Abstract:
PROBLEM TO BE SOLVED: To provide sunflower plants having increased resistance to herbicides when compared to a wild-type sunflower plant.SOLUTION: A sunflower plant comprises in its genome at least one copy of at least one acetohydroxyacid synthase large subunit (AHASL) polynucleotide, wherein the AHASL polynucleotide comprises (a) an amino acid sequence set forth in a different sequence having a threonine at a specific position of an amino acid sequence set forth in a specific sequence or equivalent to the position.
Abstract:
Un procedimiento de producción de una enzima acetil-CoA carboxilasa (ACCasa) que es tolerante a al menos un herbicida, que comprende: a. proporcionar una levadura deficiente en ACCasa que comprende un ácido nucleico que codifica una ACCasa quimérica, comprendiendo dicha ACCasa quimérica una región N-terminal y una región Cterminal, en el que la región C-terminal comprende una región de sensibilidad a herbicidas (HSR) de una ACCasa plastídica de monocotiledónea de una Poaceae; b. poner en contacto dicha levadura con al menos un oligonucleótido mutagénico en condiciones que permitan la mutagénesis dirigida al sitio de al menos un codón del ácido nucleico que codifica la ACCasa quimérica, y hacer crecer la levadura mutagenizada formando de este modo una biblioteca de colonias de levadura mutagenizadas; c. cultivar las colonias de levadura mutagenizadas en presencia de al menos un herbicida inhibidor de ACCasa para formar colonias tratadas; y d. aislar de al menos una de dichas colonias tratadas, al menos una levadura mutagenizada que crece en presencia del herbicida, en el que la levadura mutagenizada que crece en presencia del herbicida comprende una ACCasa mutagenizada que tiene una tolerancia al herbicida que es mayor que la exhibida por la ACCasa quimérica de la etapa (a): en el que el procedimiento es un procedimiento de alto rendimiento en el que un trabajador capacitado puede generar y seleccionar al menos 500 diferentes ACCasas con HSR variante por mes, cada una de su propia colonia celular.
Abstract:
Abstract Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide-resistant and wild-type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.
Abstract:
Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide-resistant and wild-type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.
Abstract:
Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide-resistant and wild-type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.
Abstract:
Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide resistant and wild type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformer with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.
Abstract:
Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide resistant and wild type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformer with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.