公开(公告)号：WO1988007668A1

公开(公告)日：1988-10-06

申请号：PCT/US1988001046

申请日：1988-03-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HOLMQUIST, Barton ,  SCOPES, Robert, K.

IPC: G01N01/28

CPC classification number: B01F13/0016 ,  B01F3/08 ,  G01N1/38

Abstract: This invention relates to a diluting and mixing device which is capable of diluting a first solution to produce a second solution which is mixed with an undiluted third solution, to reproducibly produce a unique series of combined solutions. Each solution in said series of combined solutions may vary only in the concentration of a single (selected) reactant, and typically, each successive solution becomes increasingly more concentrated in the selected reactant. By employing a modification in procedure, each successive solution in said series may become decreasingly less concentrated in the selected reactant. This invention further relates to an automated system comprising the device connected to a stepping motor so as to rapidly and reproducibly produce said series of solutions, said device being further connected to an analyzer means for obtaining chemical, biochemical, or physical chemical data on said series of solutions. This invention especially relates to an embodiment of the device for obtaining kinetic data on enzyme reactions in solutions. The device is useful because it provides a means for rapidly obtaining chemical, biochemical, or physical chemical data on reactions in solution.

Abstract translation: 本发明涉及稀释和混合装置，其能够稀释第一溶液以产生与未稀释的第三溶液混合的第二溶液，以可重复地产生独特的一系列组合溶液。 所述一系列组合溶液中的每种溶液可以仅在单一（选择的）反应物的浓度上变化，并且通常，每个连续的溶液变得越来越多地浓缩在所选择的反应物中。 通过采用程序的改进，所述系列中的每个连续溶液可能在所选择的反应物中的浓度越来越少。 本发明还涉及一种自动化系统，其包括连接到步进电机的装置，以便快速且可重复地产生所述一系列解决方案，所述装置进一步连接到用于获得所述系列上的化学，生物化学或物理化学数据的分析装置 的解决方案。 本发明特别涉及用于获得溶液中酶反应动力学数据的装置的实施方案。 该设备是有用的，因为它提供了快速获得溶液中反应的化学，生物化学或物理化学数据的手段。

公开(公告)号：EP0310649B1

公开(公告)日：1992-09-30

申请号：EP88903679.4

申请日：1988-03-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： HOLMQUIST, Barton ,  SCOPES, Robert, K.

IPC: G01N1/28 ,  G01N35/08 ,  B01F3/08 ,  B01F13/00

CPC classification number: B01F13/0016 ,  B01F3/08 ,  G01N1/38

Abstract: This invention relates to a diluting and mixing device which is capable of diluting a first solution to produce a second solution which is mixed with an undiluted third solution, to reproducibly produce a unique series of combined solutions. Each solution in said series of combined solutions may vary only in the concentration of a single (selected) reactant, and typically, each successive solution becomes increasingly more concentrated in the selected reactant. By employing a modification in procedure, each successive solution in said series may become decreasingly less concentrated in the selected reactant. This invention further relates to an automated system comprising the device connected to a stepping motor so as to rapidly and reproducibly produce said series of solutions, said device being further connected to an analyzer means for obtaining chemical, biochemical, or physical chemical data on said series of solutions. This invention especially relates to an embodiment of the device for obtaining kinetic data on enzyme reactions in solutions. The device is useful because it provides a means for rapidly obtaining chemical, biochemical, or physical chemical data on reactions in solution.

公开(公告)号：EP0310649A1

公开(公告)日：1989-04-12

申请号：EP88903679.0

申请日：1988-03-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： HOLMQUIST, Barton ,  SCOPES, Robert, K.

IPC: G01N1 ,  B01F3 ,  B01F13 ,  G01N35

CPC classification number: B01F13/0016 ,  B01F3/08 ,  G01N1/38

Abstract: Dispositif de dilution et de mélange permettant de diluer une première solution pour produire une deuxième solution qui est mélangée avec une troisième solution non diluée, afin de produire de façon reproductible une série unique de solutions combinées. La concentration de chaque solution de la série peut varier par rapport à un réactant unique (choisi) et, en particulier, chaque solution présente une concentration croissante par rapport à ce réactant. En modifiant la procédure, chaque solution successive de la série peut présenter une concentration décroissante par rapport au réactant choisi. L'invention se rapporte également à un système automatisé comprenant ledit dispositif asservi à un moteur pas-à-pas, de façon à produire lesdites séries de solutions rapidement et de façon reproductible, ledit dispositif étant en outre relié à des moyens d'analyse permettant d'obtenir des données chimiques, biochimiques ou physico-chimiques concernant ladite série de solutions. L'invention concerne en particulier une forme d'exécution du dispositif permettant d'obtenir des données cinétiques sur les réactions enzymatiques en solution. Le dispositif s'avère utile car il permet d'obtenir rapidement des données chimiques, biochimiques ou physico-chimiques sur les réactions en solution.