公开(公告)号：WO1996029571A1

公开(公告)日：1996-09-26

申请号：PCT/US1996003841

申请日：1996-03-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KAYE, Wilbur ,  PENTONEY, Stephen, L., Jr.

IPC: G01B11/27

CPC classification number: G01N27/44782 ,  G01N21/65 ,  G01N27/44704 ,  G01N27/44721 ,  G01N30/74

Abstract: A system and method are disclosed for optically aligning a capillary tube and an excitation laser beam for fluorescence detection applications by utilizing the Raman scatter signals of the capillary tube's contents. For example, Raman scatter by an electrophoretic separation matrix may be used for alignment in a capillary electrophoresis system. Fluorescent material may be present and may also be used for alignment purposes, but is not necessary. The invention employs a parabolic reflector, having apertures through which the capillary tube and the laser beam are guided so that they intersect, preferably at right angles and at the focal point of the reflector. The Raman scatter signals of the material within the capillary tube are collected via a series of filters and this information is used to reposition, if necessary, a focusing lens that directs the excitation beam into the reflector and the capillary tube, so that the Raman scatter signals are maximized. Maximal Raman scatter signals indicate proper alignment of the capillary tube and the excitation beam. Other signals, such as fluorescence emission from the sample, may then be gathered. Adjustment of the focusing lens may be automated so that alignment of the capillary tube and the beam is maintained throughout analysis of the tube's contents. Sequential alignment of an array of capillary tubes with an excitation beam is also disclosed.

Abstract translation: 公开了一种用于通过利用毛细管内容物的拉曼散射信号将毛细管和激发激光束用于荧光检测应用的系统和方法。 例如，通过电泳分离基质的拉曼散射可以用于毛细管电泳系统中的比对。 荧光材料可以存在并且也可以用于对准目的，但不是必需的。 本发明采用抛物面反射器，其具有孔，毛细管和激光束通过该孔具有孔，使得它们优选地以直角相交，并在反射器的焦点处相交。 毛细管内的材料的拉曼散射信号通过一系列滤光片收集，并且该信息用于重新定位（如果需要的话）将激发光束引导到反射器和毛细管中的聚焦透镜，使得拉曼散射 信号最大化。 最大拉曼散射信号表示毛细管和激发光束的正确对准。 然后可以收集其它信号，例如来自样品的荧光发射。 聚焦透镜的调整可以是自动化的，以便在分析管的内容物期间保持毛细管和束的对准。 还公开了具有激发束的毛细管阵列的顺序对准。

公开(公告)号：EP0760933A1

公开(公告)日：1997-03-12

申请号：EP96910498.0

申请日：1996-03-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： KAYE, Wilbur ,  PENTONEY, Stephen, L., Jr.

IPC: G01B11 ,  G01N21 ,  G01N27 ,  G01N30

CPC classification number: G01N27/44782 ,  G01N21/65 ,  G01N27/44704 ,  G01N27/44721 ,  G01N30/74

Abstract: A system and method are disclosed for optically aligning a capillary tube and an excitation laser beam for fluorescence detection applications by utilizing the Raman scatter signals of the capillary tube's contents. For example, Raman scatter by an electrophoretic separation matrix may be used for alignment in a capillary electrophoresis system. Fluorescent material may be present and may also be used for alignment purposes, but is not necessary. The invention employs a parabolic reflector, having apertures through which the capillary tube and the laser beam are guided so that they intersect, preferably at right angles and at the focal point of the reflector. The Raman scatter signals of the material within the capillary tube are collected via a series of filters and this information is used to reposition, if necessary, a focusing lens that directs the excitation beam into the reflector and the capillary tube, so that the Raman scatter signals are maximized. Maximal Raman scatter signals indicate proper alignment of the capillary tube and the excitation beam. Other signals, such as fluorescence emission from the sample, may then be gathered. Adjustment of the focusing lens may be automated so that alignment of the capillary tube and the beam is maintained throughout analysis of the tube's contents. Sequential alignment of an array of capillary tubes with an excitation beam is also disclosed.