公开(公告)号：AU2012339759B2

公开(公告)日：2018-06-21

申请号：AU2012339759

申请日：2012-11-13

Applicant: BASF SE

Inventor： NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

IPC: C12N9/88

Abstract: The present invention relates to methods of producing, isolating, identifying and characterizing herbicide tolerant ACCase variants. In one embodiment, the invention encompasses a method of screening for an acetyle-CoA carboxylase (ACCase) enzyme which is tolerant to at least one herbicide, comprising: a) providing an ACCase-deficient yeast with a chimeric ACCase, said chimeric ACCase comprising: at least two regions that further comprise: i) an N-terminal region, said N-terminal region derived from yeast, fungi or monocot cytoplasmic ACCases, preferably a yeast ACCase; ii) a C-terminal region, said C-terminal region derived from monocot plastific ACCases and comprising an HSR; and iii) said N-terminal region comprises about 50% to about 60% of the chimeric ACCase; b) isolating herbicide tolerant yeast cells after culturing in the presence of at least one herbicide; and c) further comprising identifying the mutation(s) not present in chimeric ACCase prior to culturing, which confers tolerance to at least one herbicide.

公开(公告)号：CA2855933C

公开(公告)日：2020-08-04

申请号：CA2855933

申请日：2012-11-13

Applicant: BASF SE ,  NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

Inventor： NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

IPC: C12N9/00 ,  C12N15/52 ,  C12N15/81

Abstract: The present invention relates to methods of producing, isolating, identifying and characterizing herbicide tolerant ACCase variants. In one embodiment, the invention encompasses a method of screening for an acetyle-CoA carboxylase (ACCase) enzyme which is tolerant to at least one herbicide, comprising: a) providing an ACCase-deficient yeast with a chimeric ACCase, said chimeric ACCase comprising: at least two regions that further comprise: i) an N-terminal region, said N-terminal region derived from yeast, fungi or monocot cytoplasmic ACCases, preferably a yeast ACCase; ii) a C-terminal region, said C-terminal region derived from monocot plastific ACCases and comprising an HSR; and iii) said N-terminal region comprises about 50% to about 60% of the chimeric ACCase; b) isolating herbicide tolerant yeast cells after culturing in the presence of at least one herbicide; and c) further comprising identifying the mutation(s) not present in chimeric ACCase prior to culturing, which confers tolerance to at least one herbicide.

公开(公告)号：AU2012339759A1

公开(公告)日：2014-06-05

申请号：AU2012339759

申请日：2012-11-13

Applicant: STEVENSON PAULIK JILL ,  BASF SE ,  MANKIN SCOTTS ,  MCELVER JOHN ,  NEUTEBOOM LEON ,  WITT SHERRY

Inventor： NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

IPC: C12N9/88

Abstract: The present invention relates to methods of producing, isolating, identifying and characterizing herbicide tolerant ACCase variants. In one embodiment, the invention encompasses a method of screening for an acetyle-CoA carboxylase (ACCase) enzyme which is tolerant to at least one herbicide, comprising: a) providing an ACCase-deficient yeast with a chimeric ACCase, said chimeric ACCase comprising: at least two regions that further comprise: i) an N-terminal region, said N-terminal region derived from yeast, fungi or monocot cytoplasmic ACCases, preferably a yeast ACCase; ii) a C-terminal region, said C-terminal region derived from monocot plastific ACCases and comprising an HSR; and iii) said N-terminal region comprises about 50% to about 60% of the chimeric ACCase; b) isolating herbicide tolerant yeast cells after culturing in the presence of at least one herbicide; and c) further comprising identifying the mutation(s) not present in chimeric ACCase prior to culturing, which confers tolerance to at least one herbicide.

公开(公告)号：CA2855933A1

公开(公告)日：2013-05-23

申请号：CA2855933

申请日：2012-11-13

Applicant: BASF SE ,  NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

Inventor： NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

IPC: C12N9/00 ,  C12N15/52 ,  C12N15/81

Abstract: The present invention relates to methods of producing, isolating, identifying and characterizing herbicide tolerant ACCase variants. In one embodiment, the invention encompasses a method of screening for an acetyle-CoA carboxylase (ACCase) enzyme which is tolerant to at least one herbicide, comprising: a) providing an ACCase-deficient yeast with a chimeric ACCase, said chimeric ACCase comprising: at least two regions that further comprise: i) an N-terminal region, said N-terminal region derived from yeast, fungi or monocot cytoplasmic ACCases, preferably a yeast ACCase; ii) a C-terminal region, said C-terminal region derived from monocot plastific ACCases and comprising an HSR; and iii) said N-terminal region comprises about 50% to about 60% of the chimeric ACCase; b) isolating herbicide tolerant yeast cells after culturing in the presence of at least one herbicide; and c) further comprising identifying the mutation(s) not present in chimeric ACCase prior to culturing, which confers tolerance to at least one herbicide.

公开(公告)号：EP2788482A4

公开(公告)日：2015-08-05

申请号：EP12850161

申请日：2012-11-13

Applicant: BASF SE ,  NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

Inventor： NEUTEBOOM LEON ,  WITT SHERRY R ,  MCELVER JOHN ,  STEVENSON-PAULIK JILL M ,  MANKIN SCOTTS L

IPC: C12N9/88 ,  C12N9/00 ,  C12N15/82

CPC classification number: C12N9/93 ,  C12N15/8274 ,  C12Y604/01 ,  C12Y604/01002

Abstract: The present invention relates compositions and methods for identifying, isolating, and characterizing herbicide tolerant mutations in monocot plastidic acetyl-CoA carboxylases using a model system.