公开(公告)号：WO1997032212A1

公开(公告)日：1997-09-04

申请号：PCT/US1997002748

申请日：1997-02-24

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OBREMSKI, Robert ,  SILZEL, John, W.

IPC: G01N33/543

CPC classification number: G01N21/648 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/54373

Abstract: A system for simultaneously conducting multiple ligand assays on a sample potentially containing target analytes uses as a detector a waveguide having thereon a plurality of probes of known recognition to the target analytes. The probes are in discrete areas on the waveguide. A sample containing target analyte is treated with a light-responsive compound such that it binds to the target analyte to form a conjugate and the conjugate is applied to the probes on the waveguide. A laser light is passed into the waveguide so that evanescent waves radiate from the waveguide. Where conjugate has attached to probe there is emission of light different from that emitted by a probe without conjugate attached thereto.

Abstract translation: 用于在潜在地含有目标分析物的样品上同时进行多个配体测定的系统用作其上具有已知识别到目标分析物的多个探针的检测器。 探针位于波导上的离散区域中。 用光响应化合物处理含有目标分析物的样品，使其与目标分析物结合形成共轭物，并将缀合物施加到波导上的探针上。 将激光传送到波导中，使得ev逝波从波导辐射。 当缀合物连接到探针上时，发射不同于由其连接的缀合物的探针发射的光。