公开(公告)号：WO9521270A3

公开(公告)日：1995-10-19

申请号：PCT/US9501468

申请日：1995-02-03

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/686 ,  C12Q1/6862

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

公开(公告)号：WO9403630A3

公开(公告)日：1994-03-31

申请号：PCT/US9307342

申请日：1993-08-04

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  DANIELS DAVID W

IPC: C12N15/09 ,  C12Q1/68 ,  C12Q

CPC classification number: C12Q1/6844 ,  C12Q1/6858 ,  C12Q1/6862 ,  C12Q2533/107 ,  C12Q2533/101 ,  C12Q2525/186

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

公开(公告)号：ES2110009T3

公开(公告)日：1998-02-01

申请号：ES92917536

申请日：1992-07-31

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  WANG EVA Y

IPC: C07K14/195 ,  C07K14/00 ,  C07K14/315 ,  C07K14/41 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N15/09 ,  C12N15/31 ,  C12R1/19 ,  C12R1/46 ,  G01N33/50 ,  C12N5/10

Abstract: Disclosed herein are derivatives of Streptolysin 0 produced by recombinant DNA techniques. In an embodiment, the derivative is soluble upon expression and has a specific hemolytic activity of about 3.6x104 hemolytic units per milligram.

公开(公告)号：AU676197B2

公开(公告)日：1997-03-06

申请号：AU4802793

申请日：1993-08-04

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  DANIELS DAVID W

IPC: C12N15/09 ,  C12Q1/68

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

公开(公告)号：PL307341A1

公开(公告)日：1995-05-15

申请号：PL30734193

申请日：1993-08-04

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  DANIELS DAVID W

IPC: C12Q1/68 ,  C12N15/09 ,  C12Q1/00

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

公开(公告)号：NO950402A

公开(公告)日：1995-04-03

申请号：NO950402

申请日：1995-02-03

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  DANIELS DAVID W

IPC: C12Q20060101 ,  C12Q

公开(公告)号：AU4802793A

公开(公告)日：1994-03-03

申请号：AU4802793

申请日：1993-08-04

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  DANIELS DAVID W

IPC: C12N15/09 ,  C12Q1/68

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

公开(公告)号：CA2094245C

公开(公告)日：2007-01-09

申请号：CA2094245

申请日：1992-07-31

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  WANG EVA Y

IPC: C07K14/195 ,  C12N15/31 ,  C07K14/00 ,  C07K14/315 ,  C07K14/41 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/63 ,  C12R1/19 ,  C12R1/46 ,  G01N33/50

Abstract: Disclosed herein are derivatives of Streptolysin O produced by recombinant D NA techniques. In an embodiment, the derivative is soluble upon expression and has a specific hemolytic activity of about 3.6 .times. 10 4 hemolytic units per milligram.

公开(公告)号：AT160017T

公开(公告)日：1997-11-15

申请号：AT91912973

申请日：1991-06-05

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W

IPC: G01N33/53 ,  A61K31/70 ,  A61K39/395 ,  A61K49/00 ,  A61P31/12 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N5/00 ,  C12N5/10 ,  C12N15/02 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/532 ,  G01N33/566 ,  G01N33/577 ,  G01N33/58

Abstract: Nucleic acid probes and protein probes are disclosed. The nucleic acid probe comprises a probe polynucleotide, a charged hapten label, and a binding moiety. The protein probe comprises a probe protein, a charged hapten label and a binding moiety. The charged hapten label joint to the binding moiety can comprise a negatively charged sulfophenylhydrazine tag compound. Polyclonal antibodies and monoclonal antibodies with specific affinity for the charged hapten labels are disclosed as are hybridomas capable of making the monoclonal antibodies. Methods and kits are disclosed for making the nucleic acid probes, making the protein probes, detecting a capture polynucleotide of the nucleic acid probe and detecting a capture molecule of the protein probe.

公开(公告)号：AU661028B2

公开(公告)日：1995-07-13

申请号：AU2437292

申请日：1992-07-31

Applicant: BECKMAN INSTRUMENTS INC

Inventor： ADAMS CRAIG W ,  WANG EVA Y

IPC: C07K14/195 ,  C07K14/00 ,  C07K14/315 ,  C07K14/41 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N15/09 ,  C12N15/31 ,  C12R1/19 ,  C12R1/46 ,  G01N33/50 ,  C07K13/00 ,  C12N5/10

Abstract: Disclosed herein are derivatives of Streptolysin 0 produced by recombinant DNA techniques. In an embodiment, the derivative is soluble upon expression and has a specific hemolytic activity of about 3.6x104 hemolytic units per milligram.