公开(公告)号：WO9806823A3

公开(公告)日：1998-05-07

申请号：PCT/US9713759

申请日：1997-08-13

Applicant: BAXTER INT

Inventor： VACHULA MONA ,  VAN EPPS DENNIS E ,  ALZONA MORTIMER T ,  AONO FREDERICK M

IPC: A61K38/00 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/06

CPC classification number: C12N5/0636 ,  A61K38/00 ,  A61K2039/5154 ,  A61K2039/5158 ,  C12N5/0639 ,  C12N2500/05 ,  C12N2500/14 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/02 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/25 ,  C12N2501/515

Abstract: A method for producing human dendritic cells for therapeutic purposes which allows culture-deriving dendritic cells using no cytokines, or reduced cytokines. The method involves culturing mononuclear cells from blood or bone marrow in a medium containing at least one agent such as a calcium ionophore, e.g. A23187, theophylline, protaglandin E1, dibutyryl cyclic AMP, Vitamin D3, Vitamin E, retinoic acid, or a fatty acid. The culture is maintained for a sufficient time, typically 4 - 14 days, to produce a culture enriched for dendritic cells, as evidenced by at least about 2.5 % of total cells exhibiting dendritic cell processes, or a dendritic cell antigen such as CD80, CD86, or CD1a. Also provided is a method to produce antigen-specific human T-cells by pulsing the dendritic cells obtained by the method of the invention with an antigen such as a viral, tumor, bacterial, or cell surface antigen, and then co-culturing T-cells with the antigen-pulsed dendritic cells. Useful for treatment of viral or bacterial infections, useful as a cancer vaccine, useful to induce tolerance of allo- or xeno-graft.

Abstract translation: 用于制备用于治疗目的的人树突状细胞的方法，其允许不使用细胞因子的培养物衍生的树突状细胞或减少的细胞因子。 该方法包括在含有至少一种如钙离子载体的试剂的培养基中从血液或骨髓中培养单核细胞。 A23187，茶碱，protaglandin E1，二丁酰环AMP，维生素D3，维生素E，视黄酸或脂肪酸。 培养物保持足够的时间，通常为4-14天，以产生富含树突状细胞的培养物，如通过表达树突状细胞过程的总细胞的至少约2.5％或树突状细胞抗原如CD80，CD86所证明的 ，或CD1a。 还提供了通过用抗原如病毒，细胞或细胞表面抗原脉冲通过本发明的方法获得的树突细胞产生抗原特异性人T细胞的方法，然后共培养T- 细胞与抗原脉冲的树突状细胞。 可用于治疗病毒或细菌感染，可用作癌症疫苗，可用于诱导同种异体或异种移植物的耐受性。

公开(公告)号：AU4052197A

公开(公告)日：1998-03-06

申请号：AU4052197

申请日：1997-08-13

Applicant: BAXTER INT

Inventor： VACHULA MONA ,  EPPS DENNIS E VAN ,  ALZONA MORTIMER T ,  AONO FREDERICK M

IPC: A61K38/00 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/06

公开(公告)号：AU4069097A

公开(公告)日：1998-03-06

申请号：AU4069097

申请日：1997-08-13

Applicant: BAXTER INT

Inventor： VACHULA MONA ,  ALZONA MORTIMER T ,  AONO FREDERICK M ,  KOWALKOWSKI KENNETH L ,  SMITH STEPHEN L ,  UNVERZAGT KRISTEN L ,  EPPS DENNIS E VAN

IPC: A61K38/00 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/08 ,  A61K35/14 ,  A61K35/28