公开(公告)号：WO1998022509A1

公开(公告)日：1998-05-28

申请号：PCT/US1997020935

申请日：1997-11-17

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KUUS-REICHEL, Kristine ,  LINTON, Harry, Jay ,  PAYNE, Janice, K. ,  WANG, Tang, Jang

IPC: C07K16/40

CPC classification number: C12N9/6445 ,  C07K14/8121 ,  C07K16/38 ,  C07K16/40

Abstract: Monoclonal antibodies have been developed which specifically bind the PSA-ACT complex without significant cross-reactivity with PSA, ACT or CG-ACT. The antibodies were generated using a unique immunogen comprising a PSA-ACT complex having bound to monoclonal antibodies against PSA and monoclonal antibodies against ACT. It is theorized that the antibodies against PSA and ACT block immunodominant sites on the PSA-ACT complex. The antibodies generated against the unique immunogen specifically bind the PSA-ACT complex, but have no significant cross-reactivity with either PSA or ACT. The monoclonal antibodies of the present invention can be used in immunoassays to specifically detect PSA-ACT in a sample.

Abstract translation: 已经开发了单克隆抗体，其特异性结合PSA-ACT复合物，而与PSA，ACT或CG-ACT没有显着的交叉反应性。 使用独特的免疫原产生抗体，所述免疫原包含结合针对PSA的单克隆抗体和针对ACT的单克隆抗体的PSA-ACT复合物。 理论上，PSA和ACT抗体在PSA-ACT复合物上阻断免疫显性位点。 针对独特免疫原产生的抗体特异性结合PSA-ACT复合物，但与PSA或ACT无明显交叉反应。 本发明的单克隆抗体可用于免疫测定以特异性检测样品中的PSA-ACT。