公开(公告)号：WO1997008545A1

公开(公告)日：1997-03-06

申请号：PCT/US1996013165

申请日：1996-08-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  PENTONEY, Stephen, L., Jr. ,  PETERSON, Brian, D. ,  OSBORNE, James, C. ,  KEENAN, Charles, A.

IPC: G01N27/447

CPC classification number: G01N27/44704 ,  G01N27/44782

Abstract: A gel pump operated by a stepper motor may be used to deliver fresh gel to one or more capillaries through one or more manifolds and valves. By controlling the valves, fresh gel delivered by the pump will replace the old gel in the capillaries for an automated gel replacement system. In a different setting of the valves, the manifolds may be purged of the old gel prior to delivery of fresh gel to the capillaries. The gel delivery system may also be combined with an electrophoresis system so that sealing connection capable of withstanding high pressure adequate for gel injection need not be frequently broken when the gel is to be replaced. Manifold/reflector assembly is advantageously used in the system that facilitates electrical circuit for electrophoresis and for gel replacement.

Abstract translation: 由步进电动机操作的凝胶泵可以用于通过一个或多个歧管和阀将新鲜的凝胶递送到一个或多个毛细管。 通过控制阀门，由泵输送的新鲜凝胶将替代毛细管中的旧凝胶，用于自动凝胶置换系统。 在阀的不同设置中，在将新鲜凝胶递送至毛细管之前，歧管可以被清除旧凝胶。 凝胶递送系统还可以与电泳系统组合，使得当要更换凝胶时，不需要频繁地破坏能够耐受用于凝胶注射的高压的密封连接。 歧管/反射器组件有利地用于便于用于电泳和凝胶置换的电路的系统中。

公开(公告)号：WO1996030754A1

公开(公告)日：1996-10-03

申请号：PCT/US1996004321

申请日：1996-03-29

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  RAMPAL, Sushma ,  RAMPAL, Jang, B. ,  OSBORNE, James, C.

IPC: G01N27/447

CPC classification number: G01N27/44743

Abstract: A sample having both positively and negatively charged components may be separated during the same run by injecting a plug of sample at both ends of the capillary and applying an electric field in the capillary, where there is insufficient electroosmotic flow in the capillary to reverse sample migration direction, such as in the case of a gel-filled column.

Abstract translation: 具有正电荷和负电荷的组分的样品可以在相同的运行期间通过在毛细管的两端注入样品塞并在毛细管中施加电场，其中在毛细管中电渗流不足以反向样品迁移 方向，例如在凝胶填充柱的情况下。

公开(公告)号：EP0850412A1

公开(公告)日：1998-07-01

申请号：EP96928167.0

申请日：1996-08-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： PENTONEY, Stephen, L., Jr. ,  PETERSON, Brian, D. ,  OSBORNE, James, C. ,  KEENAN, Charles, A.

IPC: G01N27

CPC classification number: G01N27/44704 ,  G01N27/44782

Abstract: A gel pump operated by a stepper motor may be used to deliver fresh gel to one or more capillaries through one or more manifolds and valves. By controlling the valves, fresh gel delivered by the pump will replace the old gel in the capillaries for an automated gel replacement system. In a different setting of the valves, the manifolds may be purged of the old gel prior to delivery of fresh gel to the capillaries. The gel delivery system may also be combined with an electrophoresis system so that sealing connection capable of withstanding high pressure adequate for gel injection need not be frequently broken when the gel is to be replaced. Manifold/reflector assembly is advantageously used in the system that facilitates electrical circuit for electrophoresis and for gel replacement.

公开(公告)号：EP0763198A1

公开(公告)日：1997-03-19

申请号：EP96909890.0

申请日：1996-03-29

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： RAMPAL, Sushma ,  RAMPAL, Jang, B. ,  OSBORNE, James, C.

IPC: G01N27

CPC classification number: G01N27/44743

Abstract: A sample having both positively and negatively charged components may be separated during the same run by injecting a plug of sample at both ends of the capillary and applying an electric field in the capillary, where there is insufficient electroosmotic flow in the capillary to reverse sample migration direction, such as in the case of a gel-filled column.