadaverine, as well as recombinant DNA molecules and polypeptides used to produce the microorganisms are provided. Said microorganisms comprise an intracellular lysine decarboxylase activity and a deregulated cadaverine export activity, or comprise a decreased cadaverine export activity and an enhanced N-acetylcadaverine forming activity. Processes for the production of cadaverine N-acetylcadaverine using the recombinant microorganisms are also provided.

公开(公告)号：EP2794852A4

公开(公告)日：2015-09-02

申请号：EP12859116

申请日：2012-12-14

Applicant: BASF SE

Inventor： WITTMANN CHRISTOPH ,  KIND STEFANIE ,  BECKER JUDITH

IPC: C12N1/00 ,  C12N15/52

CPC classification number: C12P13/08 ,  C12N9/93 ,  C12P13/001 ,  C12Y602/01004 ,  C12Y602/01005 ,  Y02P20/52

Abstract: Recombinant microorganisms with deregulated succinyl-CoA synthetase activity, as well as the uses for producing lysine, β-lysine, cadaverine or N-acetylcadaverine thereby are provided. Recombinant polypeptides comprising an amino acid sequence being at least 80% identical to SEQ ID NO: 51 or 53 are also provided. The method of producing fine chemicals using said recombinant microorganisms, in particular the method of producing lysine, or derivatives thereof, such as β-lysine, cadaverine or N-acetylcadaverine is further provided.

Abstract translation: 重组微生物具有失调的琥珀酰辅酶A合成酶活性，以及​​作为制造赖氨酸，β-赖氨酸，尸胺或N-乙酰尸胺的用途，由此提供。 重组多肽，其包含的氨基酸序列有至少80％相同于SEQ ID NO：提供51或53因此。 还提供了使用所述重组微生物生产精细化学品，尤其是生产赖氨酸的方法，或它们的衍生物，：例如β-赖氨酸，尸胺或N-乙酰尸胺的方法。