公开(公告)号：EP3161157A4

公开(公告)日：2017-12-20

申请号：EP15790435

申请日：2015-06-24

Applicant: BIO-RAD LABORATORIES INC

Inventor： AGRESTI JEREMY ,  COOPER SAMANTHA ,  KARLIN-NEUMANN GEORGE ,  HEREDIA NICK ,  LEBOFSKY RONALD

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2521/531 ,  C12Q2525/161 ,  C12Q2525/204 ,  C12Q2535/122 ,  C12Q2565/629 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Methods, compositions, and kits are provided for nucleic acid analysis, including single cell analysis.

公开(公告)号：EP2971160A4

公开(公告)日：2016-12-07

申请号：EP14770183

申请日：2014-03-13

Applicant: BIO-RAD LABORATORIES INC

Inventor： REGAN JOHN ,  COOPER SAMANTHA ,  SO AUSTIN ,  KOEHLER RYAN ,  MONTESCLAROS LUZ

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q2545/114 ,  C12Q2561/101 ,  C12Q2563/159

Abstract: Provided herein are methods, compositions, and kits for detecting alleles using a single probe or a single primer set. Also, provided herein are methods, compositions, and kits for detecting allelic variants using a single probe or a single primer set. Also, provided herein are methods, compositions, and kits for determining a polymerization error rate.

公开(公告)号：EP3033445A4

公开(公告)日：2017-03-22

申请号：EP14836787

申请日：2014-08-12

Applicant: BIO-RAD LABORATORIES INC

Inventor： REGAN JOHN F ,  MAAR DIANNA ,  SHELTON DAWN N ,  LEGLER TINA C ,  COOPER SAMANTHA ,  YANG WEI ,  HEFNER ELI A ,  KLITGORD NIELS

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract translation: 用扩增报道分子进行扩增测定的系统，包括能够在报道分子的解链温度以下彼此碱基配对的第一寡聚物和第二寡聚物。 报道分子可能具有可检测的光致发光，其通过第一和第二寡聚体彼此碱基配对而受到影响，例如减少。 诸如核酸靶序列之类的靶可以在熔解温度以上的至少一个体积（诸如多个分区）中被扩增，并且报道子的光致发光可以从低于解链温度的至少一个体积中被检测到 。 可以基于检测到的光致发光来确定目标的属性，诸如目标的浓度。