公开(公告)号：EP3024843A4

公开(公告)日：2017-03-29

申请号：EP14830330

申请日：2014-07-24

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM

IPC: C07K1/16 ,  A61K38/02 ,  C07K1/18

CPC classification number: C07K1/1136 ,  C07K1/22

Abstract: Compositions, methods, articles of manufacture, and kits are provided for storage and delivery of proteins.

Abstract translation: 组合物，方法，制造的物品和试剂盒提供了用于存储和蛋白质的交付。

公开(公告)号：EP2986968A4

公开(公告)日：2017-03-22

申请号：EP15758608

申请日：2015-03-09

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM

IPC: G01N21/00 ,  G01N21/75 ,  G01N33/559

CPC classification number: B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/065 ,  C12Q1/6806 ,  G01N27/44717 ,  G01N27/44739 ,  G01N33/54366

Abstract: Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.

公开(公告)号：EP2986646A4

公开(公告)日：2017-04-12

申请号：EP14834424

申请日：2014-08-08

Applicant: BIO-RAD LABORATORIES INC

Inventor： MCKEE CLAYTON T ,  STRONG WILLIAM ,  OLECH LEE ,  BERKELMAN THOMAS ,  BELISLE CHRISTOPHER

IPC: G01N33/58 ,  G01N27/447 ,  G01N33/68

CPC classification number: G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

Abstract: A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.