公开(公告)号：CA2826748C

公开(公告)日：2020-08-04

申请号：CA2826748

申请日：2012-02-09

Applicant: BIO-RAD LABORATORIES INC

Inventor： REGAN JOHN FREDERICK ,  SAXONOV SERGE ,  LUCERO MIKE ,  HINDSON BEN ,  BELGRADER PHIL ,  DUBE SIMANT ,  SO AUSTIN ,  MELLEN JEFFREY CLARK ,  HEREDIA NICHOLAS JACK ,  NESS KEVIN ,  COLSTON BILL

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/683 ,  C12Q1/686 ,  C40B30/00

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

公开(公告)号：EP3049559A4

公开(公告)日：2017-05-03

申请号：EP14848062

申请日：2014-09-26

Applicant: BIO-RAD LABORATORIES INC

Inventor： REGAN JOHN FREDERICK ,  TZONEV SVILEN

IPC: C12Q1/68 ,  C12P19/34 ,  C40B30/04 ,  C40B40/06

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6869 ,  G06F19/70 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/107

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable directional chromosome mapping e.g., using chromosome phasing/haplotyping. The improved methods, compositions, and kits can also enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract translation: 本文提供了用于分析核酸的改进的方法，组合物和试剂盒。 改进的方法，组合物和试剂盒可以使定向染色体作图，例如使用染色体定相/单元型分型。 改进的方法，组合物和试剂盒也可以实现样品中核酸的拷贝数估计。 本文还提供了用于确定样品中两个或更多个拷贝的靶核酸的连接（例如，两个或更多个拷贝是在相同染色体上还是不同染色体上）或用于定相等位基因的方法，组合物和试剂盒。