公开(公告)号：SG10201710049RA

公开(公告)日：2018-01-30

申请号：SG10201710049R

申请日：2014-03-07

Applicant: BIO-RAD LABORATORIES INC

Inventor： LOWE ADAM ,  MCDERMOTT GEOFF ,  HINDSON CHRIS ,  CHIA ERIN ,  HIDDESSEN AMY ,  HINDSON BEN ,  HAN CHUNXIAO ,  WANG YAQI

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.